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US4876196A Expired / Lapsed US ⏱️ 0.0 years remaining

Method of continuously producing ethanol from sugar-containing substrates

Assignee: Vogelbusch GmbH
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Filing: 1988-01-27
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Granted: 1989-10-24
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Expires: 2008-01-27
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Abstract

Abstract In a method of continuously producing ethanol from sugar-containing substrates by fermentation of sugars by means of a flocculating strain of Zymomonas mobilis under anaerobic conditions and at a pH of from 4.5 to 7 a substrate is led commonly with Zymomonas mobilis cells through at least three fermentation stages without preceding sterilization, a concentration of at least 4% by volume of ethanol is maintained in each fermentation stage, a residence time of the fermentation medium in the entire system of maximally 31/3 h corresponding to a dilution rate of the fermentation medium in the entire system of at least 0.3 h -1 is adjusted, the Zymomonas mobilis cells are separated by sedimentation after the final fermentation stage, the Zymomonas mobilis cells are recycled into the first fermentation stage, and the ethanol-containing substrate separated from the Zymomonas mobilis cells is drawn off.

Inventors

  • 👤 Wolfgang Salzbrunn
  • 👤 Eva Steiner
  • 👤 Wilfried Wohrer
  • 👤 Otto Meixner

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-10-30 22:35
CPC Classifications
C; C12; C12P; C12P7/00; C12P7/02; C12P7/04; C12P7/06; C12P7/065; Y; Y02; Y02E; Y02E50/00; Y02E50/10; Y02P; Y02P20/00; Y02P20/50; Y02P20/582; Y10; Y10S; Y10S435/00; Y10S435/813; Y10S435/8215; Y10S435/822

Claims Analysis

Total Claims: 8
Claim 1
Independent Claim 157 words
A method of continuously producing ethanol from sugar-containing substrates comprising fermentation of the sugar in said substrates by a flocculating strain of Zymomonas mobilis cells under anaerobic conditions, the method consisting essentially of: flowing a fermentation medium comprising said substrates and said Zymomonas mobilis cells through at least three fermentation stages of a fermentation system without preceding sterilization; maintaining a concentration of at least 4% by volume of ethanol and a pH of about 5 in each of said fermentation stages; maintaining a residence time of said fermentation medium in said fermentation stages of up to about 3 h., corresponding to a dilution rate of said fermentation medium in said fermentation stages of at least about 1/3h -1 ; separating the Zymomonas mobilis cells after the final fermentation stage by sedimentation to form ethanol-containing substrates, and recycling said Zymomonas mobilis cells into the first fermentation stage; and removing said ethanol-containing substrates separated from said Zymomonas mobilis cells.
Claim 2
Independent Claim 157 words
A method of continuously producing ethanol from sugar-containing substrates comprising fermentation of the sugar in said substrates by a flocculating strain of Zymomonas mobilis cells under anaerobic conditions, the method consisting essentially of: flowing a fermentation medium comprising said substrates and said Zymomonas mobilis cells through at least three fermentation stages of a fermentation system without preceding sterilization; maintaining a concentration of at least 4% by volume of ethanol and a pH of about 5 in each of said fermentation stages; maintaining a residence time of said fermentation medium in said fermentation stages of up to about 3 h., corresponding to a dilution rate of said fermentation medium in said fermentation stages of at least about 1/3h -1 ; separating the Zymomonas mobilis cells after the final fermentation stage by sedimentation to form ethanol-containing substrates, and recycling said Zymomonas mobilis cells into the first fermentation stage; and removing said ethanol-containing substrates separated from said Zymomonas mobilis cells.
Claim 3
Dependent Claim 49 words
A method as set forth in claim 1, wherein said residence time of said fermentation medium in said fermentation stages is adjusted to from 0.8 to 2.5 h corresponding to a dilution rate of said fermentation medium in said fermentation stages of from 0.4 to 1.25 h -1 .
Claim 4
Dependent Claim 40 words
A method as set forth in claim 1, wherein CO 2 is formed in each one of said at least three fermentation stages and wherein at least a part of the CO 2 formed is circulated in the respective stage.
Claim 5
Dependent Claim 44 words
A method as set forth in claim 1, wherein ethanol is recovered from said drawn-off ethanol-containing substrate and distillery slops remain, and wherein at least a part of said distillery slops is recycled into the first one of said at least three fermentation stages.
Claim 6
Dependent Claim 34 words
A method as set forth in claim 1 wherein additional substrate is added to each stage as required to adapt the ratio of microorganism population to the alcohol and sugar concentration in each stage.
Claim 7
Dependent Claim 24 words
A method as set forth in claim 1 wherein a pH adjusting dent is added to each stage as required to control the pH.
Claim 8
Dependent Claim 32 words
A method as set forth in claim 6 wherein said substrate contains ammonium sulfate, the ammonium ions being taken up by the Zymomonas mobilis organisms, and said pH adjusting agent is lye.

References Cited (Backward Citations)

US4403034A Cited
US4443544A Cited
US4310629A Cited
EP0047641A2 Cited
DE3148329A1 Cited
US4413058A Cited

USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 1992-10-23 1993-04-24 1993-10-24 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 1996-10-23 1997-04-24 1997-10-24 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 2000-10-23 2001-04-24 2001-10-24 $7700.00 Window Closed / Lapsed

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