US4803155A
Expired / Lapsed
US
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Monoclonal antibodies to Sclerotinia homoeocarpa
Assignee: NOEGEN Corp
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Filing: 1986-02-27
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Granted: 1989-02-07
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Expires: 2006-02-27
Abstract
Abstract This invention provides monoclonal antibodies which specifically bind to Sclerotinia homoeocarpa the causative agent of dollarspot disease in plants. Hybridoma producing the antibodies as well as materials and kits for carrying out the detection of Sclerotina homoeocarpa are also disclosed.
Inventors
- 👤 Frank P. Petersen
- 👤 Adrianna M. Maybroda
- 👤 Sally A. Miller
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 04:16
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/14; Y; Y10; Y10S; Y10S435/00; Y10S435/81; Y10S435/8215; Y10S435/911; Y10S436/00; Y10S436/807; Y10S436/808
Claims Analysis
Total Claims: 26
Claim 1
Independent Claim
13 words
A monoclonal antibody capable of specifically binding with an antigen from Sclerotinia homoeocarpa.
Claim 2
Dependent Claim
13 words
A monoclonal antibody capable of specifically binding with an antigen from Sclerotinia homoeocarpa.
Claim 3
Dependent Claim
13 words
The antibody of claim 1 wherein the antibody is of the IgG class.
Claim 4
Dependent Claim
19 words
A hybridoma which produces a monoclonal antibody which is capable of specifically binding with an antigen from Sclerotinia homoeocarpa.
Claim 5
Dependent Claim
19 words
A hybridoma which produces a monoclonal antibody which is capable of specifically binding with an antigen from Sclerotinia homoeocarpa.
Claim 6
Dependent Claim
14 words
The hydridoma of claim 3 wherein said monoclonal antibody is of the IgG class.
Claim 7
Dependent Claim
14 words
The hybridoma of claim 4 wherein said monoclonal antibody is of the IgGl class.
Claim 8
Dependent Claim
15 words
The hybridoma of claim 5 having all of the identifying characteristics of ATCC HB 8944.
Claim 9
Dependent Claim
11 words
A monoclonal antibody produced by culturing the hybridoma of claim 3.
Claim 10
Dependent Claim
11 words
A monoclonal antibody produced by culturing the hybridoma of claim 4.
Claim 11
Dependent Claim
11 words
A monoclonal antibody produced by culturing the hybridoma of claim 5.
Claim 12
Dependent Claim
10 words
A monoclonal antibody produced by the hybridoma of claim 6.
Claim 13
Dependent Claim
16 words
The monoclonal antibody of claim 10 wherein said antibody is labelled with an analytically detectable reagent.
Claim 14
Dependent Claim
26 words
The monoclonal antibody of claim 11 wherein said reagent is selected from the group consisting of biotin, a fluorensent dye, a radioactive material and an enzyme.
Claim 15
Dependent Claim
15 words
The monoclonal antibody of claim 10 wherein said antibody is immobilized onto a solid support.
Claim 16
Independent Claim
78 words
A method or detecting the presence of an antigen from Sclerotinia homoeocarpa in a sample containing the antigen comprising: forming a binary complex between said antigen and a first antibody capable of reacting with said antigen; forming a tertiary complex by contacting the binary complex with a second monoclonal antibody; detecting the presence of said tertiary complex by contacting the tertiary complex with an analytically detectable immunological reagent thereby detecting the presence of the antigen from Sclerotinia homoeocarpa.
Claim 17
Independent Claim
78 words
A method or detecting the presence of an antigen from Sclerotinia homoeocarpa in a sample containing the antigen comprising: forming a binary complex between said antigen and a first antibody capable of reacting with said antigen; forming a tertiary complex by contacting the binary complex with a second monoclonal antibody; detecting the presence of said tertiary complex by contacting the tertiary complex with an analytically detectable immunological reagent thereby detecting the presence of the antigen from Sclerotinia homoeocarpa.
Claim 18
Dependent Claim
14 words
The method according to claim 14 wherein said first antibody is monoclonal or polyclonal.
Claim 19
Dependent Claim
16 words
The method according to claim 15 wherein said first antibody is immobilized to a solid support.
Claim 20
Dependent Claim
22 words
The method according to claim 24 wherein said analytically detectable immunological reagent is a third antibody reactive to said second monoclonal antibody.
Claim 21
Dependent Claim
30 words
The method according to claim 17 wherein said detection is by means of a label selected from the group consisting of biotin, a radioactive material, fluorescent dyes, and an enzyme.
Claim 22
Dependent Claim
20 words
The method according to claim 18 wherein the label is biotin and its presence is indicated by reaction with avidin.
Claim 23
Dependent Claim
26 words
The method according to claim 19 wherein said avidin is complexed with an enzyme and said complex is detected by measuring of the enzyme reaction product.
Claim 24
Dependent Claim
49 words
A kit for the immunological diagnosis of Sclerotinia homoeocarpa infection of plants comprising a carrier being compartmented to receive in close confinement therein an antigen extraction means; a solid support having affixed thereto a monoclonal antibody capable of specifically binding with Sclerotinia antigen; and a binary complex detecting mean.
Claim 25
Dependent Claim
49 words
A kit for the immunological diagnosis of Sclerotinia homoeocarpa infection of plants comprising a carrier being compartmented to receive in close confinement therein an antigen extraction means; a solid support having affixed thereto a monoclonal antibody capable of specifically binding with Sclerotinia antigen; and a binary complex detecting mean.
Claim 26
Dependent Claim
19 words
The kit according to claim 21 wherein said binary complex detecting means comprises a biotin-avidin enzyme linked immunoassay system.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-02-07 | 1992-08-08 | 1993-02-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-02-07 | 1996-08-08 | 1997-02-07 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-02-07 | 2000-08-08 | 2001-02-07 | $7700.00 | Window Closed / Lapsed |