US4874691A
Expired / Lapsed
US
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Membrane-supported immunoassays
Assignee: Novelion Therapeutics Inc
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Filing: 1987-10-16
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Granted: 1989-10-17
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Expires: 2007-10-16
Abstract
Abstract Improved, economical devices for immunoassays employ a reusable syringe or vacuum manifold to withdraw samples through a membrane containing an affinity partner for analyte. The devices can also be adapted to direct blood sampling and to automated assays.
Inventors
- 👤 Howard Chandler
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 15:44
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/5436; G01N33/54366; G01N33/74; G01N33/76; G01N35/00; G01N35/10; G01N2035/1027; G01N2035/1048; G01N2035/1055; Y; Y10; Y10S; Y10S436/00; Y10S436/807
Claims Analysis
Total Claims: 38
Claim 1
Independent Claim
81 words
A method to conduct an assay for an analyte in a sample, wherein the analyte binds to an affinity partner, which method comprises: drawing a volume of said sample by means of a pressure gradient through a membrane in which the affinity partner for the analyte is contained into a receiving chamber on which said membrane is mounted, wherein said pressure gradient is obtained by creating a partial vacuum in said receiving chamber, and detecting the analyte bound in the membrane.
Claim 2
Independent Claim
81 words
A method to conduct an assay for an analyte in a sample, wherein the analyte binds to an affinity partner, which method comprises: drawing a volume of said sample by means of a pressure gradient through a membrane in which the affinity partner for the analyte is contained into a receiving chamber on which said membrane is mounted, wherein said pressure gradient is obtained by creating a partial vacuum in said receiving chamber, and detecting the analyte bound in the membrane.
Claim 3
Dependent Claim
15 words
The method of claim 1 wherein the receiving chamber is the body of a syringe.
Claim 4
Dependent Claim
13 words
The method of claim 1 wherein the receiving chamber is a vacuum manifold.
Claim 5
Dependent Claim
17 words
The method of claim 1 wherein the affinity partner for the analyte is a specific affinity partner.
Claim 6
Dependent Claim
18 words
The method of claim 1 wherein the affinity partner for the analyte is an immunoglobulin or derivative thereof.
Claim 7
Dependent Claim
17 words
The method of claim 1 wherein the affinity partner for the analyte is an antigen or hapten.
Claim 8
Dependent Claim
20 words
The method of claim 1 wherein the bound analyte is detected by reaction with a labeled immunoglobulin or derivative thereof.
Claim 9
Dependent Claim
18 words
The method of claim 7 wherein the immunoglobulin or derivative thereof is an antibody specific for the analyte.
Claim 10
Dependent Claim
14 words
The method of claim 8 wherein the labeled antibody is labeled with an enzyme.
Claim 11
Dependent Claim
19 words
The method of claim 2 wherein the sample is blood or internal body fluids drawn directly past the membrane.
Claim 12
Independent Claim
108 words
A method to conduct an assay for analyte in two or more samples simultaneously wherein the analyte binds to an affinity partner, which method comprises: placing a volume of each sample in contact with one of a multiplicity of membranes in each of which the affinity partner for the analyte is contained, wherein each of said multiplicity of said membranes is mounted onto a common receiving chamber, and in a single operation withdrawing each sample by means of a pressure gradient applied as a partial vacuum in said receiving chamber through the membrane into said receiving chamber, and detecting the analyte bound in each membrane from each sample.
Claim 13
Independent Claim
108 words
A method to conduct an assay for analyte in two or more samples simultaneously wherein the analyte binds to an affinity partner, which method comprises: placing a volume of each sample in contact with one of a multiplicity of membranes in each of which the affinity partner for the analyte is contained, wherein each of said multiplicity of said membranes is mounted onto a common receiving chamber, and in a single operation withdrawing each sample by means of a pressure gradient applied as a partial vacuum in said receiving chamber through the membrane into said receiving chamber, and detecting the analyte bound in each membrane from each sample.
Claim 14
Dependent Claim
35 words
The method of claim 11 wherein each sample is placed in an outer chamber affixed to the corresponding membrane and wherein said pressure gradient is obtained by creating a partial vacuum in said receiving chamber.
Claim 15
Independent Claim
69 words
An apparatus for the detection of analyte in a sample wherein the detection comprises reaction of said analyte with an affinity partner, which apparatus comprises: a supported membrane containing said affinity partner, wherein said membrane is supported at the upper portion of a housing wherein said housing further includes means to attach said membrane to an orifice of a receiving chamber in which a partial vacuum can be created.
Claim 16
Independent Claim
69 words
An apparatus for the detection of analyte in a sample wherein the detection comprises reaction of said analyte with an affinity partner, which apparatus comprises: a supported membrane containing said affinity partner, wherein said membrane is supported at the upper portion of a housing wherein said housing further includes means to attach said membrane to an orifice of a receiving chamber in which a partial vacuum can be created.
Claim 17
Dependent Claim
17 words
The apparatus of claim 13 which further includes the body of a syringe attached to the housing.
Claim 18
Dependent Claim
14 words
The apparatus of claim 13 wherein the affinity partner is a specific affinity partner.
