US4804849A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method and apparatus for determining concentrations of chlorophyll and the rate of primary production in water
Assignee: BIOSPHERICAL INSTRUMENTS IN A CORP OF CA
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Filing: 1987-01-16
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Granted: 1989-02-14
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Expires: 2007-01-16
Abstract
Abstract Apparatus is provided for optically measuring scalar irradiance or incident flux of radiant energy and for optically measuring naturally occurring chlorophyll fluorescence or upwelling radiance in a parcel of water in a natural setting. From a comparison of the two measurements, rate of primary photosynthetic production is calculated by appropriately programmed computer means. Readout means are provided to indicate the rate of primary production. Further, concentrations of chlorophyll that generate the primary production are also determined by means of the apparatus of this invention. The method of this invention, employing measurement instruments of the type disclosed, enables the determination of concentrations of chlorophyll and primary production in parcels of water.
Inventors
- 👤 Charles R. Booth
- 👤 Dale A. Kiefer
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 22:03
CPC Classifications
G; G01; G01N; G01N21/00; G01N21/62; G01N21/63; G01N21/64; G01N21/6486; G01N21/84; G01N21/85; G01N21/8507; G01N33/00; G01N33/18; G01N33/186; G01N33/1866; G01J; G01J1/00; G01J1/02; G01J1/04; G01J1/0488; G01J1/06; G01J1/42; G01J1/4228; G01N21/17; G01N2021/178; G01N2021/1782; G01N2021/635; G01N2201/00; G01N2201/02; G01N2201/021; G01N2201/0212; G01N2201/0218; G01N2201/06; G01N2201/061; G01N2201/0616
Claims Analysis
Total Claims: 66
Claim 1
Independent Claim
150 words
A method for determining the natural rate of photosynthetic primary production driven by natural light in a predetermined parcel of water, said method comprising the steps of: sensing excitation energy resulting from naturally occurring radiant flux density applied by natural light to the predetermined parcel of water and existing in the parcel of water over a period of time; producing an electrical signal representative of the sensed naturally occurring excitation energy; sensing the naturally occurring fluorescent emission from within the predetermined parcel of water which emission exists in the parcel of water over a period of time as a result of the existing naturally occurring applied excitation energy; producing an electrical signal representative of the sensed fluorescent emission; combining the electrical signals representative of sensed naturally occurring excitation energy and sensed fluorescent emission; and calculating the rate of primary production in the predetermined parcel of water from said electrical signals.
Claim 2
Independent Claim
150 words
A method for determining the natural rate of photosynthetic primary production driven by natural light in a predetermined parcel of water, said method comprising the steps of: sensing excitation energy resulting from naturally occurring radiant flux density applied by natural light to the predetermined parcel of water and existing in the parcel of water over a period of time; producing an electrical signal representative of the sensed naturally occurring excitation energy; sensing the naturally occurring fluorescent emission from within the predetermined parcel of water which emission exists in the parcel of water over a period of time as a result of the existing naturally occurring applied excitation energy; producing an electrical signal representative of the sensed fluorescent emission; combining the electrical signals representative of sensed naturally occurring excitation energy and sensed fluorescent emission; and calculating the rate of primary production in the predetermined parcel of water from said electrical signals.
Claim 3
Dependent Claim
82 words
The method recited in claim 1, and comprising the further steps of: calculating fluorescence per unit volume of water from said step of producing a signal representative of sensed fluorescent emission; producing an electrical signal representative of the calculated fluorescence per unit volume; and combining the electrical signals representative of sensed naturally occurring excitation energy and fluorescence per unit volume; said primary production calculating step being the result of combining said signals representative of sensed excitation energy and fluorescence per unit volume.
Claim 4
Dependent Claim
31 words
The method recited in claim 2, and comprising the further step of computing daily primary production by integrating the rate of primary production over the period of at least one day.
Claim 5
Dependent Claim
89 words
The method recited in claim 2, and comprising the further steps of: sensing excitation energy applied to the surface of the water; producing an electrical signal representative of the sensed surface excitation energy; producing a signal representative of instantaneous rate of primary productioon in the predetermined parcel of water; combining the electrical signals representative of surface excitation energy and instantaneous rate of primary production with data as to time of day, day length and sun angle; and computing daily primary production from the signal combination including surface excitation energy.
Claim 6
Dependent Claim
44 words
The method recited in claim 4, and comprising the further steps of: calculating the attenuation coefficient of the parcel of water; producing an electrical signal representative of the attenuation coefficient; and applying the attenuation coefficient signal to said fluorescence per unit volume calculating step.
