Use of polymeric mordants to increase the intensity of rigid fluorescent dyes

Claim 2

An analytical dye composition comprising a rigid phenalenone, benzphenalenone or 4-oxo-4H-benz-[d,e]anthracene fluorescent dye or a hydrolyzable or reducible precursor thereof which releases a rigid fluorescent dye upon hydrolysis or reduction, respectively, of said precursor and a cationic or nonionic polymeric mordant.

Claim 3

The composition of claim 1 wherein said fluorescent dye is a phenalenone or benzphenalenone dye.

Claim 4

The composition of claim 1 comprising a reducible fluorescent dye precursor of the structure CAR--R 1 ) n wherein CAR-- is an aromatic or quinone nucleus having one or more hydrogen atoms removed to provide one or more valences through which R 1 is attached to CAR--, R 1 comprises a phenalenone or benzphenalenone moiety derived from ##STR32## wherein R is hydroxy or mercapto, said moiety being attached to CAR-- through an oxy or thio linkage obtained by removal of hydrogen from R, and n is 1 or 2, provided that when R 1 is replaced with H, CAR--(H) n has an E 1/2 of either at least about +100 mV when measured in water.

Claim 5

The composition of claim 3 wherein said dye precursor has the structure CAR--R 1 , wherein CAR-- is ##STR33## R 1 is ##STR34## R 2 and R 4 are independently hydrogen, alkyl, aryl or an electron withdrawing group, R 3 is R 1 , hydrogen, alkyl, aryl or an electron withdrawing group, or R 3 and R 4 , taken together, represent the atoms necessary to complete a fused carbocyclic ring, R 5 is alkylene or 1 or 2 carbon atoms, R 6 is methyl, Q is carbonyl or thiocarbonyl, FRAG is said phenalenone or benzphenalenone moiety attached to Q through said oxy or thio linkage, and m is 0 or 1.

Claim 6

The composition of claim 4 wherein FRAG is ##STR35##

Claim 7

The composition of claim 1 comprising a cationic polymeric mordant.

Claim 8

The composition of claim 1 buffered at a pH of 9 or less.

Claim 9

A composition for the determination of a living organism, said composition comprising an electron transfer agent, a cationic or nonionic mordant, and a reducible fluorescent dye precursor of the structure CAR--R 1 ) n wherein CAR-- is an aromatic or quinone nucleus having one or more hydrogen atoms removed to provide one or more valences through which R 1 is attached to CAR--, R 1 is ##STR36## wherein R 5 is alkylene of 1 or 2 carbon atoms, R 6 is methyl, Q is carbonyl or thiocarbonyl and FRAG is a phenalenone or benzphenalenone moiety derived from ##STR37## wherein R is hydroxy or mercapto, said moiety being attached to Q through an oxy or thio linkage obtained by removal of hydrogen from R, and n is 1 or 2, provided said precursor is capable of being reduced at physiological pH.

Claim 10

A composition for the determination of a living organism, said composition comprising an electron transfer agent, a cationic or nonionic mordant, and a reducible fluorescent dye precursor of the structure CAR--R 1 ) n wherein CAR-- is an aromatic or quinone nucleus having one or more hydrogen atoms removed to provide one or more valences through which R 1 is attached to CAR--, R 1 is ##STR36## wherein R 5 is alkylene of 1 or 2 carbon atoms, R 6 is methyl, Q is carbonyl or thiocarbonyl and FRAG is a phenalenone or benzphenalenone moiety derived from ##STR37## wherein R is hydroxy or mercapto, said moiety being attached to Q through an oxy or thio linkage obtained by removal of hydrogen from R, and n is 1 or 2, provided said precursor is capable of being reduced at physiological pH.

Claim 11

The composition of claim 8 wherein said dye precursor has the structure CAR--R 1 , wherein CAR-- is ##STR38## R 2 and R 4 are indepenndently hydrogen, alkyl, aryl or an electron withdrawing group, R 3 is R 1 , hydrogen, alkyl, aryl or an electron withdrawing group, or R 3 and R 4 , taken together, represent the atoms necessary to complete a fused carbocyclic ring, and m is 0 or 1, provided that when R 1 is replaced with H, CAR--H has an E 1/2 of at least about +100 mV when measured in water.

