US4868130A
Expired / Lapsed
US
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Methods for conducting specific binding assays
Assignee: Roche Diagnostics Operations Inc
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Filing: 1987-02-20
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Granted: 1989-09-19
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Expires: 2007-02-20
Abstract
Abstract Methods and devices for separating bound label from unbound label within an assay mixture and for predispensing assay reactants in self-contained assay vessels, as well as a method for detecting the presence and/or amount of an analyte within a fluid sample, and a reusable detection vessel for use therein and with specific binding assays in general are disclosed. Significant to the separation of bound label from unbound label is the use of a cushion comprising generally one primary layer and in some cases one or more secondary layers.
Inventors
- 👤 William R. Hargreaves
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 22:02
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; B; B01; B01L; B01L3/00; B01L3/50; B01L3/502; B01L3/5021; G01N33/536; G01N33/537; G01N33/5375; Y; Y10; Y10S; Y10S436/00; Y10S436/806; Y10S436/824
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
129 words
A method for separating bound label from unbound label within an assay mixture formed within an assay vessel, wherein said assay mixture includes one or more binding components, label bound to at least some of said binding components, and a substantially aqueous solution containing unbound label, said binding components differing in apparent density from said aqueous solution, comprising: contacting a primary layer with an assay mixture formed within an assay vessel, said primary layer being immiscible with both said unbound label and said binding components, and of different density than said binding components; and subjecting said assay mixture to conditions sufficient to cause said binding components and said unbound label to separate, based upon the comparative density of said binding components and the aqueous solution containing said unbound label.
Claim 2
Independent Claim
129 words
A method for separating bound label from unbound label within an assay mixture formed within an assay vessel, wherein said assay mixture includes one or more binding components, label bound to at least some of said binding components, and a substantially aqueous solution containing unbound label, said binding components differing in apparent density from said aqueous solution, comprising: contacting a primary layer with an assay mixture formed within an assay vessel, said primary layer being immiscible with both said unbound label and said binding components, and of different density than said binding components; and subjecting said assay mixture to conditions sufficient to cause said binding components and said unbound label to separate, based upon the comparative density of said binding components and the aqueous solution containing said unbound label.
Claim 3
Independent Claim
13 words
The method of claim I wherein said primary layer is immiscible with water.
Claim 4
Dependent Claim
12 words
The method of claim 1 wherein said primary layer is selectively liquifiable.
Claim 5
Dependent Claim
24 words
The method of claim 1 wherein said primary layer is a mixture of two or more water-immiscible substances that are miscible with one another.
Claim 6
Dependent Claim
16 words
The method of claim 4 wherein at least one of said water-immiscible substances is selectively liquifiable.
Claim 7
Dependent Claim
33 words
The method of claim 1 wherein said primary layer is a mixture of two or more water-immiscible substances, at least one of which has a greater density than water when in liquid form.
Claim 8
Dependent Claim
41 words
The method of claim 1 wherein said assay vessel includes a barrier layer, the method including, prior to the step of contacting, contacting said barrier layer with the essay mixture and selectively allowing said assay mixture to contact said primary layer.
Claim 9
Independent Claim
120 words
A method for separating bound label from unbound label within an assay mixture, wherein said assay mixture includes one or more binding components, non-isotopic label bound to at least some of said binding components, and a substantially aqueous solution containing unbound label, said binding components differing in apparent density from said aqueous solution, comprising: contacting a primary layer with an assay mixture, said primary layer being immiscible with both said bound label and said binding components, and of differing density than said binding components; and subjecting said assay mixture to conditions sufficient to cause said binding components and said unbound label to separate, based upon the comparative density of said binding components and the aqueous solution containing said unbound label.
Claim 10
Independent Claim
120 words
A method for separating bound label from unbound label within an assay mixture, wherein said assay mixture includes one or more binding components, non-isotopic label bound to at least some of said binding components, and a substantially aqueous solution containing unbound label, said binding components differing in apparent density from said aqueous solution, comprising: contacting a primary layer with an assay mixture, said primary layer being immiscible with both said bound label and said binding components, and of differing density than said binding components; and subjecting said assay mixture to conditions sufficient to cause said binding components and said unbound label to separate, based upon the comparative density of said binding components and the aqueous solution containing said unbound label.
