US4865995A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method for measuring a concentration of saccharides having a low molecular weight and sensor for measuring the same
Assignee: Kanegafuchi Chemical Industry Co Ltd
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Filing: 1986-02-04
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Granted: 1989-09-12
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Expires: 2006-02-04
Abstract
Abstract A method for measuring the concentration of low molecular weight saccharides in a liquid by placing the liquid in contact with one side of a membrane which allows saccharides to pass through, but not polysaccharides. A detecting liquid containing polysaccharides is placed in contact with the other side of the membrane. The detecting liquid is also in contact with a ligand which has a higher affinity for the saccharides than for the polysaccharides. The concentration of saccharides is calculated from the measured change in the concentration of polysaccharides in the detecting liquid. A sensor for measuring the concentration of low molecular weight saccharides based on the same method is also described.
Inventors
- 👤 Kazuo Dairaku
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 13:44
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/66; C; C12; C12M; C12M41/00; C12M41/30; C12M41/32; G01N21/00; G01N21/17; G01N21/25; G01N21/31; G01N21/35; G01N21/3577; G01N30/00; G01N30/02; Y; Y10; Y10T; Y10T436/00; Y10T436/14; Y10T436/142222; Y10T436/143333; Y10T436/144444; Y10T436/25; Y10T436/25375; Y10T436/255
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
240 words
A method for measuring the concentration of saccharides having a low molecular weights dissolved in an aqueous medium, said method comprising the steps of: (a) bringing a liquid to be analyzed in contact with one side of a membrane provided inside a permeation member, and bringing a detecting liquid in contact with the other side of said membrane inside said permeation member, wherein the detecting liquid contains an aqueous solution of blue dextran, and said membrane allows said saccharides having a low molecular weights to pass through, but does not allow blue dextran to pass through; (b) circulating said detecting liquid from the permeation member to come into contact with a ligand provided inside an adsorption-desorption member, wherein said ligand has an affinity for both the saccharides having a low molecular weight and blue dextran, said affinity for the saccharides having a low molecular weight being greater than said affinity for blue dextran; (c) measuring the concentration of blue dextran in the detecting liquid by measuring the absorption of light of a selected wavelength by the detecting; and (d) calculating the concentration of saccharides having a low molecular weight in the liquid to be analyzed from the change in the concentration of blue dextran in the detecting liquid, said change being due to the adsorption or desorption of said saccharides having a low molecular weight by the ligand and the corresponding desorption or adsorption of blue dextran by the ligand.
Claim 2
Independent Claim
240 words
A method for measuring the concentration of saccharides having a low molecular weights dissolved in an aqueous medium, said method comprising the steps of: (a) bringing a liquid to be analyzed in contact with one side of a membrane provided inside a permeation member, and bringing a detecting liquid in contact with the other side of said membrane inside said permeation member, wherein the detecting liquid contains an aqueous solution of blue dextran, and said membrane allows said saccharides having a low molecular weights to pass through, but does not allow blue dextran to pass through; (b) circulating said detecting liquid from the permeation member to come into contact with a ligand provided inside an adsorption-desorption member, wherein said ligand has an affinity for both the saccharides having a low molecular weight and blue dextran, said affinity for the saccharides having a low molecular weight being greater than said affinity for blue dextran; (c) measuring the concentration of blue dextran in the detecting liquid by measuring the absorption of light of a selected wavelength by the detecting; and (d) calculating the concentration of saccharides having a low molecular weight in the liquid to be analyzed from the change in the concentration of blue dextran in the detecting liquid, said change being due to the adsorption or desorption of said saccharides having a low molecular weight by the ligand and the corresponding desorption or adsorption of blue dextran by the ligand.
Claim 3
Dependent Claim
27 words
The method of claim 1, wherein the saccharides having a low molecular weight are those having a mean molecular weight of not more than 1×10 3 .
Claim 4
Dependent Claim
22 words
The method of claim 1 or 2, wherein the polysaccharides have a mean molecular weight of not less than 5×10 3 .
Claim 5
Dependent Claim
24 words
The method of claim 3, wherein the polysaccharides have a mean molecular weight in the range of from 1×10 4 to 5×10 6 .
Claim 6
Dependent Claim
10 words
The method of claim 1, wherein the ligand is lectin.
Claim 7
Dependent Claim
12 words
The method of claim 1, wherein the membrane is an ultrafiltration membrane.
Claim 8
Dependent Claim
11 words
The method of claim 1, wherein the membrane is a dialyzer.
Claim 9
Dependent Claim
190 words
A circulation-type sensor for measuring the concentration of saccharides having a low molecular weight dissolved in an aqueous medium, said sensor comprising: (a) a permeation member; (b) a membrane provided inside said permeation member; (c) a detecting liquid disposed inside said permeation member on one side of said membrane and containing an aqueous solution of blue dextran, wherein said membrane allows said saccharides having a low molecular weight to pass through, but does not allow blue dextran to pass through; (d) means for holding a liquid to be analyzed in contact with the other side of said membrane inside said permeation member; (e) an adsorption--desorption member comprising a ligand, said ligand having an affinity for both the saccharides having a low molecular weight and blue dextran, said affinity for the saccharides having a low molecular weights being greater than said affinity for blue dextran; (f) means for continuously circulating and conducting the detecting liquid between said permeation member and said adsorption--desorption member; and (g) means for measuring the concentration of blue dextran in the detecting liquid by measuring the absorption of light of a selected wavelength by the detecting liquid.
Claim 10
Dependent Claim
190 words
A circulation-type sensor for measuring the concentration of saccharides having a low molecular weight dissolved in an aqueous medium, said sensor comprising: (a) a permeation member; (b) a membrane provided inside said permeation member; (c) a detecting liquid disposed inside said permeation member on one side of said membrane and containing an aqueous solution of blue dextran, wherein said membrane allows said saccharides having a low molecular weight to pass through, but does not allow blue dextran to pass through; (d) means for holding a liquid to be analyzed in contact with the other side of said membrane inside said permeation member; (e) an adsorption--desorption member comprising a ligand, said ligand having an affinity for both the saccharides having a low molecular weight and blue dextran, said affinity for the saccharides having a low molecular weights being greater than said affinity for blue dextran; (f) means for continuously circulating and conducting the detecting liquid between said permeation member and said adsorption--desorption member; and (g) means for measuring the concentration of blue dextran in the detecting liquid by measuring the absorption of light of a selected wavelength by the detecting liquid.
Claim 11
Dependent Claim
18 words
The sensor of claim 8, wherein the permeation member and the adsorption-desorption member are united in one piece.
Claim 12
Dependent Claim
22 words
The sensor of claim 8, wherein the detecting liquid further contains an aqueous solution of said saccharides having a low molecular weight.
Claim 13
Dependent Claim
12 words
The sensor of claim 8, wherein said adsorption-desorption member is a column.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-09-11 | 1993-03-13 | 1993-09-12 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-09-11 | 1997-03-13 | 1997-09-12 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-09-11 | 2001-03-13 | 2001-09-12 | $7700.00 | Window Closed / Lapsed |