Claim 19
Dependent Claim
15 words
The apparatus of claim 13 wherein the affinity partner is an immunoglobulin or derivative thereof.
Claim 20
Dependent Claim
14 words
The apparatus of claim 13 wherein the affinity partner is an antigen or hapten.
Claim 21
Dependent Claim
18 words
The apparatus of claim 16 wherein the immunoglobulin or derivative thereof is an antibody specific for the analyte.
Claim 22
Dependent Claim
15 words
The apparatus of claim 13 wherein the housing supports the membrane in a horizontal position.
Claim 23
Dependent Claim
17 words
The apparatus of claim 13 wherein said housing engages said membrane by means of a deformable lip.
Claim 24
Dependent Claim
17 words
The apparatus of claim 13 wherein the membrane is engaged at the top face of the housing.
Claim 25
Dependent Claim
23 words
The apparatus of claim 13 which further includes an outer chamber disposed at the top of the housing capable of accommodating the sample.
Claim 26
Dependent Claim
73 words
The apparatus of claim 13 wherein said apparatus comprises an upper portion and a lower portion, wherein said lower portion is a luer socket contiguous with a lip portion and wherein said upper portion is a cylindrical chamber having at one end a flange of dimension to engage said lip of the lower portion and wherein said membrane is supported between the flange of the upper portion and lip of the lower portion.
Claim 27
Dependent Claim
68 words
The apparatus of claim 13 wherein said apparatus has an upper portion and a lower portion, wherein said lower portion is a luer socket having at one end an extended rim on which said membrane is supported, said upper portion fitting annularly at the exterior surface of the lower portion and having at the top thereof a lip extension which engages the membrane supported on the lower portion.
Claim 28
Dependent Claim
73 words
The apparatus of claim 22 wherein said apparatus has a top portion and bottom portion, wherein said top portion is a cylindrical chamber containing at its base said membrane, said cylindrical chamber having an engaging means adjacent said membrane for attachment of said top portion to said bottom portion, wherein said bottom portion contains an engaging means for the top portion and a means for attachment of the apparatus to a vacuum manifold.
Claim 29
Independent Claim
105 words
A method to conduct an assay for analyte in a sample, wherein the analyte binds to a first affinity partner and wherein said binding is detected by reaction with a labeled second affinity partner, wherein at least one of said first and second affinity partners is specific for analyte, which method comprises: drawing a volume of said sample by means of a pressure gradient through a porous support containing said labeled second affinity partner and through a membrane in which the first affinity partner is contained into a receiving chamber, wherein said pressure gradient is obtained by creating a partial vacuum in said receiving chamber.
Claim 30
Independent Claim
105 words
A method to conduct an assay for analyte in a sample, wherein the analyte binds to a first affinity partner and wherein said binding is detected by reaction with a labeled second affinity partner, wherein at least one of said first and second affinity partners is specific for analyte, which method comprises: drawing a volume of said sample by means of a pressure gradient through a porous support containing said labeled second affinity partner and through a membrane in which the first affinity partner is contained into a receiving chamber, wherein said pressure gradient is obtained by creating a partial vacuum in said receiving chamber.
Claim 31
Independent Claim
59 words
An apparatus for conducting an assay for analyte in a sample which comprises: a needle; attached to a housing in which is supported a membrane containing an affinity partner for the analyte, which membrane is connected to a receiving chamber which includes a means of applying a pressure gradient through the needle and past the membrane into the chamber.
Claim 32
Independent Claim
59 words
An apparatus for conducting an assay for analyte in a sample which comprises: a needle; attached to a housing in which is supported a membrane containing an affinity partner for the analyte, which membrane is connected to a receiving chamber which includes a means of applying a pressure gradient through the needle and past the membrane into the chamber.
Claim 33
Independent Claim
71 words
An apparatus for conducting an assay for an analyte in a sample which comprises a means for applying a pressure gradient past a membrane in which a first affinity partner for the analyte is contained, into a receiving chamber and having upstream of said membrane a porous support containing a second affinity partner conjugated to label, wherein at least one of said first and second affinity partners is specific for analyte.
Claim 34
Independent Claim
71 words
An apparatus for conducting an assay for an analyte in a sample which comprises a means for applying a pressure gradient past a membrane in which a first affinity partner for the analyte is contained, into a receiving chamber and having upstream of said membrane a porous support containing a second affinity partner conjugated to label, wherein at least one of said first and second affinity partners is specific for analyte.
Claim 35
Dependent Claim
34 words
A kit suitable for conducting measurement of an analyte in a biological sample which reagent kit comprises: the supported membrane unit of claim 13, reagents in separate bottles, and instructions for conducting the assay.
Claim 36
Dependent Claim
10 words
The kit of claim 29 which further includes a syringe
Claim 37
Dependent Claim
11 words
The kit of claim 29 which further includes a vacuum manifold.
Claim 38
Dependent Claim
10 words
The kit of claim 30 wherein the syringe is reusable.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-10-16 | 1993-04-17 | 1993-10-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-10-16 | 1997-04-17 | 1997-10-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-10-16 | 2001-04-17 | 2001-10-17 | $7700.00 | Window Closed / Lapsed |