US4822741A
Expired / Lapsed
US
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Biocompatible polyorganosiloxane composition for cell culture apparatus
Assignee: Flexcell International Corp
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Filing: 1988-04-11
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Granted: 1989-04-18
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Expires: 2008-04-11
Abstract
Abstract A polyorganosiloxane composition having a biocompatible surface thereon is disclosed. The biocompatible surface results from the derivatization, or amination, of the surface intended for cell contact. More specifically, the present invention is a polyorganosiloxane composition in which the surface is either treated with a primary amine and optional peptide or the surface is co-cured with a primary amine-containing silane or siloxane. The aminated polyorganosiloxane has utility as a cell culture substrate or in a variety of artificial organ applicatons such as breast implants, synthetic blood vessels, joints, tendons and heart valves. A vacuum apparatus for use with specialized cell cultrue plates incorporating the biocompatible polyorganosiloxane composition is also disclosed.
Inventors
- 👤 Albert J. Banes
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 21:16
CPC Classifications
C; C12; C12M; C12M25/00; A; A61; A61L; A61L27/00; A61L27/14; A61L27/18; C08; C08J; C08J7/00; C08J7/12; C08J7/14; C12M23/00; C12M23/02; C12M23/12; C12M35/00; C12M35/04; C12N; C12N5/00; C12N5/0068; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54353; G01N33/544; G01N33/545; C08J2383/00; C08J2383/04; C12N2533/00; C12N2533/30; G01N2203/00; G01N2203/0014; G01N2203/0023; G01N2203/003; G01N2203/0042; G01N2203/0044; G01N2203/0046; G01N2203/0058; G01N2203/0089; G01N35/00; G01N35/02; G01N35/028; Y; Y10; Y10T; Y10T428/00; Y10T428/24; Y10T428/24149; Y10T428/24628; Y10T428/24661; Y10T428/31504; Y10T428/31652; Y10T428/31663
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
74 words
A method for treating the surface of a polyorganosiloxane composition comprising the steps of: (a) contacting the polyorganosiloxane surface with an acid selected from the group consisting of hydrochloric acid, hydrofluoric acid and hydrobromic acid and then decanting said acid; (b) contacting the polyorganosiloxane surface with an amine selected from the group consisting of ammonium hydroxide, ammonium chloride and ammonium bicarbonate and then decanting said amine; and (c) washing said polyorganosiloxane surface with water.
Claim 2
Independent Claim
74 words
A method for treating the surface of a polyorganosiloxane composition comprising the steps of: (a) contacting the polyorganosiloxane surface with an acid selected from the group consisting of hydrochloric acid, hydrofluoric acid and hydrobromic acid and then decanting said acid; (b) contacting the polyorganosiloxane surface with an amine selected from the group consisting of ammonium hydroxide, ammonium chloride and ammonium bicarbonate and then decanting said amine; and (c) washing said polyorganosiloxane surface with water.
Claim 3
Dependent Claim
37 words
The method according to claim 1, wherein step (c) further comprises the step of washing said polyorganosiloxane surface with water, sequentially contacting said surface with an aldehyde and an aqueous peptide, and washing said surface with water.
Claim 4
Dependent Claim
45 words
The method according to claim 1, wherein step (c) further comprises the step of washing said polyorganosiloxane surface with water, sequentially contacting said surface with glutaraldehyde and an aqueous peptide wherein said peptide has both amine and carboxyl functionality, and washing said surface with water.
Claim 5
Dependent Claim
31 words
The method according to claim 1, wherein step (a) further comprises the step of contacting the polyorganosiloxane surface with 0.5 to 1.0 ml. per cm 2 of 1 N. hydrochloric acide.
Claim 6
Dependent Claim
31 words
The method according to claim 1, wherein step (b) further comprises the step of contacting the polyorganosiloxane surface with 0.5 to 1 ml per cm 2 of 1 M. ammonium hydroxide;.
Claim 7
Dependent Claim
31 words
The method according to claim 1, further comprising the step of (d) incorporating said polyorganosiloxane composition onto a solid means for supporting a cell culture to form a cell culture substrate.
Claim 8
Dependent Claim
11 words
The product prepared in accordance with any one of claims 1-6.
