In-vitro methods for identifying compositions which are agonists and antagonists of estrogens

Claim 2

An in-vitro method for identifying a substance of interest as an in-vivo estrogen agonist, said method comprising the steps of: obtaining a plurality of cells cultured in-vitro, said cells being estrogen dependent for proliferation in-vivo; maintaining a known quantity of said cultured cells in a medium without estrogens comprising a fluid and an inhibitor of cell proliferation endogenous to the serum of adult humans, said endogenous inhibitor being present in said maintaining medium at a concentration effective to prevent proliferation of said cells in-vitro in the absence of an estrogen; adding the substance of interest to said cultured cells in said maintaining medium to form a reaction mixture; incubating said reaction mixture for a preselected time period; and determining the number of cultured cells in said incubated reaction mixture, a measurable increase in the number of cultured cells identifying the substance of interest as an estrogen agonist.

Claim 3

An in-vitro method for identifying a substance of interest as an in-vivo estrogen antagonist, said method comprising the steps of: obtaining a plurality of cells cultured in-vitro, said cells being estrogen dependent for proliferation in-vivo; maintaining a known quantity of said cultured cells in a medium without estrogens comprising a fluid and an inhibitor of cell proliferation endogenous to the serum of adult humans, said endogenous inhibitor being present in said maintaining medium at a concentration effective to prevent proliferation of said cells in-vitro in the absence of an estrogen; adding the substance of interest and an estrogen to said cultured cells in said maintaining medium to form a reaction mixture; incubating said reaction mixture for a preselected time period; and determining the number of cultured cells in said incubated reaction mixture, the failure of said cultured cells to measurably increase in number identifying the substance of interest as an estrogen antagonist.

Claim 4

An in-vitro method for identifying a substance of interest as an in-vivo estrogen antagonist, said method comprising the steps of: obtaining a plurality of cells cultured in-vitro, said cells being estrogen dependent for proliferation in-vivo; maintaining a known quantity of said cultured cells in a medium without estrogens comprising a fluid and an inhibitor of cell proliferation endogenous to the serum of adult humans, said endogenous inhibitor being present in said maintaining medium at a concentration effective to prevent proliferation of said cells in-vitro in the absence of an estrogen; adding the substance of interest and an estrogen to said cultured cells in said maintaining medium to form a reaction mixture; incubating said reaction mixture for a preselected time period; and determining the number of cultured cells in said incubated reaction mixture, the failure of said cultured cells to measurably increase in number identifying the substance of interest as an estrogen antagonist.

Claim 5

An in-vitro method for identifying a substance of interest as having in-vivo estrogen agonistic and estrogen antagonistic properties, said method comprising the steps of: obtaining a plurality of cells cultured in-vitro, said cells being estrogen dependent for proliferation in-vivo; maintaining a known quantity of said cultured cells in a medium without estrogens comprising a fluid and an inhibitor of cell proliferation endogenous to the serum of adult humans, said endogenous inhibitor being present in said maintaining medium at a concentration effective to prevent proliferation of said cells in-vitro in the absence of an estrogen; adding the substance of interest to a first aliquot of said cultured cells in said maintaining medium to form a first reaction mixture; adding the substance of interest and an estrogen to a second aliquot of said cultured cells in said maintaining medium to form a second reaction mixture; incubating said first reaction mixture and said second reaction mixture for a preselected time period; determining the number of cultured cells in said incubated first reaction mixture, a measurable increase in the number of cultured cells identifying the substance of interest as an estrogen agonist; and determining the number of cultured cells in said incubated second reaction mixture, the failure of said cultured cells to measurably increase in number identifying the substance of interest as an estrogen antagonist.

Claim 6

An in-vitro method for identifying a substance of interest as having in-vivo estrogen agonistic and estrogen antagonistic properties, said method comprising the steps of: obtaining a plurality of cells cultured in-vitro, said cells being estrogen dependent for proliferation in-vivo; maintaining a known quantity of said cultured cells in a medium without estrogens comprising a fluid and an inhibitor of cell proliferation endogenous to the serum of adult humans, said endogenous inhibitor being present in said maintaining medium at a concentration effective to prevent proliferation of said cells in-vitro in the absence of an estrogen; adding the substance of interest to a first aliquot of said cultured cells in said maintaining medium to form a first reaction mixture; adding the substance of interest and an estrogen to a second aliquot of said cultured cells in said maintaining medium to form a second reaction mixture; incubating said first reaction mixture and said second reaction mixture for a preselected time period; determining the number of cultured cells in said incubated first reaction mixture, a measurable increase in the number of cultured cells identifying the substance of interest as an estrogen agonist; and determining the number of cultured cells in said incubated second reaction mixture, the failure of said cultured cells to measurably increase in number identifying the substance of interest as an estrogen antagonist.

Claim 7

The in-vitro method as recited in claim 1, 2, or 3 wherein said cultured cells comprise tumor cells derived from the female human breast.

Claim 8

The in-vitro method as recited in claim 4 wherein said cultured cells are C7 MCF7-173 cells, having ATCC accession number CRL 9836.

Claim 9

The in-vitro method as recited in claim 2 or 3 wherein said estrogen is selected from the group consisting of estrone, estradiol, and estriol.

Claim 10

The in-vitro method as recited in claim 1, 2, or 3 wherein said maintaining medium comprises fetal bovine serum.

Claim 11

The in-vitro method as recited in claim 1, 2, or 3 wherein said maintaining medium comprises Dulbecco's modification of Eagle's Minimal Essential Medium.

Claim 12

The in-vitro method as recited in claim 1, 2, or 3 wherein said maintaining medium comprises charcoal-dextran stripped human serum.

Claim 13

The in-vitro method as recited in claim 9 wherein said serum is used in a concentration ranging from 40-10% concentration by volume.

Claim 14

The in-vitro method as recited in claim 1, 2, or 3 wherein said wherein said inhibitor is added to said maintaining medium as a incompletely proteinaceous preparation.

Claim 15

The in-vitro method as recited in claim 1, 2, or 3 wherein said reaction mixtures are incubated for a time period ranging from 9-14 calendar days.

Claim 16

The in-vitro method as recited in claim 1, 2, or 3 wherein said fluid of said maintaining medium comprises at least one selected from the group consisting of buffers, amino acids, sugars, vitamins, and ionizable salts.