US4802101A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Signal processing method for determining base sequence of nucleic acid
Assignee: Fujifilm Holdings Corp
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Filing: 1986-08-19
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Granted: 1989-01-31
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Expires: 2006-08-19
Abstract
Abstract A signal processing method for determining base sequence of nucleic acids by subjecting digital signals to signal processing, said digital signals corresponding to an autoradiograph of a resolved pattern which is formed by resolving a mixture of base-specific DNA fragments or base-specific RNA fragments labeled with a radioactive element in one-dimensional direction on a support medium, which comprises steps of: (1) preparing at least two one-dimensional waveforms for each band, with position along the resolving direction as abscissa and signal level as ordinate; (2) detecting positions at which signal level is maximum on each waveform; and (3) comparing the positions having maximum signal level detected on the plural waveforms for each band to determine a position of said band.
Inventors
- 👤 Makoto Hara
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 22:52
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6869; C12Q1/6874; G; G01; G01T; G01T1/00; G01T1/29; G01T1/2914; G01T1/2921; G01T1/2942; Y; Y10; Y10S; Y10S250/00; Y10S250/909; Y10T; Y10T436/00; Y10T436/14; Y10T436/142222; Y10T436/143333
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
164 words
A signal processing method for determining base sequence of nucleic acids by subjecting digital signals to signal processing, said digital signals corresponding to an autoradiograph of a resolved pattern which is formed by resolving a mixture of base-specific DNA fragments or base-specific RNA fragments labeled with a radioactive element in one-dimensional direction on a support medium, bands of the fragments desirably extending perpendicular to the one-dimensional direction of a row of said bands, which comprises steps of: (1) preparing at least two one-dimensional waveforms for each band where each waveform is substantially parallel in direction to said row, with position along the resolving direction as abscissa and signal level as ordinate; (2) detecting positions at which signal level is maximum on each waveform; (3) comparing the positions having maximum signal level detected on the plural waveforms for each band to determine a position of said band ; and (4) utilizing said position of said band to determine said base sequence of said nucleic acids.
Claim 2
Dependent Claim
164 words
A signal processing method for determining base sequence of nucleic acids by subjecting digital signals to signal processing, said digital signals corresponding to an autoradiograph of a resolved pattern which is formed by resolving a mixture of base-specific DNA fragments or base-specific RNA fragments labeled with a radioactive element in one-dimensional direction on a support medium, bands of the fragments desirably extending perpendicular to the one-dimensional direction of a row of said bands, which comprises steps of: (1) preparing at least two one-dimensional waveforms for each band where each waveform is substantially parallel in direction to said row, with position along the resolving direction as abscissa and signal level as ordinate; (2) detecting positions at which signal level is maximum on each waveform; (3) comparing the positions having maximum signal level detected on the plural waveforms for each band to determine a position of said band ; and (4) utilizing said position of said band to determine said base sequence of said nucleic acids.
Claim 3
Dependent Claim
49 words
The signal processing method as claimed in claim 1, wherein said digital signals are obtained by scanning the autoradiograph in such a manner that each band is traversed by at least two scanning lines, and in said step (1), the one-dimensional waveforms are prepared for the individual scanning lines.
Claim 4
Dependent Claim
36 words
The signal processing method as claimed in claim 1, wherein said position of the band is determined as an average of the positions having maximum signal level detected on the plural waveforms, in said step (3).
Claim 5
Dependent Claim
37 words
The signal processing method as claimed in claim 1, wherein said position of the band is determined as the greatest one of the positions having maximum signal level detected on the plural waveforms, in said step (3).
Claim 6
Dependent Claim
37 words
The signal processing method as claimed in claim 1, wherein said position of the band is determined as the least one of the positions having maximum signal level detected on the plural waveforms, in said step (3).
Claim 7
Dependent Claim
65 words
The signal processing method as claimed in claim 1, wherein the mixture of the base-specific DNA fragments consists of the four groups of: (1) guanine-specific DNA fragments; (2) adenine-specific DNA fragments; (3) thymine-specific DNA fragments; and (4) cytosine-specific DNA fragments; and the resolved pattern consists of four rows formed by resolving each of said four groups of the base-specific DNA fragments on the support medium.
Claim 8
Dependent Claim
67 words
The signal processing method as claimed in claim 1, wherein said digital signals corresponding to the autoradiograph are obtained by placing the support medium and a stimulable phosphor sheet comprising a stimulable phosphor together in layers to record the autoradiograph of the resolved pattern on the phosphor sheet as an energy-stored image, irradiating said phosphor sheet with stimulating rays and photoelectrically detecting the autoradiograph as stimulated emission.
Claim 9
Dependent Claim
58 words
The signal processing method as claimed in claim 1, wherein said digital signals corresponding to the autoradiograph are obtained by placing the support medium and a radiosensitive material together in layers to record the autoradiograph of the resolved pattern on the radiosensitive material as a visible image and photoelectrically reading out the autoradiograph visualized on said radiosensitive material.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-01-31 | 1992-08-01 | 1993-01-31 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-01-31 | 1996-08-01 | 1997-01-31 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-01-31 | 2000-08-01 | 2001-01-31 | $7700.00 | Window Closed / Lapsed |