US4904391A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method and apparatus for removal of cells from bone marrow
Assignee: University of Florida
•
Filing: 1985-10-09
•
Granted: 1990-02-27
•
Expires: 2005-10-09
Abstract
Abstract An improved system for removing cells from bone marrow wherein the cells are bound by monoclonal antibodies conjugated to magnetic particles, the system comprising a chamber provided with inlets and outlets for flowing through the chamber a liquid sample containing the bone marrow and magnetic conjugated antibodies bound to the cells and a magnetic field source associated with the chamber wherein the improvement comprises a non-uniform magnetic field in an ascending gradient from the inlet to the outlet.
Inventors
- 👤 Richard B. Freeman
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 23:04
CPC Classifications
B; B03; B03C; B03C1/00; B03C1/02; B03C1/035; B03C1/005; B03C1/01; C; C12; C12M; C12M47/00; C12M47/04; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54313; G01N33/54326; G01N33/569; G01N33/56966; G01N33/56972; G01N35/00; G01N35/0098
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
109 words
In a method for removing cells from bone marrow wherein said cells, bound to monoclonal antibodies, conjugated to magnetic particles, said method comprising flowing a liquid sample containing said bone marrow and magnetic conjugated antibodies bound to said cells through a zone provided with an inlet and an outlet associated with a magnetic field such that said liquid sample flowing there through and passes through said magnetic field whereby said bound cells and unconjugated particles are removed from said liquid sample by magnetic attraction, the improvement wherein: said magnetic field through which said liquid sample flows is non-uniform and is an ascending gradient from said inlet to said outlet.
Claim 2
Dependent Claim
109 words
In a method for removing cells from bone marrow wherein said cells, bound to monoclonal antibodies, conjugated to magnetic particles, said method comprising flowing a liquid sample containing said bone marrow and magnetic conjugated antibodies bound to said cells through a zone provided with an inlet and an outlet associated with a magnetic field such that said liquid sample flowing there through and passes through said magnetic field whereby said bound cells and unconjugated particles are removed from said liquid sample by magnetic attraction, the improvement wherein: said magnetic field through which said liquid sample flows is non-uniform and is an ascending gradient from said inlet to said outlet.
Claim 3
Dependent Claim
10 words
The method of claim 1 wherein said particles are microspheres.
Claim 4
Dependent Claim
10 words
The method of claim 2 wherein said microspheres contain magnetite.
Claim 5
Dependent Claim
10 words
The method of claim 2 wherein said microspheres comprise polystyrene.
Claim 6
Dependent Claim
10 words
The method of claim 1 wherein said cells are malignant.
Claim 7
Dependent Claim
158 words
In a system for removing cells from bone marrow wherein said cells are bound by monoclonal antibodies, conjugated to magnetic particles, said system comprising (1) means defining a chamber provided with inlet and outlet means adapted to enable to flow through said chamber of a liquid sample containing said bone marrow and magnetic conjugated antibodies bound to said cells and (2) at least one magnetic field source associated with said chamber such that said liquid sample flowing therethrough passes through said magnetic field whereby said bound cells are removed from said liquid sample by magnetic attraction, the improvement wherein: said at least one magnetic field source comprises an assembly of plural magnets arranged such that said magnetic field is non-uniform and is an ascending gradient from said inlet means to said outlet means. said improvement further comprising means for eliminating movement in said chamber of said conjugated and any unconjugated magnetic particles after removal from said liquid sample.
Claim 8
Dependent Claim
158 words
In a system for removing cells from bone marrow wherein said cells are bound by monoclonal antibodies, conjugated to magnetic particles, said system comprising (1) means defining a chamber provided with inlet and outlet means adapted to enable to flow through said chamber of a liquid sample containing said bone marrow and magnetic conjugated antibodies bound to said cells and (2) at least one magnetic field source associated with said chamber such that said liquid sample flowing therethrough passes through said magnetic field whereby said bound cells are removed from said liquid sample by magnetic attraction, the improvement wherein: said at least one magnetic field source comprises an assembly of plural magnets arranged such that said magnetic field is non-uniform and is an ascending gradient from said inlet means to said outlet means. said improvement further comprising means for eliminating movement in said chamber of said conjugated and any unconjugated magnetic particles after removal from said liquid sample.
Claim 9
Dependent Claim
33 words
The system of claim 6 wherein said movement elimination means is positioned so as to retard the flow of said conjugated and any unconjugated magnetic particles after capture thereof by said magnetic field.
Claim 10
Dependent Claim
30 words
The system of claim 7 wherein said movement elimination means comprises a mesh element adapted to prevent the escape of said conjugated and any unconjugated particles from said magnetic field.
Claim 11
Dependent Claim
34 words
The system of claim 8 wherein said mesh element is positioned adjacent the wall or walls of said chamber to which said conjugated or any unconjugated magnetic particles are attracted by said magnetic field.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-02-26 | 1993-08-28 | 1994-02-27 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-02-26 | 1997-08-28 | 1998-02-27 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-02-26 | 2001-08-28 | 2002-02-27 | $7700.00 | Window Closed / Lapsed |