Claim 12

The composition of claim 8 further comprising a surfactant.

Claim 13

A dry analytical element for the determination of an analyte comprising an absorbent carrier material, and containing, in association, a rigid phenalenone, benzphenalenone or 4-oxo-4H-benz-[d,e]-anthracene fluorescent dye or a hydrolyzable or reducible precursor thereof which releases a rigid fluorescent dye upon hydrolysis or reduction, respectively, of said precursor and a cationic or nonionic polymeric mordant.

Claim 14

A dry analytical element for the determination of an analyte comprising an absorbent carrier material, and containing, in association, a rigid phenalenone, benzphenalenone or 4-oxo-4H-benz-[d,e]-anthracene fluorescent dye or a hydrolyzable or reducible precursor thereof which releases a rigid fluorescent dye upon hydrolysis or reduction, respectively, of said precursor and a cationic or nonionic polymeric mordant.

Claim 15

The element of claim 11 wherein said fluorescent dye precursor is a reducible compound of the structure CAR--R 1 ) n wherein CAR-- is an aromatic or quinone nucleus, having one or more hydrogen atoms removed to provide one or more valences through which R 1 is attached to CAR--, R 1 is ##STR39## wherein R 5 is alkylene of 1 or 2 carbon atoms, R 6 is methyl, Q is carbonyl or thiocarbonyl and FRAG is a phenalenone or benzphenalenone moiety derived from ##STR40## wherein R is hydroxy or mercapto, said moiety being attached to Q through an oxy or thio linkage obtained by removal of hydrogen from R, and n is 1 or 2.

Claim 16

The element of claim 12 wherein said dye precursor has the structure CAR--R 1 , wherein CAR-- is ##STR41## R 2 and R 4 are independently hydrogen, alkyl, aryl or an electron withdrawing group, R 3 is R 1 , hydrogen, alkyl, aryl or an electron withdrawing group, or R 3 and R 4 , taken together, represent the atoms necessary to complete a fused carbocyclic ring, and m is 0 or 1, provided that when R 1 is replaced with H, CAR--H has an E 1/2 of at least about +100 mV when measured in water.

Claim 17

The element of claim 13 wherein FRAG is a phenalenone moiety.

Claim 18

The element of claim 11 further comprising an interactive composition for said analyte.

Claim 19

The element of claim 11 further comprising an electron transfer agent.

Claim 20

The element of claim 11 further comprising a support carrying said absorbent carrier material.

Claim 23

The method of claim 18 wherein said fluorescent dye precursor is a reducible compound of the structure CAR--R 1 ) n wherein CAR-- is an aromatic or quinone nucleus having one or more hydrogen atoms removed to provide one or more valences through which R 1 is attached to CAR--, R 1 is ##STR42## wherein R 5 is alkylene of 1 or 2 carbon atoms, R 6 is methyl, Q is carbonyl or thiocarbonyl and FRAG is a phenalenone or benzphenalenone moiety derived from ##STR43## wherein R is hydroxy or mercapto, said moiety being attached to Q through an oxy or thio linkage obtained by removal of hydrogen from R, and n is 1 or 2.

Claim 24

The method of claim 19 wherein said dye precursor has the structure CAR--R 1 , wherein CAR-- is ##STR44## R 2 and R 4 are independently hydrogen, alkyl, aryl or an electron withdrawing group, R 3 is R 1 , hydrogen, alkyl, aryl or an electron withdrawing group, or R 3 and R 4 , taken together, represent the atoms necessary to complete a fused carbocyclic ring, and m is 0 or 1, provided that when R 1 is replaced with H, CAR--H has an E 1/2 of at least about +100 mV when measured in water.

Claim 25

The method of claim 18 for the determination of a living organism.

Claim 26

The method of claim 18 wherein said contacting is carried out in the presence of an electron transfer agent.

Claim 27

The method of claim 18 for the determination of a nonliving substance in the presence of an interactive composition for said analyte.