Claim 7
Dependent Claim
53 words
The method recited in claim 5, and comprising the further steps of: sensing the pressure in the parcel of water; calculating the depth of the parcel of water; producing a signal representative of said depth and; applying said depth signal to said attenuation coefficient calculating step to thereby further refine said attenuation coefficient.
Claim 8
Dependent Claim
28 words
The method recited in claim 5, wherein said attenuation coefficient is calculated from a combination of the signals representative of surface excitation energy and the sensed excitation energy.
Claim 9
Independent Claim
142 words
A method for determining chlorophyll concentration in a predetermined parcel of water, said method comprising the steps of: passively sensing fluorescent excitation energy resulting from naturally occurring radiant flux density applied by natural light to the predetermined parcel of water and existing in the parcel of water over a period of time; producing an electrical signal representative of the sensed naturally occurring excitation energy; sensing the naturally occurring fluorescent emission from within the predetermined parcel of water which emission exists in the parcel of water over a period of time as a result of the existing naturally occurring applied excitation energy; producing an electrical signal representative of the sensed fluorescent emission; combining the electrical signals representative of sensed naturally occurring excitation energy and sensed fluorescent emission; and calculating the concentration of chlorophyll in the predetermined parcel of water from said electrical signals
Claim 10
Independent Claim
142 words
A method for determining chlorophyll concentration in a predetermined parcel of water, said method comprising the steps of: passively sensing fluorescent excitation energy resulting from naturally occurring radiant flux density applied by natural light to the predetermined parcel of water and existing in the parcel of water over a period of time; producing an electrical signal representative of the sensed naturally occurring excitation energy; sensing the naturally occurring fluorescent emission from within the predetermined parcel of water which emission exists in the parcel of water over a period of time as a result of the existing naturally occurring applied excitation energy; producing an electrical signal representative of the sensed fluorescent emission; combining the electrical signals representative of sensed naturally occurring excitation energy and sensed fluorescent emission; and calculating the concentration of chlorophyll in the predetermined parcel of water from said electrical signals
Claim 11
Independent Claim
147 words
A method for for determining chlorophyll concentration in a predetermined parcel of water, said method comprising the steps of: sensing naturally occurring excitation energy applied to the surface of the water over a period of time; producing an electrical signal representative of the sensed surface excitation energy; calculating the attenuation coefficient of the parcel of wa1ter; producing an electrical signal representative of the attenuation coefficient; sensing the naturally occurring fluorescent emission from wit1hin the predetermined parcel of water which emission exists in the parcel of water over a period of time as a result of the existing naturally occurring applied excitation energy; producing an electrical signal representative of the sensed fluorescent emission; combining the attenuation coefficient signal with the sensed surface excitation energy and sensed fluorescent emission signals; and producing a signal representative of chlorophyll concentration in the parcel of water from said combination of three signals.
Claim 12
Independent Claim
147 words
A method for for determining chlorophyll concentration in a predetermined parcel of water, said method comprising the steps of: sensing naturally occurring excitation energy applied to the surface of the water over a period of time; producing an electrical signal representative of the sensed surface excitation energy; calculating the attenuation coefficient of the parcel of wa1ter; producing an electrical signal representative of the attenuation coefficient; sensing the naturally occurring fluorescent emission from wit1hin the predetermined parcel of water which emission exists in the parcel of water over a period of time as a result of the existing naturally occurring applied excitation energy; producing an electrical signal representative of the sensed fluorescent emission; combining the attenuation coefficient signal with the sensed surface excitation energy and sensed fluorescent emission signals; and producing a signal representative of chlorophyll concentration in the parcel of water from said combination of three signals.
Claim 13
Independent Claim
237 words
A method for determining and predicting rate of photosynthetic primary production driven by natural light in a predetermined parcel of water, said method comprising the steps of: sensing excitation energy resulting from naturally occurring radiant flux density applied by natural light to the predetermined parcel of water and existing in the parcel of water over a period of time; producing an electrical signal representative of the sensed excitation energy; sensing the naturally occurring fluorescent emission from within the predetermined parcel of water which emission exists in the parcel of water over a period of time as a result of the existing naturally occurring applied excitation energy; producing an electrical signal representative of the sensed fluorescent emission; sensing irradiance energy level above the surface of the water in which the predetermined parcel resides; producing an electrical signal to correct for other levels of surface irradiance energy representative of the sensed surface irradiance energy; combining the electrical signals representative of sensed excitation energy and sensed fluorescent emission; calculating the rate of primary production in the predetermined parcel of water from said two combined electrical signals; further applying the other level of surface irradiance energy electrical signal to the calculated rate of primary production and calculating the predicted rate of primary production; and providing an output electrical signal representative of calculated and predicted rate of primary production under other naturally occurring conditions of irradiance in the predetermined parcel of water.
