US4868113A
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Recombinant DNA vector encoding human endothelial cell growth factor
Assignee: Gencell Inc
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Filing: 1986-03-03
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Granted: 1989-09-19
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Expires: 2006-03-03
Abstract
Abstract Endothelial cell growth factor is achieved through the application of recombinant DNA technology to prepare cloning vehicles encoding the ECGF protein and procedures are disclosed for recovering ECGF protein essentially free of other proteins of human origin. The product is useful for, among other purposes, diagnostic applications and as potential in the treatment of damaged blood vessels or other endothelial cell-line structures.
Inventors
- 👤 Michael Jaye
- 👤 Wilson Burgess
- 👤 Thomas Maciag
- 👤 William Drohan
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 21:33
CPC Classifications
C; C12; C12P; C12P21/00; C07; C07K; C07K14/00; C07K14/435; C07K14/475; C07K14/50; C07K14/501; A; A61; A61P; A61P17/00; A61P43/00; C12P21/02; A61K; A61K38/00; Y; Y10; Y10S; Y10S930/00; Y10S930/01; Y10S930/12
Claims Analysis
Total Claims: 27
Claim 1
Independent Claim
60 words
A process for producing a human endothelial cell mitogenic protein comprising, providing a replicable DNA expression vector capable of expressing a DNA sequence encoding human endothelial cell growth factor in a suitable host, transforming said host to obtain a recombinant host, and maintaining said recombinant host under conditions permitting expression of said DNA sequences to produce endothelial cell growth factor.
Claim 2
Dependent Claim
60 words
A process for producing a human endothelial cell mitogenic protein comprising, providing a replicable DNA expression vector capable of expressing a DNA sequence encoding human endothelial cell growth factor in a suitable host, transforming said host to obtain a recombinant host, and maintaining said recombinant host under conditions permitting expression of said DNA sequences to produce endothelial cell growth factor.
Claim 3
Dependent Claim
40 words
The process according to claim 1 wherein recovering said endothelial cell growth factor comprises reaction of the proteins expressed by the recombinant host system with a reagent composition comprising at least one binding protein specific for endothelial cell growth factor.
Claim 4
Dependent Claim
42 words
An isolated DNA sequence consisting essentially of a DNA sequence encoding human beta endothelial cell growth factor including an initiation codon positioned upstream and adjacent to the codon encoding the NH2-terminal amino acid of naturally occurring human beta endothelial cell growth factor.
Claim 5
Dependent Claim
42 words
An isolated DNA sequence consisting essentially of a DNA sequence encoding human beta endothelial cell growth factor including an initiation codon positioned upstream and adjacent to the codon encoding the NH2-terminal amino acid of naturally occurring human beta endothelial cell growth factor.
Claim 6
Dependent Claim
39 words
An isolated DNA sequence encoding a human endothelial cell mitogenic protein, consisting essentially of an initiation codon positioned upstream and adjacent to an open reading frame consisting essentially of a DNA sequence encoding human alpha endothelial cell growth factor.
Claim 7
Dependent Claim
39 words
An isolated DNA sequence encoding a human endothelial cell mitogenic protein, consisting essentially of an initiation codon positioned upstream and adjacent to an open reading frame consisting essentially of a DNA sequence encoding human alpha endothelial cell growth factor.
Claim 8
Dependent Claim
31 words
An isolated DNA sequence according to claim 4 wherein the 5'-terminus of said open reading frame begins with 5'-AAT TAC AAG CCC AAA CTC CTC TAC or an equivalent codon series.
Claim 9
Dependent Claim
30 words
An isolated DNA sequence according to claim 4 wherein the 5'-terminus of said open reading frame begins with 5'-TTT AAT CTG CCT GGG AAT TAC or an equivalent codon series.
Claim 10
Dependent Claim
13 words
A vector comprising a DNA sequence according to claim 4, 5 or 6.
Claim 11
Dependent Claim
17 words
A recombinant host microorganism including the plasmid comprising a DNA sequence to claim 4, 5 or 6.
Claim 12
Dependent Claim
31 words
A recombinant host microorganism containing a DNA expression vector comprising a DNA sequence consisting essentially of a DNA sequence encoding alpha endothelial cell growth factor and capable of expressing said factor.
Claim 13
Dependent Claim
31 words
A recombinant host microorganism containing a DNA expression vector comprising a DNA sequence consisting essentially of a DNA sequence encoding alpha endothelial cell growth factor and capable of expressing said factor.
Claim 14
Dependent Claim
22 words
A replicable DNA expression vector capable of expressing a DNA sequence according to claim 4 or 5 in a self-replicating recombinant system.
Claim 15
Dependent Claim
11 words
The self-replicating recombinant system transformed with the vector of claim 10.
Claim 16
Dependent Claim
14 words
The recombinant system according to claim 11 wherein said system is in a cell.
Claim 17
Dependent Claim
12 words
The recombinant system according to claim 11 wherein said system is cell-free.
Claim 18
Dependent Claim
29 words
The recombinant system according to claim 11 obtained by transforming or infecting a member of the group consisting of an E. coli, B. subtilis, insect, yeast and vertebrate cell.
Claim 19
Dependent Claim
11 words
The recombinant system according to claim 11 obtained by transforming eukaryotes.
Claim 20
Dependent Claim
14 words
A cDNA clone comprising a DNA sequence coding alpha human endothelial cell growth factor.
Claim 21
Dependent Claim
14 words
A cDNA clone comprising a DNA sequence coding alpha human endothelial cell growth factor.
Claim 22
Dependent Claim
35 words
A cDNA clone according to claim 16 containing untranslated nucleotide sequences located 3, to the coding sequence for human alpha endothelial cell growth factor as defined by the human beta endothelial cell growth factor mRNA.
Claim 23
Dependent Claim
17 words
A process according to claim 1 wherein said growth factor is human alpha endothelial cell growth factor.
Claim 24
Dependent Claim
17 words
A process according to claim 1 wherein said growth factor is human beta endothelial cell growth factor.
Claim 25
Dependent Claim
50 words
A process for producing a purified human endothelial cell mitogenic protein comprising maintaining a recombinant host microorganism, which includes a plasmid capable of expressing the open reading frame of the DNA sequence according to claim 4, under conditions such that said microorganism produces said mitogenic protein, and isolating said protein.
Claim 26
Dependent Claim
30 words
A process according to claim 20 wherein the 5,-terminus of said open reading frame begins with 5'-AAT TAC AAG AAG CCC AAA CTC CTC TAC or an equivalent codon series.
Claim 27
Dependent Claim
31 words
A process according to claim 21 wherein said isolation comprises eluting a column formed from crude protein derived from said microorganism with hydrated heparin-Sepharose and collecting said protein in purified form.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-09-18 | 1993-03-20 | 1993-09-19 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-09-18 | 1997-03-20 | 1997-09-19 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-09-18 | 2001-03-20 | 2001-09-19 | $7700.00 | Window Closed / Lapsed |