US4849352A
Expired / Lapsed
US
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Antibody purification process
Assignee: BTG International Inc
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Filing: 1984-10-09
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Granted: 1989-07-18
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Expires: 2004-10-09
Abstract
Abstract F(ab) fragments are isolated from an antibody containing source by contacting the antibody containing source with a papain-polyacrylamide matrix to produce F(ab) and F(c) fragments which are then passed through an antigen-polyacrylamide gel capable of attracting the F(ab) fragments. F(ab) 2 fragments are obtained by contacting the antibody containing source with a pepsin-polyacrylamide matrix to produce F(ab) 2 and F(c) fragments which are then passed through an antigen-polyacrylamide gel capable of attracting the F(ab) 2 fragments. IgG antibodies are obtained by passing an antibody containing source through an antigen-polyacrylamide gel. These processes can be used to purifiy a wide variety of antibodies which can be used as therapeutic agents and as diagnostic agents. Antivenins produced by these processes have substantially reduced foreign protein levels and hence are less likely to produce immunogenic reactions. Bulk, unprocessed antibody sources may be utilized and, for reasons of process simplification, are preferred.
Inventors
- 👤 John B. Sullivan
- 👤 Findlay E. Russell
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 15:21
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/06; C07K16/065; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/5308; A; A61; A61K; A61K38/00; C07K2317/00; C07K2317/50; C07K2317/55; Y; Y10; Y10S; Y10S530/00; Y10S530/866
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
80 words
A process for isolating F(ab) fragments from an antibody containing source comprising: contacting the antibody containing source with a papain-polyacrylamide matrix to obtain a solution containing F(ab) and F(c) fragments; and passing the solution containing the F(ab) and F(c) fragments through an affinity chromatography system having a gel comprised of an antigen having an affinity for the F(ab) fragments and which is embedded in a polyacrylamide matrix, whereby the F(ab) fragments are isolated from the F(c) fragments for subsequent recovery.
Claim 2
Dependent Claim
80 words
A process for isolating F(ab) fragments from an antibody containing source comprising: contacting the antibody containing source with a papain-polyacrylamide matrix to obtain a solution containing F(ab) and F(c) fragments; and passing the solution containing the F(ab) and F(c) fragments through an affinity chromatography system having a gel comprised of an antigen having an affinity for the F(ab) fragments and which is embedded in a polyacrylamide matrix, whereby the F(ab) fragments are isolated from the F(c) fragments for subsequent recovery.
Claim 3
Dependent Claim
16 words
The process of claim 1 wherein the antibody containing source is a bulk, unprocessed hyperimmune serum.
Claim 4
Dependent Claim
15 words
The process of claim 1 wherein the antibody containing source is a monoclonal antibody source.
Claim 5
Dependent Claim
16 words
The process of claim 1 wherein the antibody containing source is partially purified by precipitation procedures.
Claim 6
Dependent Claim
80 words
A process for isolating F(ab) fragments from an antibody containing source comprising: contacting the antibody containing source with a papain-polyacrylamide matrix to obtain a solution containing F(ab) and F(c) fragments; and passing the solution containing the F(ab) and F(c) fragments through an affinity chromatography system having a gel comprised of an antigen having an affinity for the F(c) fragments and which is embedded in a polyacrylamide matrix, whereby the F(ab) fragments are isolated from the F(c) fragments for subsequent recovery.
Claim 7
Dependent Claim
80 words
A process for isolating F(ab) fragments from an antibody containing source comprising: contacting the antibody containing source with a papain-polyacrylamide matrix to obtain a solution containing F(ab) and F(c) fragments; and passing the solution containing the F(ab) and F(c) fragments through an affinity chromatography system having a gel comprised of an antigen having an affinity for the F(c) fragments and which is embedded in a polyacrylamide matrix, whereby the F(ab) fragments are isolated from the F(c) fragments for subsequent recovery.
Claim 8
Dependent Claim
16 words
The process of claim 5 wherein the antibody containing source is a bulk, unprocessed hyperimmune serum.
Claim 9
Dependent Claim
15 words
The process of claim 5 wherein the antibody containing source is a monoclonal antibody source.
Claim 10
Dependent Claim
16 words
The process of claim 5 wherein the antibody containing source is partially purified by precipitation procedures.
Claim 11
Dependent Claim
82 words
A process for isolating F(ab) 2 fragments from an antibody containing source comprising: contacting the antibody containing source with a pepsin-polyacrylamide matrix to obtain a solution containing F(ab) 2 and F(c) fragments; and passing the solution containing the F(ab) 2 and F(c) fragments through an affinity chromatography system having a gel comprised of an antigen having an affinity for the F(ab) 2 fragments embedded in a polyacrylamide matrix, whereby the F(ab) 2 fragments are isolated from the F(c) fragments for subsequent recovery.
Claim 12
Dependent Claim
82 words
A process for isolating F(ab) 2 fragments from an antibody containing source comprising: contacting the antibody containing source with a pepsin-polyacrylamide matrix to obtain a solution containing F(ab) 2 and F(c) fragments; and passing the solution containing the F(ab) 2 and F(c) fragments through an affinity chromatography system having a gel comprised of an antigen having an affinity for the F(ab) 2 fragments embedded in a polyacrylamide matrix, whereby the F(ab) 2 fragments are isolated from the F(c) fragments for subsequent recovery.
Claim 13
Dependent Claim
16 words
The process of claim 9 wherein the antibody containing source is a bulk, unprocessed hyperimmune serum.
Claim 14
Dependent Claim
15 words
The process of claim 9 wherein the antibody containing source is a monoclonal antibody source.
Claim 15
Dependent Claim
16 words
The process of claim 9 wherein the antibody containing source is partially purified by precipitation procedures.
Claim 16
Dependent Claim
82 words
A process for isolating F(ab) 2 fragments from a bulk antibody containing source comprising: contacting the antibody containing source with a pepsin-polyacrylamide matrix to obtain a solution containing F(ab) 2 and F(c) fragments; and passing the solution containing the F(ab) 2 and F(c) fragments through an affinity chromatography system having a gel comprised of an antigen having an affinity for the F(c) fragments embedded in a polyacrylamide matrix, whereby the F(ab) 2 fragments are isolated from the f(c) fragments for subsequent recovery.
Claim 17
Dependent Claim
82 words
A process for isolating F(ab) 2 fragments from a bulk antibody containing source comprising: contacting the antibody containing source with a pepsin-polyacrylamide matrix to obtain a solution containing F(ab) 2 and F(c) fragments; and passing the solution containing the F(ab) 2 and F(c) fragments through an affinity chromatography system having a gel comprised of an antigen having an affinity for the F(c) fragments embedded in a polyacrylamide matrix, whereby the F(ab) 2 fragments are isolated from the f(c) fragments for subsequent recovery.
Claim 18
Dependent Claim
16 words
The process of claim 13 wherein the antibody containing source is a bulk, unprocessed hyperimmune serum.
Claim 19
Dependent Claim
15 words
The process of claim 13 wherein the antibody containing source is a monoclonal antibody source.
Claim 20
Dependent Claim
16 words
The process of claim 13 wherein the antibody containing source is partially purified by precipitation procedures.
References Cited (Backward Citations)
US4012502A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-07-17 | 1993-01-16 | 1993-07-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-07-17 | 1997-01-16 | 1997-07-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-07-17 | 2001-01-16 | 2001-07-18 | $7700.00 | Window Closed / Lapsed |