Claim 14
Independent Claim
237 words
A method for determining and predicting rate of photosynthetic primary production driven by natural light in a predetermined parcel of water, said method comprising the steps of: sensing excitation energy resulting from naturally occurring radiant flux density applied by natural light to the predetermined parcel of water and existing in the parcel of water over a period of time; producing an electrical signal representative of the sensed excitation energy; sensing the naturally occurring fluorescent emission from within the predetermined parcel of water which emission exists in the parcel of water over a period of time as a result of the existing naturally occurring applied excitation energy; producing an electrical signal representative of the sensed fluorescent emission; sensing irradiance energy level above the surface of the water in which the predetermined parcel resides; producing an electrical signal to correct for other levels of surface irradiance energy representative of the sensed surface irradiance energy; combining the electrical signals representative of sensed excitation energy and sensed fluorescent emission; calculating the rate of primary production in the predetermined parcel of water from said two combined electrical signals; further applying the other level of surface irradiance energy electrical signal to the calculated rate of primary production and calculating the predicted rate of primary production; and providing an output electrical signal representative of calculated and predicted rate of primary production under other naturally occurring conditions of irradiance in the predetermined parcel of water.
Claim 15
Dependent Claim
80 words
The method recited in claim 10, and comprising the further steps of: calculating fluorescence per unit volume of water from said step of producing a signal representative of sensed fluorescent emissions; producing an electrical signal representative of the calculated fluorescence per unit volume; and combining the electrical signals representative of sensed excitation energy and fluorescence per unit volume; said primary production calculating step being the result of combining said signals representative of sensed excitation energy and fluorescence per unit volume.
Claim 16
Dependent Claim
31 words
The method recited in claim 11, and comprising the further step of computing daily primary production by integrating the rate of primary production over the period of at least one day.
Claim 17
Dependent Claim
68 words
The method recited in claim 11, and comprising the further steps of: producing a signal representative of instantaneous rate of primary production in the predetermined parcel of water; combining the electrical signals representative of surface irradiance energy and instantaneous rate of primary production with data as to time of day, day length and sun angle; and computing daily primary production from the signal combination including surface irradiance energy.
Claim 18
Dependent Claim
44 words
The method recited in claim 13, and comprising the further steps of: calculating the attenuation coefficient of the parcel of water; producing an electrical signal representative of the attenuation coefficient; and applying the attenuation coefficient signal to said fluorescence per unit volume calculating step.
Claim 19
Dependent Claim
52 words
The method recited in claim 14, and comprising the further steps of: sensing the pressure in the parcel of water; calculating the depth of the parcel of water; producing a signal representative of said depth; applying said depth signal to said attenuation coefficient calculating step to thereby further refine said attenuation coefficient.
Claim 20
Dependent Claim
29 words
The method recited in claim 14, wherein said attenuation coefficient is calculated from a combination of the signals representative of surface irradiance energy and the sensed fluorescent excitation energy.
Claim 21
Independent Claim
105 words
A method for determining the natural rate of photosynthetic primary production driven by natural light in a predetermined parcel of water, said method comprising these steps of: sensing the naturally occurring fluorescent emission from within the predetermined parcel of water which emission exists in the parcel of water over a period of time as a result of being driven by natural light; producing an electrical signal representative of the sensed fluorescent emission; calculating the rate of primary production in the predetermined parcel of water from said electrical signal; and producing a signal representative of the instantaneous rate of primary production in the parcel of water.
Claim 22
Independent Claim
105 words
A method for determining the natural rate of photosynthetic primary production driven by natural light in a predetermined parcel of water, said method comprising these steps of: sensing the naturally occurring fluorescent emission from within the predetermined parcel of water which emission exists in the parcel of water over a period of time as a result of being driven by natural light; producing an electrical signal representative of the sensed fluorescent emission; calculating the rate of primary production in the predetermined parcel of water from said electrical signal; and producing a signal representative of the instantaneous rate of primary production in the parcel of water.
Claim 23
Dependent Claim
31 words
The method recited in claim 17, and comprising the further step of computing daily primary production by integrating the rate of primary production over the period of at least one day.
Claim 24
Dependent Claim
63 words
The method recited in claim 17, and comprising the further steps of: sensing the pressure in the parcel of water; calculating the depth of the parcel of water; producing a signal representative of said depth; and applying said depth signal to the signal representative of instantaneous rate of primary production to thereby further refine said signal representative of instantaneous rate of primary production.
