US4839281A
Expired / Lapsed
US
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Lactobacillus strains and methods of selection
Assignee: GORBACH-GOLDIN Corp
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Filing: 1985-04-17
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Granted: 1989-06-13
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Expires: 2005-04-17
Abstract
Abstract A biologically pure culture of a strain of bacteria of a Lactobacillus species in which the bacteria have avid adherence to intestinal cells, are able to survive at low pH, and produce large amounts of lactic acid.
Inventors
- 👤 Sherwood L. Gorbach
- 👤 Barry R. Goldin
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 03:01
CPC Classifications
A; A61; A61K; A61K35/00; A61K35/66; A61K35/74; A61K35/741; A61K35/744; A61K35/747; C; C12; C12N; C12N1/00; C12N1/20; C12N1/205; A23; A23L; A23L33/00; A23L33/10; A23L33/135; A61P; A61P1/00; A61P1/04; A61P1/12; A61P1/14; C12R; C12R2001/00; C12R2001/01; C12R2001/225; C12R2001/23; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/853; Y10S435/854
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
50 words
A biologically pure culture of bacteria of a Lactobacillus species, said bacteria being characterized in that they have the same ability to attach mucosal cells of the human intestinal tract as that exhibited by the Lactobacillus bacteria deposited in the American Type Culture Collection and given ATCC Accession No. 53103.
Claim 2
Dependent Claim
50 words
A biologically pure culture of bacteria of a Lactobacillus species, said bacteria being characterized in that they have the same ability to attach mucosal cells of the human intestinal tract as that exhibited by the Lactobacillus bacteria deposited in the American Type Culture Collection and given ATCC Accession No. 53103.
Claim 3
Dependent Claim
10 words
The culture of claim 1, having ATCC Accession No. 53103.
Claim 4
Independent Claim
91 words
A method of isolating a therapeutically useful strain of a Lactobacillus species, said method comprising carrying out the following screening steps on a sample containing a multiplicity of bacterial strains: (a) isolating from said sample bacteria of a Lactobacillus species, (b) coincubating bacteria of said sample with gastrointestinal cells, and (c) selecting bacteria capable of adhering to said cells at a rate of an average of at least 50 bacterial cells per gastrointestinal cell in five minutes of coincubation. Lactobacillus species bacteria capable of so adhering comprising said therapeutically useful strain.
Claim 5
Independent Claim
91 words
A method of isolating a therapeutically useful strain of a Lactobacillus species, said method comprising carrying out the following screening steps on a sample containing a multiplicity of bacterial strains: (a) isolating from said sample bacteria of a Lactobacillus species, (b) coincubating bacteria of said sample with gastrointestinal cells, and (c) selecting bacteria capable of adhering to said cells at a rate of an average of at least 50 bacterial cells per gastrointestinal cell in five minutes of coincubation. Lactobacillus species bacteria capable of so adhering comprising said therapeutically useful strain.
Claim 6
Dependent Claim
31 words
The method of claim 3, further comprising the step of incubating bacterial of said sample in nutrient medium at a pH below 5.5 and selecting bacteria which proliferate in said medium.
Claim 7
Dependent Claim
31 words
The method of claim 3 further comprising the step of incubating bacteria of said sample in nutrient medium containing at least 0.1% bile and selecting bacteria which proliferate in said medium.
Claim 8
Dependent Claim
21 words
A biologically pure culture of bacteria of a Lactobacillus species, wherein said culture is selected by the process of claim 3.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-06-12 | 1992-12-12 | 1993-06-13 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-06-12 | 1996-12-12 | 1997-06-13 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-06-12 | 2000-12-12 | 2001-06-13 | $7700.00 | Window Closed / Lapsed |