US4797480A
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New biologically active fluorescent cyclic nucleotides
Assignee: University of Parma
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Filing: 1986-05-23
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Granted: 1989-01-10
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Expires: 2006-05-23
Abstract
Abstract Sybthesis of new biologically active fluorescent cyclic nucleotides of general formula: ##STR1## wherein X=OH and Y=NH 2 or X=NH 2 and Y=H and L is a fluorescent group of the class of 5 (or 6)-thioacetamido-fluorescein or of 5(or 8)-(2 thioacetamido-ethyl)-amino-naphthalene -1-sulphonic acid.
Inventors
- 👤 Robert T. Sorbi
- 👤 Antonio Caretta
- 👤 Andrea Cavaggioni
- 👤 Liliana Marchesi Gastaldi
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 05:33
CPC Classifications
C; C07; C07H; C07H19/00; C07H19/02; C07H19/04; C07H19/16; C07H19/20
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
55 words
Biologically active cyclic nucleotides made fluorescent by inserting a fluorescent group, represented by the formula: ##STR7## wherein: X is OH or NH 2 , Y is NH 2 when X=OH and is H when X=NH 2 and L is a fluorescent group selected from: 5-thioacetamido-fluorescein, 6-thioacetamido-fluorescein, 5-thioacetamido-2',4',5',7'-tetrabromofluorescein, 6-thioacetamido-2',4',5',7'-tetrabromofluorescein, 5-thioacetamido-2',4',5',7'-tetraiodo-fluorescein, 6-thioacetamido-2',4',5',7'-tetraiodofluorescein, 5-(2-thioacetamido-ethyl)-amino-naphthalene-1-sulphonic acid, and 8-(2-thioacetamido-ethyl)-amino-naphthalene-1-sulphonic acid.
Claim 2
Dependent Claim
55 words
Biologically active cyclic nucleotides made fluorescent by inserting a fluorescent group, represented by the formula: ##STR7## wherein: X is OH or NH 2 , Y is NH 2 when X=OH and is H when X=NH 2 and L is a fluorescent group selected from: 5-thioacetamido-fluorescein, 6-thioacetamido-fluorescein, 5-thioacetamido-2',4',5',7'-tetrabromofluorescein, 6-thioacetamido-2',4',5',7'-tetrabromofluorescein, 5-thioacetamido-2',4',5',7'-tetraiodo-fluorescein, 6-thioacetamido-2',4',5',7'-tetraiodofluorescein, 5-(2-thioacetamido-ethyl)-amino-naphthalene-1-sulphonic acid, and 8-(2-thioacetamido-ethyl)-amino-naphthalene-1-sulphonic acid.
Claim 3
Dependent Claim
13 words
The compound of claim 1 wherein X=OH, Y=NH 2 and L is 5(thioacetamido)-fluorescein.
Claim 4
Dependent Claim
13 words
The compound of claim 1 wherein X=NH 2 Y=H and L is 5(thioacetamido)-fluorescein.
Claim 5
Dependent Claim
14 words
The compound of claim 1 wherein X=OH, Y=NH 2 and L is 5(2-thioacetamido-ethyl)-amino-naphthalene-1-sulphonic acid.
Claim 6
Dependent Claim
16 words
The compound of claim 1 wherein X=NH 2 , Y=H and L is 5(2 thioacetamido-ethyl)-amino-naphthalene-1-sulphonic acid.
Claim 7
Dependent Claim
13 words
The compound of claim 1 wherein X=OH, Y=NH 2 and L is 6(thioacetamido)-fluorescein.
Claim 8
Dependent Claim
14 words
The compound of claim 1 wherein X=NH 2 , Y=H and L is 6(thioacetamido)-fluorescein.
Claim 9
Dependent Claim
14 words
The compound of claim 1 wherein X=OH Y=NH 2 and L is 8(2-thioacetamido-ethyl)-amino-naphthalene-1-sulphonic acid.
Claim 10
Dependent Claim
14 words
The compound of claim 1 wherein X=NH 2 Y=H and L is 8-(2-thioacetamido-ethyl)-amino-naphthalene-1-sulphonic acid.
Claim 11
Dependent Claim
10 words
The compound of claim 1 wherein L is 5 (thioacetamido)-2',4',5',7'-tetrabormofluorescein.
Claim 12
Dependent Claim
9 words
The compound of claim 1 wherein L is 5(thioacetamido)-2'4'5'7'-tetraiodo-fluorescein.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-01-10 | 1992-07-11 | 1993-01-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-01-10 | 1996-07-11 | 1997-01-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-01-10 | 2000-07-11 | 2001-01-10 | $7700.00 | Window Closed / Lapsed |