Claim 25
Independent Claim
189 words
A method for determining the natural rate of photosynthetic primary production and chlorophyll concentration driven by natural light in a predetermined parcel of water, said method comprising the steps of: sensing excitation energy resulting from naturally occurring radiant flux density applied by natural light to the predetermined parcel of water and existing in the parcel of water over a period of time; producing an electrical signal representative of the sensed naturally occurring excitation energy; sensing the naturally occurring fluorescent emission from within the predetermined parcel of water which emission exists in the parcel of water over a period of time as a result of the existing naturally occurring applied excitation energy; producing an electrical signal representative of the sensed fluorescent emission; combining the electrical signals representative of sensed excitation energy and sensed fluorescent emission; calculating the rate of primary production and concentration of chlorophyll in the predetermined parcel of water from said electrical signals; and providing a first output electrical signal representative of calculated primary production in the predetermined parcel of water, and a second output electrical signal representative of calculated chlorophyll concentration in the predetermined parcel of water.
Claim 26
Independent Claim
189 words
A method for determining the natural rate of photosynthetic primary production and chlorophyll concentration driven by natural light in a predetermined parcel of water, said method comprising the steps of: sensing excitation energy resulting from naturally occurring radiant flux density applied by natural light to the predetermined parcel of water and existing in the parcel of water over a period of time; producing an electrical signal representative of the sensed naturally occurring excitation energy; sensing the naturally occurring fluorescent emission from within the predetermined parcel of water which emission exists in the parcel of water over a period of time as a result of the existing naturally occurring applied excitation energy; producing an electrical signal representative of the sensed fluorescent emission; combining the electrical signals representative of sensed excitation energy and sensed fluorescent emission; calculating the rate of primary production and concentration of chlorophyll in the predetermined parcel of water from said electrical signals; and providing a first output electrical signal representative of calculated primary production in the predetermined parcel of water, and a second output electrical signal representative of calculated chlorophyll concentration in the predetermined parcel of water.
Claim 27
Dependent Claim
81 words
The method recited in claim 20, and comprising the further steps of: calculating fluorescence per unit volume of water from said step of producing a signal representative of sensed fluorescent emission; producing an electrical signal representative of the calculated fluorescence per unit volume; and combining the electrical signals representative of the sensed excitation energy and fluorescence per unit volume; said primary production calculating step being the result of combining said signals representative of sensed excitation energy and fluorescence per unit volume.
Claim 28
Dependent Claim
31 words
The method recited in claim 21, and comprising the further step of computing daily primary production by integrating the rate of primary production over the period of at least one day.
Claim 29
Dependent Claim
89 words
The method recited in claim 21, and comprising the further steps of: sensing excitation energy applied to the surface of the water; producing an electrical signal representative of the sensed surface excitation energy; producing a signal representative of instantaneous rate of primary production in the predetermined parcel of water; combining the electrical signals representative of surface excitation energy and instantaneous rate of primary production with data as to time of day, day length and sun angle; and computing daily primary production from the signal combination including surface excitation energy.
Claim 30
Dependent Claim
44 words
The method recited in claim 23, and comprising the further steps of: calculating the attenuation coefficient of the parcel of water; producing an electrical signal representative of the attenuation coefficient; and applying the attenuation coefficient signal to said fluorescence per unit volume calculating step.
Claim 31
Dependent Claim
52 words
The method recited in claim 24, and comprising the further steps of: sensing the pressure in the parcel of water; calculating the depth of the parcel of water; producing a signal representative of said depth; applying said depth signal to said attenuation coefficient calculating step to thereby further refine said attenuation coefficient.
Claim 32
Dependent Claim
46 words
The method recited in claim 25, and comprising the further steps of: combining the attenuation coefficient signal with the sensed excitation energy and sensed fluorescent emission signals; and producing a signal representative of chlorophyll concentration in the parcel of water from said combination of three signals.
Claim 33
Dependent Claim
28 words
The method recited in claim 24, wherein said attenuation coefficient is calculated from a combination of the signals representative of surface excitation energy and the sensed excitation energy.
Claim 34
Dependent Claim
175 words
Apparatus for determining the natural rate of photosynthetic primary production driven by natural light in a predetermined parcel of water, said apparatus comprising: first detector means for sensing the naturally occurring excitation energy resulting from naturally occurring radiant flux density applied by natural light in the predetermined parcel of water and existing in the parcel of water over a period of time; means connected to said first detector means for producing a first electrical signal representative of the detected naturally occurring excitation energy; second detector means for sensing the naturally occurring fluorescent emission from within the water in the predetermined parcel of water which emission exists in the parcel of water over a period of time as a result of the existing naturally occurring applied excitation energy; means connected to said second detector means for producing a second electrical signal representative of the detected fluorescent emission; and first computation means for combining said first and second electrical signals, said first computation means having an output providing a signal representative of the rate of primary production.
