US4883755A
Expired / Lapsed
US
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Method of reendothelializing vascular linings
Assignee: Thomas Jefferson University
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Filing: 1987-10-28
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Granted: 1989-11-28
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Expires: 2007-10-28
Abstract
Abstract A method for treating the vascular passage of a patient, damaged by procedures such as an endarterectomy which denude portions of the vascular passages of their endothelial cell linings, is disclosed. In this method, endothelial cells are isolated from the patient's own microvessels, the flow of blood through the patient's damaged vascular passage is interrupted, the endothelial cells isolated from the patient's microvessels are applied to the surface of the denuded portion of the patient's vascular passage in a density sufficient to provide covereage of at least about 50% of said denuded portion, and interruption of blood flow through the vascular passage is maintained for a period of time sufficient to allow the sodded cells to form an attachment to the vascular lining sufficient to withstand the shear created by resumed blood flow through the vascular passage.
Inventors
- 👤 R. Anthony Carabasi
- 👤 Bruce E. Jarrell
- 👤 Stuart K. Williams
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 05:16
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/06; C12N5/0602; C12N5/069; A; A61; A61F; A61F2/00; A61F2/02; A61F2/04; A61F2/06; A61F2/062; A61K; A61K35/00; A61K35/12; A61K35/14; A61K35/16; A61K35/44; A61L; A61L27/00; A61L27/36; A61L27/50; A61L27/507; A61P; A61P9/00; A61P9/14
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
111 words
A method of reendothelializing the vascular passage of a patient, the lining of which has been substantially denuded of endothelial cells, comprising (a) interrupting the flow of blood through said vascular passage, (b) applying to said denuded vascular lining uncultured endothelial cells retrieved from the microvessels of said patient in a density sufficient to provide coverage of at least about 50% of said denuded portion; and (c) maintaining the interruption of blood flow through said vascular passage for a period of time sufficient to allow 5 said applied cells to form an attachment to said vascular lining sufficient to withstand the shear created by resumed blood flow through said vascular passage.
Claim 2
Independent Claim
111 words
A method of reendothelializing the vascular passage of a patient, the lining of which has been substantially denuded of endothelial cells, comprising (a) interrupting the flow of blood through said vascular passage, (b) applying to said denuded vascular lining uncultured endothelial cells retrieved from the microvessels of said patient in a density sufficient to provide coverage of at least about 50% of said denuded portion; and (c) maintaining the interruption of blood flow through said vascular passage for a period of time sufficient to allow 5 said applied cells to form an attachment to said vascular lining sufficient to withstand the shear created by resumed blood flow through said vascular passage.
Claim 3
Dependent Claim
27 words
The method of claim 1 where said endothelial cells are applied in a density of at least about 1×10 5 cells per cm 2 of seeded surface.
Claim 4
Dependent Claim
27 words
The method of claim 1 where said endothelial cells are applied in a density sufficient to form a confluent layer of said cells on said vascular lining.
Claim 5
Dependent Claim
22 words
The method of claim 1 where said endothelial cells are applied to said vascular passage in a suspension of blood or plasma.
Claim 6
Dependent Claim
22 words
The method of claim 2 where said endothelial cells are applied to said vascular passage in a suspension of blood or plasma.
Claim 7
Dependent Claim
22 words
The method of claim 3 where said endothelial cells are applied to said vascular passage in a suspension of blood or plasma.
Claim 8
Dependent Claim
23 words
The method of claim 1 where said endothelial cells are applied to said vascular passage in a suspension of a non-gel-forming buffer solution.
Claim 9
Dependent Claim
23 words
The method of claim 2 where said endothelial cells are applied to said vascular passage in a suspension of a non-gel-forming buffer solution.
Claim 10
Dependent Claim
23 words
The method of claim 3 where said endothelial cells are applied to said vascular passage in a suspension of a non-gel-forming buffer solution.
Claim 11
Dependent Claim
18 words
The method of claim 1 where said endothelial cells are derived from the fat tissues of the patient.
Claim 12
Dependent Claim
18 words
The method of claim 4 where said endothelial cells are derived from the fat tissues of the patient.
Claim 13
Dependent Claim
18 words
The method of claim 7 where said endothelial cells are derived from the fat tissues of the patient.
Claim 14
Dependent Claim
26 words
The method of claim 1 where said endothelial cells are retrieved from said patient and applied to said patient's vascular lining during the sam operative procedure.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-11-27 | 1993-05-29 | 1993-11-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-11-27 | 1997-05-29 | 1997-11-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-11-27 | 2001-05-29 | 2001-11-28 | $7700.00 | Window Closed / Lapsed |