US4865733A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Cell separator device
Assignee: Pentax Corp
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Filing: 1987-03-20
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Granted: 1989-09-12
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Expires: 2007-03-20
Abstract
Abstract A cell separator which can separate cells, especially lymphocytes, with high selectivity and high efficiency without changing the cell population ratio of the cells. The cell separator comprises fibrous material containing fibers including hydroxyl apatite.
Inventors
- 👤 Sumiaki Tsuru
- 👤 Shoichi Mori
- 👤 Noriko Komatsu
- 👤 Shigeo Fujii
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 08:04
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; C; C12; C12Q; C12Q1/00; C12Q1/02; C12Q1/24; B; B01; B01J; B01J20/00; B01J20/02; B01J20/04; B01J20/048; B01J20/28; B01J20/28002; B01J20/28004; B01J20/28014; B01J20/28028; B01J20/2803; B01J20/28052; B01J20/30; B01J20/3042; B01J20/3078
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
53 words
A device for separating cells with high selectivity and reproducibility and without substantially changing the cell population, which comprises: a separating column; fibers containing hydroxyl apatite disposed in said separating column; inlet means for introducing cells to be separated into said separating column; and outlet means for removing cells from said separating column.
Claim 2
Independent Claim
53 words
A device for separating cells with high selectivity and reproducibility and without substantially changing the cell population, which comprises: a separating column; fibers containing hydroxyl apatite disposed in said separating column; inlet means for introducing cells to be separated into said separating column; and outlet means for removing cells from said separating column.
Claim 3
Dependent Claim
21 words
The device of claim 1 wherein the content of the hydroxyl apatite in the fiber is 25 to 50% by weight.
Claim 4
Dependent Claim
14 words
The device of claim 1 wherein a reinforcing material is incorporated into the fiber.
Claim 5
Dependent Claim
27 words
The device of claim 3 wherein the reinforcing material is calcium phosphate and water glass which is present in an amount of up to 25% by weight.
Claim 6
Dependent Claim
20 words
The device of claim 1 wherein the cell separator contains water in an amount of up to 50% by weight.
Claim 7
Dependent Claim
28 words
The device of claim 1 wherein the hydroxyl apatite is Ca 5 (PO 4 ) 3 (OH) in which the molar ratio of Ca/P is 1.4 to 1.8.
Claim 8
Dependent Claim
16 words
The device of claim 1 wherein the diameter of the fiber is 1 to 100 μm.
Claim 9
Dependent Claim
22 words
The device of claim 1 wherein the weight of the fibrous cell separator is 5 g/m 2 to 500 g/m 2 .
Claim 10
Dependent Claim
14 words
The device of claim 1 wherein the column further contains granules of hydroxly apatite.
Claim 11
Independent Claim
59 words
A device for separating cells with high selectivity and reproducibility and without substantially changing the cells population which comprises a column, inlet means for introducing cells to be separated into said column; outlet means for removing cells from said column, a filter disposed at the lower portion of the column, and fibers containing hydroxyl apatite disposed on said filter.
Claim 12
Independent Claim
59 words
A device for separating cells with high selectivity and reproducibility and without substantially changing the cells population which comprises a column, inlet means for introducing cells to be separated into said column; outlet means for removing cells from said column, a filter disposed at the lower portion of the column, and fibers containing hydroxyl apatite disposed on said filter.
Claim 13
Dependent Claim
20 words
The device of claim 10 wherein hydroxyl apatite granules are placed on the filter and under the fibrous bydroxyl apatite.
Claim 14
Dependent Claim
19 words
The device of claim 11 wherein the average diameter of the hydroxyl apatite granules is 1 to 2000 μm.
Claim 15
Dependent Claim
18 words
The device of claim 11 wherein the granules are sintered at a temperature of 500° to 1,4000° C.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-09-11 | 1993-03-13 | 1993-09-12 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-09-11 | 1997-03-13 | 1997-09-12 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-09-11 | 2001-03-13 | 2001-09-12 | $7700.00 | Window Closed / Lapsed |