Claim 11
Independent Claim
147 words
A method for detecting the presence or amount of an analyte within a fluid sample, comprising: combining said fluid sample with a reaction mixture to form an assay mixture, said assay mixture formed within an assay vessel, wherein said assay mixture contains one or more binding components and label, at least some of said label and some of said analyte binding, directly or indirectly, to said binding components; contacting said assay mixture with a primary layer, said binding components and unbound label being immiscible with and of different density than said primary layer; subjecting said assay mixture to conditions sufficient to cause said binding components and said unbound label to separate, based upon the comparative density of said binding components and the assay mixture containing said unbound label; and detecting the label bound to said binding components and therefrom determining the presence or amount of said analyte.
Claim 12
Independent Claim
147 words
A method for detecting the presence or amount of an analyte within a fluid sample, comprising: combining said fluid sample with a reaction mixture to form an assay mixture, said assay mixture formed within an assay vessel, wherein said assay mixture contains one or more binding components and label, at least some of said label and some of said analyte binding, directly or indirectly, to said binding components; contacting said assay mixture with a primary layer, said binding components and unbound label being immiscible with and of different density than said primary layer; subjecting said assay mixture to conditions sufficient to cause said binding components and said unbound label to separate, based upon the comparative density of said binding components and the assay mixture containing said unbound label; and detecting the label bound to said binding components and therefrom determining the presence or amount of said analyte.
Claim 13
Dependent Claim
12 words
The method of claim 9 wherein said primary layer is selectively liquifiable.
Claim 14
Dependent Claim
25 words
The method of claim 9 wherein said primary layer is a mixture of two or more water-immiscible, dense oils that are miscible with one another.
Claim 15
Dependent Claim
16 words
The method of claim 11 wherein at least one of said water-immiscible substances is selectively liquifiable.
Claim 16
Dependent Claim
33 words
The method of claim 9 wherein said primary layer is a mixture of two or more water-immiscible substances, at least one of which has a greater density than water when in liquid form.
Claim 17
Dependent Claim
16 words
The method of claim 9 wherein said binding component is a magnetic or magnetizable binding component.
Claim 18
Independent Claim
140 words
A method for detecting the presence or amount of an analyte within a fluid sample, comprising: combining said fluid sample with a reaction mixture to form an assay mixture, wherein said assay mixture contains one or more binding components and non-isotopic label, at least some of said label and some of said analyte binding, directly or indirectly, to said binding components; contacting said assay mixture with a primary layer, said binding components and unbound label being immiscible with and of different density than said primary layer; subjecting said assay mixture to conditions sufficient to cause said binding components and said unbound label to separate, based upon the comparative density of said binding components and the assay mixture containing said unbound label; and detecting the label bound to said binding components and therefrom determining the presence or amount of said analyte.
Claim 19
Independent Claim
140 words
A method for detecting the presence or amount of an analyte within a fluid sample, comprising: combining said fluid sample with a reaction mixture to form an assay mixture, wherein said assay mixture contains one or more binding components and non-isotopic label, at least some of said label and some of said analyte binding, directly or indirectly, to said binding components; contacting said assay mixture with a primary layer, said binding components and unbound label being immiscible with and of different density than said primary layer; subjecting said assay mixture to conditions sufficient to cause said binding components and said unbound label to separate, based upon the comparative density of said binding components and the assay mixture containing said unbound label; and detecting the label bound to said binding components and therefrom determining the presence or amount of said analyte.
Claim 20
Dependent Claim
16 words
The method of claim 15 wherein said binding component is a magnetic or magnetizable binding component.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
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| 7.5 Years (Year 8) | 1996-09-18 | 1997-03-20 | 1997-09-19 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-09-18 | 2001-03-20 | 2001-09-19 | $7700.00 | Window Closed / Lapsed |