Claim 35
Dependent Claim
175 words
Apparatus for determining the natural rate of photosynthetic primary production driven by natural light in a predetermined parcel of water, said apparatus comprising: first detector means for sensing the naturally occurring excitation energy resulting from naturally occurring radiant flux density applied by natural light in the predetermined parcel of water and existing in the parcel of water over a period of time; means connected to said first detector means for producing a first electrical signal representative of the detected naturally occurring excitation energy; second detector means for sensing the naturally occurring fluorescent emission from within the water in the predetermined parcel of water which emission exists in the parcel of water over a period of time as a result of the existing naturally occurring applied excitation energy; means connected to said second detector means for producing a second electrical signal representative of the detected fluorescent emission; and first computation means for combining said first and second electrical signals, said first computation means having an output providing a signal representative of the rate of primary production.
Claim 36
Dependent Claim
40 words
The apparatus recited in claim 28, and further comprising: means connected to said second signal producing means for calculating fluorescence per unit volume of water and producing an electrical signal representative thereof, said signal being coupled to said computation means.
Claim 37
Dependent Claim
23 words
The apparatus recited in claim 29, and further comprising means connected to said output of said computation means for calculating daily primary production.
Claim 38
Dependent Claim
16 words
The apparatus recited in claim 30, wherein said daily primary production calculating means comprises integration means.
Claim 39
Dependent Claim
86 words
The apparatus recited in claim 30, and further comprising: third detector means for sensing irradiance energy level above the surface of the water in which said predetermined parcel resides; means connected to said third detector means for producing as an output a third electrical signal representative of said above surface irradiance energy level; the output signal from said computation means representing instantaneous rate of primary production; means combining said instantaneous rate output signal and said third signal and producing an output signal representing daily primary production.
Claim 40
Dependent Claim
58 words
The apparatus recited in claim 32, and further comprising: means connected to the outputs of said third signal producing means and said first signal producing means to produce a signal representing the attenuation coefficient of the parcel of water; said attenuation coefficient signal being connected to said fluorescence per unit volume calculating means to refine the output thereof.
Claim 41
Dependent Claim
71 words
The apparatus recited in claim 33, and further comprising: fourth detector means for sensing pressure and producing an output signal indicative thereof; means connected to said output of said fourth detector means for producing as an output a fourth electrical signal representative of depth of said fourth detector, said fourth electrical signal being connected to said attenuation coefficient signal producing means to enable said attenuation coefficient signal to be further refined.
Claim 42
Dependent Claim
41 words
The apparatus recited in claim 28, and further comprising: second computation means for combining said first and second electrical signals, said second computation means having an output providing a signal representative of the concentration of chlorophyll in the parcel of water.
Claim 43
Dependent Claim
18 words
The apparatus recited in claim 28, and further comprising means for visually indicating the rate of primary production.
Claim 44
Dependent Claim
22 words
The apparatus recited in claim 28, and further comprising means for recording said first and second electrical signals for later operation thereon.
Claim 45
Dependent Claim
21 words
The apparatus recited in claim 28, and further comprising means for recording said signals representing the calculated rate of primary production.
Claim 46
Dependent Claim
99 words
Apparatus for determining the natural rate of photosynthetic primary production driven by natural light at a predetermined level in a body of water, said apparatus comprising: detector means for sensing the naturally occurring fluorescent emission from within the water which emission exists at the predetermined level in the water over a period of time as a result of being driven by natural light; means for producing an electrical signal representative of the detected fluorescent emission; and computation means for providing a signal representative of the rate of primary production from the electrical signal representative of the detected fluorescent emission.
Claim 47
Dependent Claim
99 words
Apparatus for determining the natural rate of photosynthetic primary production driven by natural light at a predetermined level in a body of water, said apparatus comprising: detector means for sensing the naturally occurring fluorescent emission from within the water which emission exists at the predetermined level in the water over a period of time as a result of being driven by natural light; means for producing an electrical signal representative of the detected fluorescent emission; and computation means for providing a signal representative of the rate of primary production from the electrical signal representative of the detected fluorescent emission.
