Patent FR2216638A1 successfully refreshed from Google Patents.
US4847376A
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Lipase substrates
Assignee: Roche Diagnostics GmbH
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Filing: 1986-05-01
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Granted: 1989-07-11
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Expires: 2006-05-01
Abstract
Abstract The present invention provides a lipase substrate of the general formula: <IMAGE> wherein A is an alkylene or alkenylene radical containing up to 16 carbon atoms, R and R1, which can be the same or different, each signify an alkyl, alkenyl or acyl radical containing up to 20 carbon atoms or an optionally alkyl-substituted aryl or aralkyl radical containing up to 8 carbon atoms in the alkyl moiety and wherein one of R and R1 can also be a hydrogen atom, X is the residue of an aromatic hydroxy or thiol compound, and each Y and Z, independently from each other, is -S- or -O-, Z also -CH2-. The present invention also provides a process and a reagent for the optical determination of lipase.
Inventors
- 👤 Ulrich Neumann
- 👤 Martina Junius
- 👤 Hans-Georg Batz
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 19:11
CPC Classifications
C; C07; C07D; C07D265/00; C07D265/28; C07D265/34; C07D265/38; C12; C12Q; C12Q1/00; C12Q1/34; C12Q1/44; C12Q2334/00
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
123 words
A lipase substrate of the formula: ##STR3## wherein A is an alkylene or alkenylene radical containing up to 16 carbon atoms, R and R 1 , which can be the same or different, each signify alkyl, alkenyl or acyl containing up to 20 carbon atoms or an optionally alkyl-substituted aryl or aralkyl radical containing up to 8 carbon atoms in the alkyl moiety and wherein one of R and R 1 can also be a hydrogen atom, X is an aromatic hydroxy or thiol residue selected from the group consisting of resorufin, chlorophenol red, indoxyl, and thiofluorescein each Y independently from each other, is --S-- or --O--, and each Z independently from each other is other is --S--, --O-- or --CH 2 --.
Claim 2
Dependent Claim
123 words
A lipase substrate of the formula: ##STR3## wherein A is an alkylene or alkenylene radical containing up to 16 carbon atoms, R and R 1 , which can be the same or different, each signify alkyl, alkenyl or acyl containing up to 20 carbon atoms or an optionally alkyl-substituted aryl or aralkyl radical containing up to 8 carbon atoms in the alkyl moiety and wherein one of R and R 1 can also be a hydrogen atom, X is an aromatic hydroxy or thiol residue selected from the group consisting of resorufin, chlorophenol red, indoxyl, and thiofluorescein each Y independently from each other, is --S-- or --O--, and each Z independently from each other is other is --S--, --O-- or --CH 2 --.
Claim 3
Dependent Claim
17 words
The lipase substrate of claim 1, wherein R and/or R 1 contain 8 to 12 carbon atoms.
Claim 4
Dependent Claim
14 words
The lipase substrate of claim 1, wherein A contains 3 to 7 carbon atoms.
Claim 5
Dependent Claim
4 words
1,2-O-Dioctyl-rac-glycero-3-azelaic acid resorufin ester.
Claim 6
Dependent Claim
4 words
1,2-O-Dioctyl-rac-glycero-3-azelaic acid resorufin ester.
Claim 7
Dependent Claim
4 words
1,2-O-Dioctyl-rac-glycero-3-pimelic acid resorufin ester.
Claim 8
Dependent Claim
4 words
1,2-O-Dioctyl-rac-glycero-3-pimelic acid resorufin ester.
Claim 9
Dependent Claim
4 words
1,2-O-Dioctyl-rac-glycero-3-glutaric acid resorufin ester.
Claim 10
Dependent Claim
4 words
1,2-O-Dioctyl-rac-glycero-3-glutaric acid resorufin ester.
Claim 11
Dependent Claim
4 words
1,2-O-Dioctyl-rac-glycero-3-glutaric acid resorufin ester.
Claim 12
Dependent Claim
4 words
1,2-O-Dioctyl-rac-glycero-3-glutaric acid resorufin ester.
References Cited (Backward Citations)
DE3342106A1
Cited
US4667032A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-07-10 | 1993-01-09 | 1993-07-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-07-10 | 1997-01-09 | 1997-07-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-07-10 | 2001-01-09 | 2001-07-11 | $7700.00 | Window Closed / Lapsed |