US4822490A
Expired / Lapsed
US
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Bacterial compositon and method for recovery of oil-polluted water and soil
Assignee: Zapakno-Sibirsky Nauchno Issle Geologorazvedochny Neftyanoi Inst
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Filing: 1986-05-29
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Granted: 1989-04-18
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Expires: 2006-05-29
Abstract
Abstract PCT No. PCT/SU86/00053 Sec. 371 Date Jan. 15, 1988 Sec. 102(e) Date Jan. 15, 1988 PCT Filed May 29, 1986 PCT Pub. No. WO87/07316 PCT Pub. Date Dec. 3, 1987.The composition intended for recovery of oil-polluted water and soil contains Pseudomonas putida-36 as an active biocomponent deposited under No. B- 2443 and a salt mixture as a mineral component, mass %: 34.26-37.12 of KNO3; 28.66-31.28 of NH4Cl; 25.42-28.71 of NH4H2PO4; and 2.89-11.66 of NH4NO3. The mass ratio between the biocomponent and mineral component is 1:26-32. The composition can be utilized with or without the carrier in the form of a solid inert substance or water.
Inventors
- 👤 Vladimir N. Dyadechko
- 👤 Ivan I. Nesterov
- 👤 Lidia E. Tolstokorova
- 👤 Svetlana V. Platonova
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 23:46
CPC Classifications
C; C02; C02F; C02F1/00; C02F1/68; C02F1/681; B; B09; B09C; B09C1/00; B09C1/10; C02F3/00; C02F3/02; C02F3/10; C02F3/34; C09; C09K; C09K3/00; C09K3/32; Y; Y02; Y02W; Y02W10/00; Y02W10/10; Y02W10/30; Y02W10/37; Y10; Y10S; Y10S210/00; Y10S210/918; Y10S210/922; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/874; Y10S435/877
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
81 words
A bacterial composition for cleaning water and soil from oil pollution comprising the bacteria Pseudomonas putida-36 as an active biocomponent, deposited under No. B-2443, and a mineral component comprising a mixture of mineral salts, having a percent by weight of the mixture of from 34.26-37.12% KNO 3 ; 28.66-31.28% NH 4 Cl; 26.42-28.71% NH 4 H 2 PO 4 and 11.66-2.89% NH 4 NO 3 , wherein the ratio of the biocomponent to the mineral component being from 1.26 to 1:32.
Claim 2
Dependent Claim
81 words
A bacterial composition for cleaning water and soil from oil pollution comprising the bacteria Pseudomonas putida-36 as an active biocomponent, deposited under No. B-2443, and a mineral component comprising a mixture of mineral salts, having a percent by weight of the mixture of from 34.26-37.12% KNO 3 ; 28.66-31.28% NH 4 Cl; 26.42-28.71% NH 4 H 2 PO 4 and 11.66-2.89% NH 4 NO 3 , wherein the ratio of the biocomponent to the mineral component being from 1.26 to 1:32.
Claim 3
Dependent Claim
26 words
A composition of claim 1, wherein the content of living cells in said biocomponent is from 1.8×10 10 to 3.6×10 12 per gram of dry solid.
Claim 4
Dependent Claim
9 words
A composition of claim 1 containing an inert filler.
Claim 5
Dependent Claim
45 words
A composition of claim 3, wherein the inert filler is a solid composition selected from the group consisting of talc, paraffin, kaolin and diatomaceous earth, and is present in a ratio of filler and mixture of biocomponent and mineral component is from 30:1 to 50:1.
Claim 6
Dependent Claim
11 words
A composition of claim 3, wherein the inert filler is water.
Claim 7
Dependent Claim
29 words
A composition of claim 5 comprising from 0.07% to 0.08% by weight of the mineral salts and 10 4 to 10 6 living cells of the biocomponent per milliliter.
Claim 8
Independent Claim
84 words
A method of cleaning water and soil from oil pollution, which comprises contacting the water or soil with a composition comprising a bacteria Pseudomonas putida-36 deposited under No. B-2443 as an active biocomponent and a mineral component comprising a mixture of salts in a percent by weight of 34.26-37.12% KNO 3 ; 28.66-31.28% NH 4 Cl; 26.42-28.71% NH 4 H 2 PO 4 and 11.66-2.89% NH 4 NO 3 , the weight ratio of the biocomponent to the mineral component being 1:26 to 1:32.
Claim 9
Independent Claim
84 words
A method of cleaning water and soil from oil pollution, which comprises contacting the water or soil with a composition comprising a bacteria Pseudomonas putida-36 deposited under No. B-2443 as an active biocomponent and a mineral component comprising a mixture of salts in a percent by weight of 34.26-37.12% KNO 3 ; 28.66-31.28% NH 4 Cl; 26.42-28.71% NH 4 H 2 PO 4 and 11.66-2.89% NH 4 NO 3 , the weight ratio of the biocomponent to the mineral component being 1:26 to 1:32.
Claim 10
Dependent Claim
27 words
A method of claim 7, wherein the number of living cells in the bicomponent is from 1.8×10 10 to 3.6×10 12 per gram of a dry biomass.
Claim 11
Dependent Claim
15 words
A method of claim 7, wherein the composition is in admixture with an inert filler.
Claim 12
Dependent Claim
40 words
A method of claim 9, wherein the inert filler is a solid selected from the group consisting of talc, paraffin, diotomaceous earth and kaolin in a weight ratio of the inert filler to the composition of from 30:1 to 50:1.
Claim 13
Dependent Claim
25 words
A method of claim 8, wherein from 350 to 800 milligrams of the composition is applied to a square meter of surface to be treated.
Claim 14
Dependent Claim
39 words
A method of claim 9, wherein the composition is in admixture with water to provide a mixture containing from 0.07 to 0.08% by weight mineral component and from 10 4 to 10 6 living cells per milliliter of admixture.
Claim 15
Dependent Claim
25 words
A method of claim 7, wherein the admixture is applied in an amount of 0.5 to 1 liter per square meter of a treated surface.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1992-04-17 | 1992-10-17 | 1993-04-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-04-17 | 1996-10-17 | 1997-04-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-04-17 | 2000-10-17 | 2001-04-18 | $7700.00 | Window Closed / Lapsed |