US4857467A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Carbon and energy source markers for transformation of strains of the genes Pichia
Assignee: Research Corp Technologies Inc
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Filing: 1986-07-23
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Granted: 1989-08-15
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Expires: 2006-07-23
Abstract
Abstract Yeast strains are transformed with, and selected for, using DNA fragments encoding a gene function not present in the wild-type yeast strains. The invention requires transforming the yeast with DNA fragments which comprise the gene function which the wild-type yeast strain is lacking; regeneration of the transformants on a non-selective regeneration medium; then selection of those colonies which are capable of growth on a carbon and energy source which requires expression of the gene function provided by the DNA fragment. Thus, transformation with such DNA fragments allows for positive selection of transformed strains.
Inventors
- 👤 Kotikanyadan Sreekrishna
- 👤 Motohiro Fuke
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 23:11
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/24; C12N9/2402; C12N9/2405; C12N9/2408; C12N15/00; C12N15/09; C12N15/63; C12N15/65; C12N15/79; C12N15/80; C12N15/81; C12N15/815; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/911; Y10S435/938
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
8 words
The pure culture comprising Pichia pastoris NRRL y-11430/pTSUI.
Claim 2
Dependent Claim
8 words
The pure culture comprising Pichia pastoris NRRL y-11430/pTSUI.
Claim 3
Dependent Claim
7 words
The pure culture comprising Pichia pastoris y-11430/pSR11.
Claim 4
Dependent Claim
7 words
The pure culture comprising Pichia pastoris y-11430/pSR11.
Claim 5
Dependent Claim
8 words
The pure culture comprising Pichia pastoris NRRL y-15851/pSR1.
Claim 6
Dependent Claim
8 words
The pure culture comprising Pichia pastoris NRRL y-15851/pSR1.
References Cited (Backward Citations)
US4617274A
Cited
GB2125047A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-08-14 | 1993-02-13 | 1993-08-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-08-14 | 1997-02-13 | 1997-08-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-08-14 | 2001-02-13 | 2001-08-15 | $7700.00 | Window Closed / Lapsed |