Claim 48
Independent Claim
232 words
A method for determining rate of primary production in a predetermined parcel of water, said method comprising the steps of: sensing excitation energy applied to the predetermined parcel of water; producing an electrical signal representative of the sensed excitation energy; sensing fluorescent emission from within the predetermined parcel of water; producing an electrical signal representative of the sensed fluorescent emission; combining the electrical signals representative of sensed excitation energy and sensed fluorescent emission; calculating the rate of primary production in the predetermined parcel of water from said electrical signals; calculating fluorescence per unit volume of water from said step of producing a signal of sensed fluorescent emission; producing an electrical signal representative of the calculated fluorescence per unit volume; combining the electrical signals representative of sensed excitation energy and fluorescence per unit volume; said primary production calculating step being the result of combining said signals representative of sensed excitation energy and fluorescence per unit volume; sensing excitation energy applied to the surface of the water; producing an electrical signal representative of the sensed surface excitation energy; producing a signal representative of instantaneous rate of primary production in the predetermined parcel of water; combining the electrical signals representative of surface excitation energy and instantaneous rate of primary production with data as to time of day, day length and sun angle; and computing daily primary production from the signal combination including surface excitation energy.
Claim 49
Independent Claim
232 words
A method for determining rate of primary production in a predetermined parcel of water, said method comprising the steps of: sensing excitation energy applied to the predetermined parcel of water; producing an electrical signal representative of the sensed excitation energy; sensing fluorescent emission from within the predetermined parcel of water; producing an electrical signal representative of the sensed fluorescent emission; combining the electrical signals representative of sensed excitation energy and sensed fluorescent emission; calculating the rate of primary production in the predetermined parcel of water from said electrical signals; calculating fluorescence per unit volume of water from said step of producing a signal of sensed fluorescent emission; producing an electrical signal representative of the calculated fluorescence per unit volume; combining the electrical signals representative of sensed excitation energy and fluorescence per unit volume; said primary production calculating step being the result of combining said signals representative of sensed excitation energy and fluorescence per unit volume; sensing excitation energy applied to the surface of the water; producing an electrical signal representative of the sensed surface excitation energy; producing a signal representative of instantaneous rate of primary production in the predetermined parcel of water; combining the electrical signals representative of surface excitation energy and instantaneous rate of primary production with data as to time of day, day length and sun angle; and computing daily primary production from the signal combination including surface excitation energy.
Claim 50
Dependent Claim
44 words
The method recited in claim 40, and comprising the further steps of: calculating the attenuation coefficient of the parcel of water; producing an electrical signal representative of the attenuation coefficient; and applying the attenuation coefficient signal to said fluorescence per unit volume calculating step.
Claim 51
Dependent Claim
53 words
The method recited in claim 41, and comprising the further steps of: sensing the pressure in the parcel of water; calculating the depth of the parcel of water; producing a signal representative of said depth; and applying said depth signal to said attenuation coefficient calculating step to thereby further refine said attenuation coefficient.
Claim 52
Dependent Claim
28 words
The method recited in claim 41, wherein said attenuation coefficient is calculated from a combination of the signals representative of surface excitation energy and the sensed excitation energy.
Claim 53
Independent Claim
279 words
A method for determining and predicting rate of primary production in a predetermined parcel of water, said method comprising the steps of: sensing fluorescent excitation energy applied to the predetermined parcel of water; producing an electrical signal representative of the sensed excitation energy; sensing fluorescent emission from within the predetermined parcel of water; producing an electrical signal representative of the sensed fluorescent emission; sensing irradiance energy level above the surface of the water in which the predetermined parcel resides; producing a corrective electrical signal representative of the sensed surface irradiance energy; combining the electrical signals representative of sensed excitation energy and sensed fluorescent emission; calculating the rate of primary production in the predetermined parcel of water from said two combined electrical signals; further applying the corrective electrical signal to said two combined signals and calculating the predicted rate of primary production; providing an output electrical signal representative of calculated and predicted rate of primary production in the predetermined parcel of water; calculating fluorescence per unit volume of water from said sensed fluorescent emission producing step; producing an electrical signal representative of the calculated fluorescence per unit volume; combining the electrical signals representative of sensed excitation energy and fluorescence per unit volume; said primary production calculating step being the result of combining said signals representative of sensed excitation energy and fluorescence per unit volume; producing a signal representative of instantaneous rate of primary production in the predetermined parcel of water; combining the electrical signals representative of surface irradiance energy and instantaneous rate of primary production with data as to time of day, day length and sun angle; and computing daily primary production from the signal combination including surface excitation energy.
