US4895805A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Cell manipulating apparatus
Assignee: Hitachi Ltd
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Filing: 1988-08-24
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Granted: 1990-01-23
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Expires: 2008-08-24
Abstract
Abstract Disclosed is a cell manipulating apparatus for conveying cells to a plurality of chambers provided in a chamber plate. The apparatus comprises a taking-up device adapted to take up cells to the respective taking up portions of a carrier plate placed in positions corresponding to those of said chambers in the chamber plate and a moving device adapted to move, relatively with respect to the chamber plate, the taking-up device to a position above the chamber plate. The taking-up portions may be effectively composed of pressure-controlled cavities or SiO 2 films provided in the carrier plate.
Inventors
- 👤 Kazuo Sato
- 👤 Shinji Tanaka
- 👤 Yoshio Kawamura
- 👤 Hiroyuki Kohida
- 👤 Yoshihisa Hosoe
- 👤 Masatoshi Sakurano
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 12:27
CPC Classifications
C; C12; C12M; C12M33/00; C12M33/04; C12M23/00; C12M23/02; C12M23/12; C12M23/34; C12M23/58; C12M35/00; C12M35/08
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
115 words
A cell manipulating apparatus for conveying cells to a plurality of chambers provided in a chamber plate, comprising: a taking-up device including a carrier plate having taking-up portions placed in positions corresponding to those of said chambers in said chamber plate, each of said taking-up portions being adapted to provide means for taking up a single cell and a moving device adapted to move, relative with respect to the chamber plate, said taking-up device to a position above said chamber plate to set said taking-up portions in close proximity with respect to said chambers to allow transfer of a plurality of said single cells from said taking-up device to said chamber plate at one time.
Claim 2
Dependent Claim
115 words
A cell manipulating apparatus for conveying cells to a plurality of chambers provided in a chamber plate, comprising: a taking-up device including a carrier plate having taking-up portions placed in positions corresponding to those of said chambers in said chamber plate, each of said taking-up portions being adapted to provide means for taking up a single cell and a moving device adapted to move, relative with respect to the chamber plate, said taking-up device to a position above said chamber plate to set said taking-up portions in close proximity with respect to said chambers to allow transfer of a plurality of said single cells from said taking-up device to said chamber plate at one time.
Claim 3
Dependent Claim
18 words
A cell manipulating apparatus as claimed in claim 1, wherein a plurality of said taking-up devices are provided.
Claim 4
Dependent Claim
89 words
A cell manipulating apparatus for conveying cells to a plurality of chambers provided in the chamber plate, comprising: a taking-up device adapted to take up cells to taking-up portions in a carrier plate placed in positions corresponding to those of said chambers in said chamber plate and a moving device adapted to move, relative with respect to the chamber plate, said taking-up device to a position above said chamber plate; both or either of said chamber plate and said carrier plate are or is formed from single crystal silicon.
Claim 5
Dependent Claim
89 words
A cell manipulating apparatus for conveying cells to a plurality of chambers provided in the chamber plate, comprising: a taking-up device adapted to take up cells to taking-up portions in a carrier plate placed in positions corresponding to those of said chambers in said chamber plate and a moving device adapted to move, relative with respect to the chamber plate, said taking-up device to a position above said chamber plate; both or either of said chamber plate and said carrier plate are or is formed from single crystal silicon.
Claim 6
Dependent Claim
173 words
A cell manipulating apparatus for conveying cells to a plurality of chambers provided in a chamber plate, comprising: a taking-up device having a carrier plate including cavities and penetrating holes provided in positions corresponding to those of the chambers of said chamber plate and a pressure control means for controlling liquid pressure within said penetrating holes, said taking-up device having means adapted to take up and to release a single cell to and from each of said cavities, respectively; a container in which said carrier plate is to be placed and into which buffer solution and the cells are introduced; a cell feeding means for feeding the cells into said container; and a moving device adapted to move, relatively with respect to said chamber plate, said taking-up device which has taken up the single cells to a position above said chamber plate to allow transfer of a plurality of said single cells from said taking-up device to said chamber plate at one time by a change in liquid pressure within said penetrating holes.
Claim 7
Dependent Claim
173 words
A cell manipulating apparatus for conveying cells to a plurality of chambers provided in a chamber plate, comprising: a taking-up device having a carrier plate including cavities and penetrating holes provided in positions corresponding to those of the chambers of said chamber plate and a pressure control means for controlling liquid pressure within said penetrating holes, said taking-up device having means adapted to take up and to release a single cell to and from each of said cavities, respectively; a container in which said carrier plate is to be placed and into which buffer solution and the cells are introduced; a cell feeding means for feeding the cells into said container; and a moving device adapted to move, relatively with respect to said chamber plate, said taking-up device which has taken up the single cells to a position above said chamber plate to allow transfer of a plurality of said single cells from said taking-up device to said chamber plate at one time by a change in liquid pressure within said penetrating holes.
Claim 8
Dependent Claim
163 words
A cell manipulating apparatus for conveying cells to a plurality of chambers provided in a chamber plate, comprising: a taking-up device having a carrier plate including cavities and penetrating holes provided in positions corresponding to those of the chambers of said chamber plate and a pressure control means for controlling the pressure within said penetrating holes, said taking-up device being adapted to take up the cells to said cavities; a container in which said carrier plate is to be placed and into which buffer solution and the cells are introduced; a cell feeding means for feeding the cells into said chamber and a moving device adapted to move, relatively with respect to said chamber plate, said taking-up device which has taken up the cells to a position above said chamber plate; said pressure control means being composed of a plate holder conveying the upper surface of said carrier plate and a means for lowering the pressure at a region in said plate holder.
