US4857464A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Mist cultivation of cells
Assignee: Bio Rational Technologies Inc
•
Filing: 1986-02-21
•
Granted: 1989-08-15
•
Expires: 2006-02-21
Abstract
Abstract Cells are cultivated in a chamber having a high nutrient humidity. Gas drives liquid nutrient through devices which produce a fine mist within the chamber. Screens or convoluted mesh supports the cells, and provides for drainage of cell products and media away from the cells for processing. The chamber is sealable to provide for axenic conditions.
Inventors
- 👤 Pamela Weathers
- 👤 Kenneth Giles
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 09:40
CPC Classifications
C; C12; C12M; C12M29/00; C12M29/26; C12M21/00; C12M21/08; C12M23/00; C12M23/24; C12M29/06; C12M29/14; C12M41/00; C12M41/30; C12M41/34
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
68 words
A method of cultivating cells, said cells originating from plants, animals and microbes in both a differentiated and undifferentiated state, comprising: (a) supporting the cells on a liquid and gas permeable member; (b) supporting the member within a sealable container; (c) misting a liquid nutrient with droplets having a droplet size of between about 0.1 and about 10 microns into the chamber to provide a nutrient humid environment.
Claim 2
Independent Claim
68 words
A method of cultivating cells, said cells originating from plants, animals and microbes in both a differentiated and undifferentiated state, comprising: (a) supporting the cells on a liquid and gas permeable member; (b) supporting the member within a sealable container; (c) misting a liquid nutrient with droplets having a droplet size of between about 0.1 and about 10 microns into the chamber to provide a nutrient humid environment.
Claim 3
Dependent Claim
17 words
Method of claim 1, further comprising the step of: (d) introducing a gas into the sealable container.
Claim 4
Dependent Claim
22 words
Method of claim 2, further comprising the step of: (e) controlling entry of gas and nutrients with a processor and associated hardware.
Claim 5
Dependent Claim
21 words
Method of claim 1, wherein in step (a) the liquid and gas permeable member is a screen of biologically inert material.
Claim 6
Dependent Claim
22 words
Method of claim 1, wherein in step (a) the liquid and gas permeable member is a convoluted mesh of biologically inert material.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-08-14 | 1993-02-13 | 1993-08-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-08-14 | 1997-02-13 | 1997-08-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-08-14 | 2001-02-13 | 2001-08-15 | $7700.00 | Window Closed / Lapsed |