Process for purifying a protein

Claim 2

A process for the purification of a human gamma interferon protein bearing less than three surface cysteine residues, which forms highly insoluble aggregates during the growth of cells transformed with DNA sequences coding for the protein, comprising the steps of: (a) breaking said transformed cells, (b) extracting said protein by solubilization of the protein with a chaotrope (C) renaturing said protein by rapid dilution or removal of the chaotrope, and (d) purifying said protein by covalent chromatography on a thiol resin followed by molecular sizing chromatography to obtain said protein in its native conformation.

Claim 3

A process for the purification of IFN-γ from inclusion bodies formed during the growth of cells transformed with DNA sequences coding for IFN-γ, comprising the steps; (a) breaking the cells, (b) extracting the IFN-γ by solubilization with a chaotrope, (c) adjusting the chaotrope containing solubilized IFN-γ solution formed in step (b) to an IFN-γ concentration of approximately 1 mg/ml with a chaotrope-containing buffer, (d) renaturing the IFN-γ by dilution of the chaotrope extract with aqueous phsyiological buffer containing phosphate buffered saline and carbohydrate, optionally also containing a detergent having affinity for solubilized lipid in said extract, (e) purifying the IFN-γ by (i) covalent chromatography on a thiol resin capable of forming disulfide linkages with the surface cysteines of said IFN-γ, and thereafter (ii) molecular sizing chromatography, and (f) recovering IFN-γ in its native conformation.

Claim 4

A process for the purification of IFN-γ from inclusion bodies formed during the growth of cells transformed with DNA sequences coding for IFN-γ, comprising the steps; (a) breaking the cells, (b) extracting the IFN-γ by solubilization with a chaotrope, (c) adjusting the chaotrope containing solubilized IFN-γ solution formed in step (b) to an IFN-γ concentration of approximately 1 mg/ml with a chaotrope-containing buffer, (d) renaturing the IFN-γ by dilution of the chaotrope extract with aqueous phsyiological buffer containing phosphate buffered saline and carbohydrate, optionally also containing a detergent having affinity for solubilized lipid in said extract, (e) purifying the IFN-γ by (i) covalent chromatography on a thiol resin capable of forming disulfide linkages with the surface cysteines of said IFN-γ, and thereafter (ii) molecular sizing chromatography, and (f) recovering IFN-γ in its native conformation.

Claim 5

The process according to claim 2, wherein the chaotrope is urea or guanidine hydrochloride.

Claim 6

The process according to claim 2, wherein said carbohydrate is sucrose.

Claim 7

The process according to claim 2, wherein the IFN-γ is eluted from the thiol resin with a reductant.

Claim 8

The process according to claim 5, wherein said reductant is dithiothreitol or cysteine.