US4879237A
Expired / Lapsed
US
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Use of peptides in control of cell attachment and detachment
Assignee: Sanford Burnham Prebys Medical Discovery Institute
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Filing: 1985-05-24
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Granted: 1989-11-07
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Expires: 2005-05-24
Abstract
Abstract A method of using synthetic cell attachment-promoting peptides from fibronectin to detach cultured cells from the substratum is described.
Inventors
- 👤 Eric I. Rudslahti
- 👤 Edward G. Hayman
- 👤 Michael D. Pierschbacher
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 20:59
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/0068; C12N2539/00; C12N2539/10
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
52 words
A method of controlling the Arg-Gly-Asp mediated attachment of animal cells to substrates comprising the steps of: (a) contacting a substrate normally capable of accepting cells with cells normally capable of attaching to such substrate; and (b) contacting the substrate with a solution containing a polypeptide consisting essentially of the sequence GLY-ARG-GLY-ASP-ALA-PRO.
Claim 2
Independent Claim
52 words
A method of controlling the Arg-Gly-Asp mediated attachment of animal cells to substrates comprising the steps of: (a) contacting a substrate normally capable of accepting cells with cells normally capable of attaching to such substrate; and (b) contacting the substrate with a solution containing a polypeptide consisting essentially of the sequence GLY-ARG-GLY-ASP-ALA-PRO.
Claim 3
Independent Claim
49 words
A method of preventing the Arg-Gly-Aap mediated attachment of animal cells to a substrate comprising contacting the cells with a polypeptide consisting essentially of the sequence GLY-ARG-GLY-ASP-ALA-PRO either before contacting the substrate with cells capable of attaching to the substrate or simultaneously with contacting such cells to such substrate.
Claim 4
Independent Claim
49 words
A method of preventing the Arg-Gly-Aap mediated attachment of animal cells to a substrate comprising contacting the cells with a polypeptide consisting essentially of the sequence GLY-ARG-GLY-ASP-ALA-PRO either before contacting the substrate with cells capable of attaching to the substrate or simultaneously with contacting such cells to such substrate.
Claim 5
Independent Claim
42 words
A method of producing animal cells comprising the steps of: (a) growing the cells on a substrate; (b) contacting the cells with a solution containing a polypeptide consisting essentially of the sequence GLY-ARG-GLY-ASP- ALA-PRO; and (c) harvesting cells detached from the substrate.
Claim 6
Independent Claim
42 words
A method of producing animal cells comprising the steps of: (a) growing the cells on a substrate; (b) contacting the cells with a solution containing a polypeptide consisting essentially of the sequence GLY-ARG-GLY-ASP- ALA-PRO; and (c) harvesting cells detached from the substrate.
Claim 7
Independent Claim
51 words
A method for detaching animal cells from a substrate to which they are bound in an Arg-Gly-Asp mediated manner, comprising contacting said bound cells with a solution containing a non-naturally occurring peptide consisting essentially of the amino acid sequence Arg-Gly-Asp-Y, is any amino acid such that the peptide has cell-detachment activity.
Claim 8
Independent Claim
51 words
A method for detaching animal cells from a substrate to which they are bound in an Arg-Gly-Asp mediated manner, comprising contacting said bound cells with a solution containing a non-naturally occurring peptide consisting essentially of the amino acid sequence Arg-Gly-Asp-Y, is any amino acid such that the peptide has cell-detachment activity.
Claim 9
Dependent Claim
20 words
The method of claim 4 wherein Y further comprises a Ser, Thr, Cys, or Ala residue adjacent said Asp residue.
Claim 10
Independent Claim
66 words
A method for harvesting animal cells grown on a substrate to which said cells attach in an Arg-Gly-Asp mediated manner comprising the steps of: contacting said bound cells with a solution containing a non-naturally occurring peptide consisting essentially of the amino acid sequence Arg-Gly-Asp-Y, wherein Y is any amino acid such that the peptide has cell detachment promoting activity; and harvesting cells detached from said substrate.
Claim 11
Independent Claim
66 words
A method for harvesting animal cells grown on a substrate to which said cells attach in an Arg-Gly-Asp mediated manner comprising the steps of: contacting said bound cells with a solution containing a non-naturally occurring peptide consisting essentially of the amino acid sequence Arg-Gly-Asp-Y, wherein Y is any amino acid such that the peptide has cell detachment promoting activity; and harvesting cells detached from said substrate.
Claim 12
Dependent Claim
20 words
The method of claim 6 wherein Y further comprises a Ser, Thr, Cyc or Ala residue adjacent said Asp residue.
Claim 13
Independent Claim
60 words
A method of detaching animal cells from a substrate to which they are bound in an Arg-Gly-Asp mediated manner, comprising contacting said bound cells with a peptide consisting essentially of the amino acid sequence X-Arg-Gly-Asp-Y wherein X is zero to thirty amino acids and Y is one to thirty amino acids, such that the peptide has cell detachment promoting activity.
Claim 14
Independent Claim
60 words
A method of detaching animal cells from a substrate to which they are bound in an Arg-Gly-Asp mediated manner, comprising contacting said bound cells with a peptide consisting essentially of the amino acid sequence X-Arg-Gly-Asp-Y wherein X is zero to thirty amino acids and Y is one to thirty amino acids, such that the peptide has cell detachment promoting activity.
Claim 15
Independent Claim
74 words
A method for harvesting animal cells grown on a substrate to which said cells attach in an Arg-Gly-Asp mediated manner, comprising the steps of: contracting said bound cells with a solution containing a peptide consisting essentially of the amino acid sequence X-Arg-Gly-Asp-Y wherein X is zero to thirty amino acids and Y is one to thirty amino acids, such that the peptide has cell detachment promoting activity; and harvesting cells detached from said substrate.
Claim 16
Independent Claim
74 words
A method for harvesting animal cells grown on a substrate to which said cells attach in an Arg-Gly-Asp mediated manner, comprising the steps of: contracting said bound cells with a solution containing a peptide consisting essentially of the amino acid sequence X-Arg-Gly-Asp-Y wherein X is zero to thirty amino acids and Y is one to thirty amino acids, such that the peptide has cell detachment promoting activity; and harvesting cells detached from said substrate.
References Cited (Backward Citations)
US4578079A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-11-06 | 1993-05-08 | 1993-11-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-11-06 | 1997-05-08 | 1997-11-07 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-11-06 | 2001-05-08 | 2001-11-07 | $7700.00 | Window Closed / Lapsed |