Claim 54
Independent Claim
279 words
A method for determining and predicting rate of primary production in a predetermined parcel of water, said method comprising the steps of: sensing fluorescent excitation energy applied to the predetermined parcel of water; producing an electrical signal representative of the sensed excitation energy; sensing fluorescent emission from within the predetermined parcel of water; producing an electrical signal representative of the sensed fluorescent emission; sensing irradiance energy level above the surface of the water in which the predetermined parcel resides; producing a corrective electrical signal representative of the sensed surface irradiance energy; combining the electrical signals representative of sensed excitation energy and sensed fluorescent emission; calculating the rate of primary production in the predetermined parcel of water from said two combined electrical signals; further applying the corrective electrical signal to said two combined signals and calculating the predicted rate of primary production; providing an output electrical signal representative of calculated and predicted rate of primary production in the predetermined parcel of water; calculating fluorescence per unit volume of water from said sensed fluorescent emission producing step; producing an electrical signal representative of the calculated fluorescence per unit volume; combining the electrical signals representative of sensed excitation energy and fluorescence per unit volume; said primary production calculating step being the result of combining said signals representative of sensed excitation energy and fluorescence per unit volume; producing a signal representative of instantaneous rate of primary production in the predetermined parcel of water; combining the electrical signals representative of surface irradiance energy and instantaneous rate of primary production with data as to time of day, day length and sun angle; and computing daily primary production from the signal combination including surface excitation energy.
Claim 55
Dependent Claim
44 words
The method recited in claim 44, and comprising the further steps of: calculating the attenuation coefficient of the parcel of water; producing an electrical signal representative of the attenuation coefficient; and applying the attenuation coefficient signal to said fluorescence per unit volume calculating step.
Claim 56
Dependent Claim
53 words
The method recited in claim 45, and comprising the further steps of: sensing the pressure in the parcel of water; calculating the depth of the parcel of water; producing a signal representative of said depth; and applying said depth signal to said attenuation coefficient calculating step to thereby further refine said attenuation coefficient.
Claim 57
Dependent Claim
29 words
The method recited in claim 45, wherein said attenuation coefficient is calculated from a combination of the signals representative of surface irradiance energy and the sensed fluorescent excitation energy.
Claim 58
Independent Claim
269 words
A method for determining rate of primary production and chlorophyll concentration in a predetermined parcel of water, said method comprising the steps of: sensing excitation energy applied to the predetermined parcel of water; producing an electrical signal representative of the sensed excitation energy; sensing fluorescent emission from within the predetermined parcel of water; producing an electrical signal representative of the sensed fluorescent emission; combining the electrical signals representative of sensed excitation energy and sensed fluorescent emission; calculating the rate of primary production and concentration of chlorophyll in the predetermined parcel of water from said electrical signals; providing a first output electrical signal representative of calculated primary production in the predetermined parcel of water, and a second output electrical signal representative of calculated chlorophyll concentration in the predetermined parcel of water; calculating fluorescence per unit volume of water from said sensed fluorescent emission producing step; producing an electrical signal representative of the calculated fluorescence per unit volume; combining the electrical signals representative of sensed excitation energy and fluorescence per unit volume; said primary production calculating step being the result of combining said signals representative of sensed excitation energy and fluorescence per unit volume; sensing excitation energy applied to the surface of the water; producing an electrical signal representative of the sensed surface excitation energy; producing a signal representative of instantaneous rate of primary production in the predetermined parcel of water; combining the electrical signals representative of surface excitation energy and instantaneous rate of primary production with data as to time of day, day length and sun angle; and computing daily primary production from the signal combination including surface excitation energy.
Claim 59
Independent Claim
269 words
A method for determining rate of primary production and chlorophyll concentration in a predetermined parcel of water, said method comprising the steps of: sensing excitation energy applied to the predetermined parcel of water; producing an electrical signal representative of the sensed excitation energy; sensing fluorescent emission from within the predetermined parcel of water; producing an electrical signal representative of the sensed fluorescent emission; combining the electrical signals representative of sensed excitation energy and sensed fluorescent emission; calculating the rate of primary production and concentration of chlorophyll in the predetermined parcel of water from said electrical signals; providing a first output electrical signal representative of calculated primary production in the predetermined parcel of water, and a second output electrical signal representative of calculated chlorophyll concentration in the predetermined parcel of water; calculating fluorescence per unit volume of water from said sensed fluorescent emission producing step; producing an electrical signal representative of the calculated fluorescence per unit volume; combining the electrical signals representative of sensed excitation energy and fluorescence per unit volume; said primary production calculating step being the result of combining said signals representative of sensed excitation energy and fluorescence per unit volume; sensing excitation energy applied to the surface of the water; producing an electrical signal representative of the sensed surface excitation energy; producing a signal representative of instantaneous rate of primary production in the predetermined parcel of water; combining the electrical signals representative of surface excitation energy and instantaneous rate of primary production with data as to time of day, day length and sun angle; and computing daily primary production from the signal combination including surface excitation energy.
