US4828987A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Avian retrovirus-bovine growth hormone DNA
Assignee: Merck and Co Inc
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Filing: 1984-05-14
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Granted: 1989-05-09
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Expires: 2004-05-14
Abstract
Abstract Recombinant DNA molecules in which an avian retroviral long terminal repeat (LTR) has been ligated to the bovine growth hormone gene are described herein. The retroviral LTR was derived from a plasmid clone of a Schmidt Rupin strain of Rous Sarcoma Virus while the bovine growth hormone (BGH) gene was derived from a lambda-bacteriophage genomic library. Using a transient eucaryotic expression assay system, the plasmid constructs were screened for their ability to direct expression and secretion of bovine growth hormone. These constructs were co-transformed into a mammalian cell (mouse) culture in order to obtain a stable cell culture secreting large amounts of bovine growth hormone.
Inventors
- 👤 John J. Kopchick
- 👤 Frederick C. Leung
- 👤 Thomas J. Livelli
- 👤 Richard H. Malavarca
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 14:17
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/85; C12N15/86; C07; C07K; C07K14/00; C07K14/435; C07K14/575; C07K14/61; C12N15/87; C12N15/90; C12N2740/00; C12N2740/00011; C12N2740/10011; C12N2740/11011; C12N2740/11041; C12N2740/11043
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
2 words
Plasmid pBGH-4.
Claim 2
Dependent Claim
2 words
Plasmid pBGH-4.
Claim 3
Dependent Claim
8 words
A mouse fibroblast cell line containing plasid pBGH-4.
Claim 4
Dependent Claim
8 words
A mouse fibroblast cell line containing plasid pBGH-4.
Claim 5
Dependent Claim
6 words
The mouse fibroblast cell line BGH-4-3.
Claim 6
Dependent Claim
6 words
The mouse fibroblast cell line BGH-4-3.
Claim 7
Dependent Claim
6 words
The mouse fibroblast cell line BGH-4-13.
Claim 8
Dependent Claim
6 words
The mouse fibroblast cell line BGH-4-13.
Claim 9
Independent Claim
30 words
A method of producing bovine growth hormone comprising introducing plasmid pBGH-4 into rat GH3 cells by DEAE-Dextran mediated transfection, and incubating the cells at 37° C. for about 120 hours.
Claim 10
Independent Claim
30 words
A method of producing bovine growth hormone comprising introducing plasmid pBGH-4 into rat GH3 cells by DEAE-Dextran mediated transfection, and incubating the cells at 37° C. for about 120 hours.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-05-08 | 1992-11-07 | 1993-05-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-05-08 | 1996-11-07 | 1997-05-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-05-08 | 2000-11-07 | 2001-05-09 | $7700.00 | Window Closed / Lapsed |