US4849362A
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Fluorescent intracellular calcium indicators
Assignee: SmithKline Beecham Corp
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Filing: 1988-05-19
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Granted: 1989-07-18
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Expires: 2008-05-19
Abstract
Abstract Invented are new tetracarboxylate compounds which are chelators for calcium ions. Also invented is a useful method of measuring intracellular calcium concentrations using these novel compounds as an optical indicator.
Inventors
- 👤 Robert M. DeMarinis
- 👤 Haralambos E. Katerinopoulos
- 👤 Katharine A. Muirhead
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 22:04
CPC Classifications
C; C07; C07D; C07D403/00; C07D403/02; C07D403/06; C07D405/00; C07D405/02; C07D405/06; C07D409/00; C07D409/02; C07D409/06; C07D417/00; C07D417/02; C07D417/06; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/84
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
43 words
A compound of the formula: ##STR5## in which: R is hydrogen or acetoxymethyl; R 1 is methyl or ethyl; Z is O, S or NH: and Y is NH or S; or when R is hydrogen, a sodium, potassium or lithium salt thereof.
Claim 2
Dependent Claim
43 words
A compound of the formula: ##STR5## in which: R is hydrogen or acetoxymethyl; R 1 is methyl or ethyl; Z is O, S or NH: and Y is NH or S; or when R is hydrogen, a sodium, potassium or lithium salt thereof.
Claim 3
Dependent Claim
11 words
A compound according to claim 1 in which R is acetoxymethyl.
Claim 4
Dependent Claim
12 words
A compound according to claim 1 in which R 1 is methyl.
Claim 5
Dependent Claim
19 words
A compound according to claim 1 in which R 1 is methyl, Z is 0 and Y is NH.
Claim 6
Dependent Claim
12 words
A compound according to claim 2 in which R 1 is methyl.
Claim 7
Dependent Claim
19 words
A compound according to claim 2 in which R 1 is methyl, Z is O and Y is NH.
Claim 8
Dependent Claim
36 words
A method of determining intracellar calcium concentration in intact cells via an optical measurement system which comprises treating said cells with a sufficient quantity of a compound of claim 1 to act as an optical indicator.
Claim 9
Dependent Claim
20 words
A method of claim 7 in which flow cytometry or quantitative fluorescence microscopy is used to measure intracellular calcium concentrations.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-07-17 | 1993-01-16 | 1993-07-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-07-17 | 1997-01-16 | 1997-07-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-07-17 | 2001-01-16 | 2001-07-18 | $7700.00 | Window Closed / Lapsed |