Claim 60
Dependent Claim
44 words
The method recited in claim 48, and comprising the further steps of: calculating the attenuation coefficient of the parcel of water; producing an electrical signal representative of the attenuation coefficient; and applying the attenuation coefficient signal to said fluorescence per unit volume calculating step.
Claim 61
Dependent Claim
53 words
The method recited in claim 49, and comprising the further steps of: sensing the pressure in the parcel of water; calculating the depth of the parcel of water; producing a signal representative of said depth; and applying said depth signal to said attenuation coefficient calculating step to thereby further refine said attenuation coefficient.
Claim 62
Dependent Claim
46 words
The method recited in claim 50, and comprising the further steps of: combining the attenuation coefficient signal with the sensed excitation energy and sensed fluorescent emission signals; and producing a signal representative of chlorophyll concentration in the parcel of water from said combination of three signals.
Claim 63
Dependent Claim
28 words
The method recited in claim 49, wherein said attenuation coefficient is calculated from a combination of the signals representative of surface excitation energy and the sensed excitation energy.
Claim 64
Dependent Claim
283 words
Apparatus for determining rate of primary production in a predetermined parcel of water, said apparatus comprising: first detector means for sensing the excitation energy applied in the predetermined parcel of water; means connected to said first detector means for producing a first electrical signal representative of the detected excitation energy; second detector means for sensing fluorescent emission from within the predetermined parcel of water; means connected to said second detector means for producing a second electrical signal representative of the detected fluorescent emission; first computation means for combining said first and second electrical signals, said first computation means having an output providing a signal representative of the rate of primary production; means connected to said second signal producing means for calculating fluorescence per unit volume of water and producing an electrical signal representative thereof, said signal being coupled to said computation means; means connected to said output of said computation means for calculating daily primary production; third detector means for sensing irradiance energy level above the surface of the water in which said predetermined parcel resides; means connected to said third detector means for producing as an output a third electrical signal representative of said above surface irradiance energy level; the output signal from said computation means representing instantaneous rate of primary production; means combining said instantaneous rate output signal and said third signal and producing an output signal representing daily primary production; and means connected to the outputs of said third signal producing means and said first signal producing means to produce a signal representing the attenuation coefficient of the parcel of water; said attenuation coefficient signal being connected to said fluorescence per unit volume calculating means to refine the output thereof.
Claim 65
Dependent Claim
283 words
Apparatus for determining rate of primary production in a predetermined parcel of water, said apparatus comprising: first detector means for sensing the excitation energy applied in the predetermined parcel of water; means connected to said first detector means for producing a first electrical signal representative of the detected excitation energy; second detector means for sensing fluorescent emission from within the predetermined parcel of water; means connected to said second detector means for producing a second electrical signal representative of the detected fluorescent emission; first computation means for combining said first and second electrical signals, said first computation means having an output providing a signal representative of the rate of primary production; means connected to said second signal producing means for calculating fluorescence per unit volume of water and producing an electrical signal representative thereof, said signal being coupled to said computation means; means connected to said output of said computation means for calculating daily primary production; third detector means for sensing irradiance energy level above the surface of the water in which said predetermined parcel resides; means connected to said third detector means for producing as an output a third electrical signal representative of said above surface irradiance energy level; the output signal from said computation means representing instantaneous rate of primary production; means combining said instantaneous rate output signal and said third signal and producing an output signal representing daily primary production; and means connected to the outputs of said third signal producing means and said first signal producing means to produce a signal representing the attenuation coefficient of the parcel of water; said attenuation coefficient signal being connected to said fluorescence per unit volume calculating means to refine the output thereof.
Claim 66
Dependent Claim
71 words
The apparatus recited in claim 53, and further comprising: fourth detector means for sensing pressure and producing an output signal indicative thereof; means connected to said output of said fourth detector means for producing as an output a fourth electrical signal representative of depth of said fourth detector, said fourth electrical signal being connected to said attenuation coefficient signal producing means to enable said attenuation coefficient signal to be further refined.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-02-14 | 1992-08-15 | 1993-02-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-02-14 | 1996-08-15 | 1997-02-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-02-14 | 2000-08-15 | 2001-02-14 | $7700.00 | Window Closed / Lapsed |