US4888324A
Expired / Lapsed
US
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Method for enhancing angiogenesis with lipid containing molecules
Assignee: Boston University
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Filing: 1987-11-02
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Granted: 1989-12-19
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Expires: 2007-11-02
Abstract
Abstract Compositions which contain lipid containing molecules possessing angiogenic activity are disclosed. The lipid containing molecules have been found in mammalian tissue, particularly the omentum, of various animals. Additionally, known lipids, such as gangliosides, are unexpectedly found to possess angiogenic activity. A method for obtaining the omentum-derived lipids, and for use of these and known lipids, are disclosed as well.
Inventors
- 👤 Nicholas Catsimpoolas
- 👤 Robert McCluer
- 👤 Robert S. Sinn
- 👤 James Evans
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 22:55
CPC Classifications
A; A61; A61K; A61K31/00; A61K31/66; A61K31/683; A61K31/685; A61K31/70; A61K31/715
Claims Analysis
Total Claims: 24
Claim 1
Independent Claim
92 words
A process for obtaining a composition with enhanced angiogenic acitivity comprising containing a sample of mammalian tissue with a first solvent under conditions favoring extraction of angiogenically active lipid containing molecules selected from the group consisting of phospholipids, ceramides, cerebrosides, neutral lipids, triglycerides, diglycerides, monoglycerides lecithin and sphingosides into said first solvent and further contacting said angiogenically active lipid containing first solvent with a second solvent under conditions favoring extraction of said angiogenically active lipid containing molecules but not angiogenic inhibiting substances, said first and second solvents being insoluble in each other.
Claim 2
Dependent Claim
92 words
A process for obtaining a composition with enhanced angiogenic acitivity comprising containing a sample of mammalian tissue with a first solvent under conditions favoring extraction of angiogenically active lipid containing molecules selected from the group consisting of phospholipids, ceramides, cerebrosides, neutral lipids, triglycerides, diglycerides, monoglycerides lecithin and sphingosides into said first solvent and further contacting said angiogenically active lipid containing first solvent with a second solvent under conditions favoring extraction of said angiogenically active lipid containing molecules but not angiogenic inhibiting substances, said first and second solvents being insoluble in each other.
Claim 3
Dependent Claim
14 words
A process as in claim 1, wherein said first solvent is a chloroform-methanol mixture.
Claim 4
Dependent Claim
15 words
A process as in claim 1, wherein said second solvent is a hexane ethanol mixture.
Claim 5
Dependent Claim
22 words
A process as in claim 1, wherein said first solvent is a chloroform-methanol mixture and said second solvent is a hexane-ethanol mixture.
Claim 6
Dependent Claim
20 words
Process as in claim 1, wherein said angiogenically active lipid-containing second solvent is separated into component hexane and ethanol phases.
Claim 7
Dependent Claim
28 words
Process as in claim 5, wherein said ethanol phase is partitioned into an upper and a lower phase by combining with a mixture of chloroform, methanol, and water.
Claim 8
Dependent Claim
35 words
Process as in claim 6, wherein said upper phase is separated into two phases by combining said upper phase, in sequence, with a mixture of methanol and water, and a mixture of chloroform and methanol.
Claim 9
Dependent Claim
23 words
A process as in claim 6, wherein said lower phase is mixed, sequentially, with chloroform, acetone, and methanol, to form three separate portions.
Claim 10
Dependent Claim
24 words
Method as in claim 6, wherein said lower phase is contacted with separate portions of chloroform, acetone, and methanol to form separate fractions thereof.
Claim 11
Dependent Claim
47 words
Process of claim 6, wherein said upper phase is further contacted with a first mixture of methanol and water and a second mixture of chloroform and methanol to form a solvent of chloroform and methanol containing said angiogenically active lipid containing molecules but not non-lipid containing molecules.
Claim 12
Dependent Claim
26 words
Process of claim 10, further comprising contacting said solvent with a solvent mixture of chloroform methanol and water to obtain non-ganglioside angiogenically active lipid containing molecules.
Claim 13
Independent Claim
52 words
A method of enhancing angiogenesis in a patient comprising applying to said patient a therapeutically effective amount of a composition containing an angiogenically active lipid containing molecule selected from the group consisting of a phospholipid, a ceramide, a cerebroside, a neutral lipid, a triglyceride, a diglyceride, amonoglyceride, a lecithin, and a sphingoside.
Claim 14
Independent Claim
52 words
A method of enhancing angiogenesis in a patient comprising applying to said patient a therapeutically effective amount of a composition containing an angiogenically active lipid containing molecule selected from the group consisting of a phospholipid, a ceramide, a cerebroside, a neutral lipid, a triglyceride, a diglyceride, amonoglyceride, a lecithin, and a sphingoside.
Claim 15
Dependent Claim
13 words
Method of claim 4, wherein said composition is applied orally, intravenously, or topically.
Claim 16
Dependent Claim
15 words
The method of claim 12, wherein said angiogenically active lipid containing molecule is a phospholipid.
Claim 17
Dependent Claim
15 words
The method of claim 12, wherein said angiogenically active lipid containing molecule is a ceramide.
Claim 18
Dependent Claim
16 words
The method of claim 12, wherein said angiogenically active lipid containing a molecule is a cerebroside.
Claim 19
Dependent Claim
16 words
The method of claim 12, wherein said angiogenically active lipid containing molecule is a neutral lipid.
Claim 20
Dependent Claim
15 words
The method of claim 12, wherein said angiogenically active lipid containing molecule is a triglyceride.
Claim 21
Dependent Claim
15 words
The method of claim 12, wherein said angiogenically active lipid containing molecule is a diglyceride.
Claim 22
Dependent Claim
15 words
The method of claim 12, wherein said angiogenically active lipid containing molecule is a monoglyceride.
Claim 23
Dependent Claim
15 words
The method of claim 12, wherein said angiogenically active lipid containing molecule is a lecithin.
Claim 24
Dependent Claim
15 words
The method of claim 12, wherein said angiogenically active lipid containing molecule is a sphingoside.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1992-12-18 | 1993-06-19 | 1993-12-19 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-12-18 | 1997-06-19 | 1997-12-19 | $3760.00 | Window Closed / Lapsed |
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