Claim 9
Dependent Claim
163 words
A cell manipulating apparatus for conveying cells to a plurality of chambers provided in a chamber plate, comprising: a taking-up device having a carrier plate including cavities and penetrating holes provided in positions corresponding to those of the chambers of said chamber plate and a pressure control means for controlling the pressure within said penetrating holes, said taking-up device being adapted to take up the cells to said cavities; a container in which said carrier plate is to be placed and into which buffer solution and the cells are introduced; a cell feeding means for feeding the cells into said chamber and a moving device adapted to move, relatively with respect to said chamber plate, said taking-up device which has taken up the cells to a position above said chamber plate; said pressure control means being composed of a plate holder conveying the upper surface of said carrier plate and a means for lowering the pressure at a region in said plate holder.
Claim 10
Dependent Claim
18 words
A cell manipulating apparatus as claimed in claim 4, wherein said cell feeding means comprises an injection apparatus.
Claim 11
Dependent Claim
141 words
A cell manipulating apparatus for conveying cells to a plurality of chambers provided in a chamber plate, comprising: a taking-up device having a carrier plate including cavities and penetrating holes provided in positions corresponding to those of the chambers of said chamber plate and a pressure control means for controlling the pressure within said penetrating holes, said taking-up device being adapted to take up the cells to said cavities; a container in which said carrier plate is to be placed and into which buffer solution and the cells are introduced; a cell feeding means for feeding the cells into said chamber and a moving device adapted to move, relatively with respect to said chamber plate, said taking-up device which has taken up the cells to a position above said chamber plate; said carrier plate being formed from single crystal silicon wafer.
Claim 12
Dependent Claim
141 words
A cell manipulating apparatus for conveying cells to a plurality of chambers provided in a chamber plate, comprising: a taking-up device having a carrier plate including cavities and penetrating holes provided in positions corresponding to those of the chambers of said chamber plate and a pressure control means for controlling the pressure within said penetrating holes, said taking-up device being adapted to take up the cells to said cavities; a container in which said carrier plate is to be placed and into which buffer solution and the cells are introduced; a cell feeding means for feeding the cells into said chamber and a moving device adapted to move, relatively with respect to said chamber plate, said taking-up device which has taken up the cells to a position above said chamber plate; said carrier plate being formed from single crystal silicon wafer.
Claim 13
Dependent Claim
141 words
A cell manipulating apparatus for conveying cells to a plurality of chambers provided in a chamber plate, comprising: a taking-up device having a carrier plate including cavities and penetrating holes provided in positions corresponding to those of the chambers of said chamber plate and a pressure control means for controlling the pressure within said penetrating holes, said taking-up device being adapted to take up the cells to said cavities; a container in which said carrier plate is to be placed and into which buffer solution and the cells are introduced; a cell feeding means for feeding the cells into said chamber and a moving device adapted to move, relatively with respect to said chamber plate, said taking-up device which has taken up the cells to a position above said chamber plate; said chamber plate being formed from single crystal silicon wafer.
Claim 14
Dependent Claim
141 words
A cell manipulating apparatus for conveying cells to a plurality of chambers provided in a chamber plate, comprising: a taking-up device having a carrier plate including cavities and penetrating holes provided in positions corresponding to those of the chambers of said chamber plate and a pressure control means for controlling the pressure within said penetrating holes, said taking-up device being adapted to take up the cells to said cavities; a container in which said carrier plate is to be placed and into which buffer solution and the cells are introduced; a cell feeding means for feeding the cells into said chamber and a moving device adapted to move, relatively with respect to said chamber plate, said taking-up device which has taken up the cells to a position above said chamber plate; said chamber plate being formed from single crystal silicon wafer.
Claim 15
Dependent Claim
97 words
A cell manipulating apparatus for conveying cells to a plurality of chambers provided in a chamber plate, comprising: a taking-up device composed of a carrier plate formed from single crystal silicon and taking-up portions of SiO 2 films arranged on said carrier plate such as to be in positions corresponding to those of the chambers of said chamber plate, said taking-up device being adapted to take up said cells on said taking-up portions, and a moving device adapted to move, relatively with respect to said chamber plate, said taking-up device to a position above said chamber plate.
Claim 16
Dependent Claim
97 words
A cell manipulating apparatus for conveying cells to a plurality of chambers provided in a chamber plate, comprising: a taking-up device composed of a carrier plate formed from single crystal silicon and taking-up portions of SiO 2 films arranged on said carrier plate such as to be in positions corresponding to those of the chambers of said chamber plate, said taking-up device being adapted to take up said cells on said taking-up portions, and a moving device adapted to move, relatively with respect to said chamber plate, said taking-up device to a position above said chamber plate.
Claim 17
Dependent Claim
20 words
A cell manipulating apparatus as claimed in claim 9, wherein said chamber plate is formed from single crystal silicon wafer.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-01-22 | 1993-07-24 | 1994-01-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-01-22 | 1997-07-24 | 1998-01-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-01-22 | 2001-07-24 | 2002-01-23 | $7700.00 | Window Closed / Lapsed |