Claim 9
Independent Claim
41 words
A method for treating the surface of a polyorganosiloxane composition, comprising the co-curing of a polyorganosiloxane composition with an adjacent layer of a compound selected from the group consisting of primary amine-containing silanes, carboxyl-containing silanes, primary amine-containing siloxanes and carboxyl-containing siloxanes.
Claim 10
Independent Claim
41 words
A method for treating the surface of a polyorganosiloxane composition, comprising the co-curing of a polyorganosiloxane composition with an adjacent layer of a compound selected from the group consisting of primary amine-containing silanes, carboxyl-containing silanes, primary amine-containing siloxanes and carboxyl-containing siloxanes.
Claim 11
Dependent Claim
52 words
The method according to claim 8 wherein said polyorganosiloxane composition is co-cured with a compound selected from the group consisting of 3-aminopropyltriethoxysilane, 2-aminoethyitrimethoxysilane, trimethylsilylformic acid, 3-(trichlorosilyl) butanoic acid, and 1,1,1-trichioro-N-(trimethylsilyl) silanamine suspended in an aqueous or aqueous buffer carrier, by maintaining the adjacent compositions at about room temperature for about twenty-four hours.
Claim 12
Dependent Claim
24 words
The method according to claim 8 wherein co-curing is effected at about 60° C. and ambient pressure over a period of about 15 minutes.
Claim 13
Dependent Claim
11 words
The produce prepared in accordance with any one of claims 8-10.
Claim 14
Dependent Claim
92 words
A cell culture substrate comprising: solid means for supporting a cell culture having at least one surface further comprising a polyorganosiloxane composition wherein a surface of said polyorganosiloxane composition is treated by: (a) contacting the polyorganosiloxane surface with an acid selected from the group consisting of hydrochloric acid, hydrofluoric acid, and hydrobromic acid, and then decanting said acid; (b) contacting the polyorganosiloxane surface with an amine selected from the group consisting of ammonium hydroxide, ammonium chloride and ammonium bicarbonate and then decanting said amine; and (c) washing said polyorganosiloxane surface with water.
Claim 15
Dependent Claim
92 words
A cell culture substrate comprising: solid means for supporting a cell culture having at least one surface further comprising a polyorganosiloxane composition wherein a surface of said polyorganosiloxane composition is treated by: (a) contacting the polyorganosiloxane surface with an acid selected from the group consisting of hydrochloric acid, hydrofluoric acid, and hydrobromic acid, and then decanting said acid; (b) contacting the polyorganosiloxane surface with an amine selected from the group consisting of ammonium hydroxide, ammonium chloride and ammonium bicarbonate and then decanting said amine; and (c) washing said polyorganosiloxane surface with water.
Claim 16
Dependent Claim
39 words
The cell culture substrate according to claim 12 wherein step (c) further comprises the step of washing said polyorganosiloxane surface with water, sequentially contacting said surface with an aldehyde and an aqueous peptide, and washing said surface with water.
Claim 17
Dependent Claim
47 words
The cell culture substrate according to claim 12, wwherein step (c) further comprises the step of washing said polyorganosiloxane surface with water, sequentially contacting said surface with glutaraldehyde and an aqueous peptide wherein said peptide has both amine and carboxyl functionality, and washing said surface with water.
Claim 18
Dependent Claim
33 words
The cell culture substrate according to claim 12 wherein step (a) further comprises the step of contacting the polyorganosiloxane surface with 0.5 to 1 ml. per cm 2 of 1 N. hydrochloric acid.
Claim 19
Dependent Claim
33 words
The cell culture substrate according to claim 12 whrein step (b) further comprises the step of contacting the polyorganosiloxane surface with 0.5 to 1 ml. per cm 2 of 1 M. ammonium hydroxide.
Claim 20
Dependent Claim
32 words
The cell culture substrate according to claim 12 further comprising the step of incorporating said polyorganosiloxane composition onto a solid means for supporting a cell culture to form a cell culture substrate.
Claim 21
Dependent Claim
17 words
The cell culture substrate according to claim 12 wherein said solid means is a multi-well polystyrene plate.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-04-17 | 1992-10-17 | 1993-04-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-04-17 | 1996-10-17 | 1997-04-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-04-17 | 2000-10-17 | 2001-04-18 | $7700.00 | Window Closed / Lapsed |