US4828695A
Expired / Lapsed
US
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Packaging material for high pressure liquid chromatography and method of making the same
Assignee: Yamamura Chemical Laboratories Co Ltd
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Filing: 1988-02-10
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Granted: 1989-05-09
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Expires: 2008-02-10
Abstract
Abstract A novel packing material suitable for high pressure liquid chromatography comprising the chemically bonded, crosslinked reaction product of porous inorganic particles, with an organic silane compound having epoxy groups on the surface of the particles, and then with polyvinyl alcohol, in which the hydroxyl groups of the polyvinyl alcohol and the epoxy groups on the silane moieties react to form ether bonds, which cover almost or almost completely the residual silica hydroxyl groups on the surface of the support.
Inventors
- 👤 Ryuji Yamamura
- 👤 Mikio Tsuboi
- 👤 Keishi Kitagawa
- 👤 Masaru Abe
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 04:57
CPC Classifications
B; B01; B01D; B01D15/00; B01D15/08; B01J; B01J20/00; B01J20/28; B01J20/28002; B01J20/28004; B01J20/28054; B01J20/28057; B01J20/28078; B01J20/281; B01J20/286; B01J20/30; B01J20/3092; B01J20/32; B01J20/3202; B01J20/3204; B01J20/3231; B01J20/3242; B01J20/3244; B01J20/3246; B01J20/3257; B01J20/3263; B01J20/3268; B01J20/327; B01J2220/00; B01J2220/50; B01J2220/54; B01J2220/58; Y; Y10; Y10T; Y10T428/00; Y10T428/29; Y10T428/2982; Y10T428/2991; Y10T428/2993; Y10T428/2996; Y10T428/2998
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
127 words
A packing material for analysis and/or separation of hydrophilic compounds comprising the chemically bound, crosslinked reaction product of porous inorganic particles having an average particle diameter of about 1 to about 1000 microns, a surface area of about 1 to about 800 square meters per gram and an average pore size of about 10 to about 4000 Angstrom units, with an organic silane compound having epoxy groups on the surface of the particles, and then with polyvinyl alcohol having an average molecular weight of about 100 to about 200000, in which the hydroxyl groups of the polyvinyl alcohol and the epoxy groups on the silane moieties react to form ether bonds, whereby the residual silica hydroxyl groups on the surface of the support are almost completely covered.
Claim 2
Dependent Claim
127 words
A packing material for analysis and/or separation of hydrophilic compounds comprising the chemically bound, crosslinked reaction product of porous inorganic particles having an average particle diameter of about 1 to about 1000 microns, a surface area of about 1 to about 800 square meters per gram and an average pore size of about 10 to about 4000 Angstrom units, with an organic silane compound having epoxy groups on the surface of the particles, and then with polyvinyl alcohol having an average molecular weight of about 100 to about 200000, in which the hydroxyl groups of the polyvinyl alcohol and the epoxy groups on the silane moieties react to form ether bonds, whereby the residual silica hydroxyl groups on the surface of the support are almost completely covered.
Claim 3
Dependent Claim
53 words
A packing material as set forth in claim 1, in which the porous inorganic particles have an average particle diameter of about 2 to about 300 microns, a surface area of about 50 to about 500 square meters per gram and an average pore size of about 20 to about 2000 Angstrom units.
Claim 4
Dependent Claim
20 words
A packing material as set forth in claim 1 or 2, in which the porous particles are microparticulate silica gel.
Claim 5
Dependent Claim
86 words
A packing material as set forth in claim 1, in which the organic silane compound is the following general formula: ##STR2## in which R 1 is epoxy group, R 2 is a member selected from the group consisting of epoxy, methoxy, ethoxy, methyl and ethyl groups and halogen atom, X is a member selected from the group consisting of methoxy, ethoxy, methyl and ethyl groups and halogen atom, and Y is a member selected from the group consisting of methoxy and ethoxy groups and halogen atom.
Claim 6
Dependent Claim
18 words
A packing material as set forth in claim 4, in which the organic silane compound is γ-glycidoxypropyl trimethoxysilane.
Claim 7
Dependent Claim
33 words
A packing material as set forth in claim 1, in which the polyvinyl alcohol has an average molecular weight about 100 to about 200000 of which uniform and non-uniform degrees of polymerication coexist.
Claim 8
Dependent Claim
51 words
A packing material as set forth in claim 6, in which polyvinyl alcohol is mixed with a member selected from the group consisting of poly o-hydroxystyrene and methylol derivatives thereof, polyoxymethylene, polyethylene glycol having a polymerization degree of 10 or more, and polysaccharide composed of four or more moleculars of monosaccharide.
Claim 9
Independent Claim
141 words
A method of making a packing material for analysis and/or separation of hydrophilic compounds, which comprises reacting porous inorganic particles having an average particle diameter of about 1 to about 1000 microns, a surface area of about 1 to about 800 square meters per gram and an average pore size of about 10 to about 4000 Angstrom units, with an organic silane compound having epoxy group, whereby the silane compound is bound chemically to the surface of the particles without ring-opening of the epoxy groups, and then reacting the resultant product with polyvinyl alcohol having an average molecular weight of about 100 to about 200000, whereby the hydroxyl groups of the polyvinyl alcohol and the epoxy groups on the silane moieties react to form ether bonds, which cover almost completely the residual silica hydroxyl groups on the surface of the support.
Claim 10
Independent Claim
141 words
A method of making a packing material for analysis and/or separation of hydrophilic compounds, which comprises reacting porous inorganic particles having an average particle diameter of about 1 to about 1000 microns, a surface area of about 1 to about 800 square meters per gram and an average pore size of about 10 to about 4000 Angstrom units, with an organic silane compound having epoxy group, whereby the silane compound is bound chemically to the surface of the particles without ring-opening of the epoxy groups, and then reacting the resultant product with polyvinyl alcohol having an average molecular weight of about 100 to about 200000, whereby the hydroxyl groups of the polyvinyl alcohol and the epoxy groups on the silane moieties react to form ether bonds, which cover almost completely the residual silica hydroxyl groups on the surface of the support.
Claim 11
Dependent Claim
18 words
A chromatographic column suitable for high pressure liquid chromatography packed with the material set forth in claim 1.
References Cited (Backward Citations)
US4314032A
Cited
US4724207A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-05-08 | 1992-11-07 | 1993-05-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-05-08 | 1996-11-07 | 1997-05-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-05-08 | 2000-11-07 | 2001-05-09 | $7700.00 | Window Closed / Lapsed |