Optical pulse particle size analyzer

Claim 1

A system for determining the cluster size distribution of submicron-size particles suspended in a liquid, comprising: a. a laminar flow cell for passage of a sheath liquid and a sample liquid containing suspended, clustered particles, the flow cell having a translucent chamber configured to constrain the suspended particles to passage in single file through the chamber; b. means for passing the sheath liquid through the chamber at a continuous flow rate; c. infusion pump means for passing the sample liquid into the sheath liquid stream and through the chamber at a continuous rate of flow; d. a light source for generating a beam of light; e. focusing means for focusing the beam of light onto the translucent chamber of the flow cell such that the beam is substantially perpendicular to the direction of particle flow through the chamber, wherein the focusing means comprises one or more cylindrical lenses which constricts the light beam to an elliptical shape such that the direction of flow of the particles through the chamber is along the minor axis of the ellipse; f. a collecting lens for collecting pulses of light scattered from the particles in the sample liquid; g. means for limiting the collected light to light scattered from the particles up to 5.2 degrees from the axis of the beam of light; h. a stop, located between the lens and the flow cell in the path of the beam of light transmitted through the flow cell, which stop diverts the transmitted beam of light from its axis; i. an iris, located between the stop and the lens, having a diaphragm of a diameter which excludes light scattered at angles greater than about 3.0-5.2 degrees from the axis of the beam of light; j. light sensing means for sensing the intensity of scattered light collected by the lens and for converting the sensed light pulse to an electrical signal proportional to the intensity of the scattered light pulse; and k. signal processing means for determining the cluster size distribution of particles.

Claim 2

A system for determining the cluster size distribution of submicron-size particles suspended in a liquid, comprising: a. a laminar flow cell for passage of a sheath liquid and a sample liquid containing suspended, clustered particles, the flow cell having a translucent chamber configured to constrain the suspended particles to passage in single file through the chamber; b. means for passing the sheath liquid through the chamber at a continuous flow rate; c. infusion pump means for passing the sample liquid into the sheath liquid stream and through the chamber at a continuous rate of flow; d. a light source for generating a beam of light; e. focusing means for focusing the beam of light onto the translucent chamber of the flow cell such that the beam is substantially perpendicular to the direction of particle flow through the chamber, wherein the focusing means comprises one or more cylindrical lenses which constricts the light beam to an elliptical shape such that the direction of flow of the particles through the chamber is along the minor axis of the ellipse; f. a collecting lens for collecting pulses of light scattered from the particles in the sample liquid; g. means for limiting the collected light to light scattered from the particles up to 5.2 degrees from the axis of the beam of light; h. a stop, located between the lens and the flow cell in the path of the beam of light transmitted through the flow cell, which stop diverts the transmitted beam of light from its axis; i. an iris, located between the stop and the lens, having a diaphragm of a diameter which excludes light scattered at angles greater than about 3.0-5.2 degrees from the axis of the beam of light; j. light sensing means for sensing the intensity of scattered light collected by the lens and for converting the sensed light pulse to an electrical signal proportional to the intensity of the scattered light pulse; and k. signal processing means for determining the cluster size distribution of particles.