Therapeutic drug monitoring and analyte determination utilizing micellar chromatography

Claim 3

A method as defined in claim 1, wherein said bodily fluid is serum.

Claim 4

A method as defined in claim 1, wherein said bodily fluid is urine.

Claim 5

A method as defined in claim 1, wherein said surfactant is sodium dodecyl sulfate.

Claim 6

A method as defined in claim 1, wherein said surfactant is polyoxyethylene dodecyl.

Claim 7

A method as defined in claim 1, where in step (a) the anionic or nonionic surfactant is at a concentration of about 1.01 times the critical micelle concentration to about 0.99 times the Krafft point.

Claim 8

A method as defined in claim 1, where in step (a) the bodily fluid is injected at a flow rate effective to provide in step (b) a flow rate from said column of about 10 μl/min. to about 20 ml/min.

Claim 9

A method as defined in claim 1, wherein propanol is added to the mobile phase in a range of above 0% to about 40% of the volume of the mobile phase.

Claim 10

A method as defined in claim 1, wherein the pH of the mobile phase is adjusted to a range of aobut 2 to about 7.

Claim 11

A method as defined in claim 1, wherein said bodily fluid is saliva, peritoneal fluid, cerebrospinal fluid, blood plasma, sweat, semen, feces, mucous-containing fluid, tears, vaginal whole blood, embryonic fluid.

Claim 12

A kit useful for a determination of a drug concentration in a bodily fluid which consists essentially of: a carrier having vertical sides, a bottom affixed to said sides and a multiplicity of compartments each including surfactant as a component for preparation of a mobile phase for chromatographic analysis; a second container means including a solvent for adding to the surfactant for preparation of a mobile phase; a third container means including known drug standards in an appropriate body fluid at predetermined values for standardization of a chromatographic column; a fourth container means including an appropriate body fluid containing no drugs for calibration of the chromatographic column; a fifth container means including appropriate organic modifiers for preparation of a mobile phase; a sixth container including appropriate inorganic modifiers for preparation of a mobile phase; a seventh container means including pH adjustment materials; and a packet with instructions for preparation of the mobile phase and calibration of the chromatographic column for determination of the concentration of the drug.

Claim 13

A kit useful for a determination of a drug concentration in a bodily fluid which consists essentially of: a carrier having vertical sides, a bottom affixed to said sides and a multiplicity of compartments each including surfactant as a component for preparation of a mobile phase for chromatographic analysis; a second container means including a solvent for adding to the surfactant for preparation of a mobile phase; a third container means including known drug standards in an appropriate body fluid at predetermined values for standardization of a chromatographic column; a fourth container means including an appropriate body fluid containing no drugs for calibration of the chromatographic column; a fifth container means including appropriate organic modifiers for preparation of a mobile phase; a sixth container including appropriate inorganic modifiers for preparation of a mobile phase; a seventh container means including pH adjustment materials; and a packet with instructions for preparation of the mobile phase and calibration of the chromatographic column for determination of the concentration of the drug.

Claim 14

A kit of claim 11, containing an eighth container means including a chromatographic column.