US4843012A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Novel composition for nucleic acid purification
Assignee: Genetics Institute LLC
•
Filing: 1986-09-17
•
Granted: 1989-06-27
•
Expires: 2006-09-17
Abstract
Abstract A storage stable single phase aqueous composition is provided which is useful in isolatin nucleic acids from cell or virus cultures.
Inventors
- 👤 David A. DeBonville
- 👤 Gerard E. Riedel
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 22:37
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/10; C12N15/1003; C12N1/00; C12N1/08; Y; Y10; Y10S; Y10S435/00; Y10S435/803
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
103 words
A single phase aqueous composition that is stable against a separation of phases for at least thirty days of storage at about 25° C. and is useful in isolating nucleic acids from cell or virus cultures comprising: (a) about 1.6 to 3.2M potassium acetate; (b) about 5 to 15% by weight phenol; (c) about 5 to 15% by weight chloroform; and (d) glacial acetic acid in an amount such that the weight ratio of potassium acetate to glacial acetic acid is 3:2, wherein the weight ratio of (a) to the combination of (b) and (c) is in the range of 4:1 to 4:3.
Claim 2
Dependent Claim
103 words
A single phase aqueous composition that is stable against a separation of phases for at least thirty days of storage at about 25° C. and is useful in isolating nucleic acids from cell or virus cultures comprising: (a) about 1.6 to 3.2M potassium acetate; (b) about 5 to 15% by weight phenol; (c) about 5 to 15% by weight chloroform; and (d) glacial acetic acid in an amount such that the weight ratio of potassium acetate to glacial acetic acid is 3:2, wherein the weight ratio of (a) to the combination of (b) and (c) is in the range of 4:1 to 4:3.
Claim 3
Dependent Claim
26 words
The composition according to claim 1 further comprising: (e) 0 to about 1.2% by weight isoamyl alcohol; and (f) 0 to about 0.12% by weight 8-hydroxyquinoline.
Claim 4
Dependent Claim
24 words
A sealed package useful in an automated procedure for isolating DNA from cell cultures containing the stable single phase aqueous composition of claim 1.
Claim 5
Dependent Claim
31 words
In a nucleic acid isolation procedure wherein cells in a cell culture are lysed and nucleic acids are extracted from proteinaceous contaminants, the improvement comprising employing the composition of claim 1.
Claim 6
Dependent Claim
71 words
A single phase aqueous composition that is stable against a separation of phases for at least thirty days of storage at about 25° C. and is useful in isolating nucleic acids from cell or virus cultures comprising: (a) 2.4M potassium acetate; (b) 10M acetic acid; (c) 5 to 15% by weight phenol; (d) 5 to 15% by weight chloroform; (e) 0.2% by weight isoamyl alcohol; and (f) 0.02% by weight hydroxyquinoline.
Claim 7
Dependent Claim
71 words
A single phase aqueous composition that is stable against a separation of phases for at least thirty days of storage at about 25° C. and is useful in isolating nucleic acids from cell or virus cultures comprising: (a) 2.4M potassium acetate; (b) 10M acetic acid; (c) 5 to 15% by weight phenol; (d) 5 to 15% by weight chloroform; (e) 0.2% by weight isoamyl alcohol; and (f) 0.02% by weight hydroxyquinoline.
References Cited (Backward Citations)
SU861399A1
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-06-26 | 1992-12-26 | 1993-06-27 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-06-26 | 1996-12-26 | 1997-06-27 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-06-26 | 2000-12-26 | 2001-06-27 | $7700.00 | Window Closed / Lapsed |