US4797359A
Expired / Lapsed
US
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Heat shock regulated production of selected and fused proteins in yeast
Assignee: The University of Texas System
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Filing: 1983-05-10
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Granted: 1989-01-10
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Expires: 2003-05-10
Abstract
Abstract A process for producing a yeast capable of heat regulated synthesis of selected and fused proteins is disclosed. The process involves the combination of a DNA fragment comprising a heat shock inducible gene of an appropriate yeast microorganism and a second DNA fragment comprising a selected gene with a suitable transfer vector, thereby producing a recombinant transfer vector. A suitable host yeast is contacted with the recombinant transfer vector, cultured and transforments having the desired genetic capability are selected.
Inventors
- 👤 David B. Finkelstein
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 14:16
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/37; C07K14/39; C07K14/395; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/80; C12N15/81; C12N9/00; C12N9/14; C12N9/24; C12N9/2402; C12N9/2468; C12N9/2471; C12Y; C12Y302/00; C12Y302/01; C12Y302/01023; C07K2319/00; C07K2319/35; C07K2319/61
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
153 words
A process for producing a transformed S. cerevisiae yeast microorganism capable of heat regulated synthesis of a selected unfused protein, which comprises: (1) cleaving DNA having a heat shock inducible gene of a S. cerevisiae yeast microorganism to produce a promoter sequence of said heat shock inducible gene and excluding the structural gene sequence coding for the heat shock protein; (2) preparing a recombinant transfer vector by combining a suitable transfer vector with said promoter sequence obtained in step (1) and a DNA fragment comprising a DNA sequence encoding for said selected heterologous protein, said transfer vector being capable of replication in a host yeast microorganism, said promoter sequence and said DNA fragment being capable of recombination; (3) transforming said host yeast microroganism with said recombinant transfer vector; (4) culturing said transformed host yeast microorganism; and (5) selecting a transformed host microorganism capable of heat regulated synthesis of said selected unfused heterologous protein.
Claim 2
Dependent Claim
153 words
A process for producing a transformed S. cerevisiae yeast microorganism capable of heat regulated synthesis of a selected unfused protein, which comprises: (1) cleaving DNA having a heat shock inducible gene of a S. cerevisiae yeast microorganism to produce a promoter sequence of said heat shock inducible gene and excluding the structural gene sequence coding for the heat shock protein; (2) preparing a recombinant transfer vector by combining a suitable transfer vector with said promoter sequence obtained in step (1) and a DNA fragment comprising a DNA sequence encoding for said selected heterologous protein, said transfer vector being capable of replication in a host yeast microorganism, said promoter sequence and said DNA fragment being capable of recombination; (3) transforming said host yeast microroganism with said recombinant transfer vector; (4) culturing said transformed host yeast microorganism; and (5) selecting a transformed host microorganism capable of heat regulated synthesis of said selected unfused heterologous protein.
Claim 3
Dependent Claim
13 words
The process of claim 1 wherein said heat shock inducible gene is HSP90.
Claim 4
Dependent Claim
16 words
The process of claim 1 wherein said DNA fragment comprises a lacZ gene of E. coli.
Claim 5
Dependent Claim
15 words
The process of claim 1 wherein said transfer vector is derived from a plasmid DNA.
Claim 6
Dependent Claim
11 words
The process of claim 4 wherein said plasmid DNA is pUTX37.
Claim 7
Dependent Claim
34 words
The process of claim 1, wherein said promoter sequence is further defined as a 0.201 kilobase pair Hind III - Bam HI DNA fragment from the Heat Shock promoter region of S. cerevisiae HSP90.
Claim 8
Dependent Claim
72 words
A transformed S. cerevisiae yeast microorganism, capable of heat regulated synthesis of a selected unfused heterologous protein, containing therein a plasmid or other DNA sequence capable of replication in said yeast microorganism and comprising the combination of a promoter sequence of S. cerevisiae heat shock inducible gene excluding the structural gene sequence coding for the heat shock protein, and a second DNA fragment having a DNA sequence encoding for said selected protein.
Claim 9
Dependent Claim
72 words
A transformed S. cerevisiae yeast microorganism, capable of heat regulated synthesis of a selected unfused heterologous protein, containing therein a plasmid or other DNA sequence capable of replication in said yeast microorganism and comprising the combination of a promoter sequence of S. cerevisiae heat shock inducible gene excluding the structural gene sequence coding for the heat shock protein, and a second DNA fragment having a DNA sequence encoding for said selected protein.
Claim 10
Dependent Claim
14 words
The transformed microorganism of claim 7 wherein said heat shock iducible gene is HSP90.
Claim 11
Dependent Claim
18 words
The transformed microroganism of claim 7 wherein said second DNA fragment comprises a lacZ gene of E. coli.
Claim 12
Dependent Claim
36 words
The transfromed yeast microorganism of claim 7, wherein said promoter sequence is further defined as a 0.201 kilobase pair Hind III - Bam HI DNA fragment from the Heat Shock promoter region of S. cerevisiase HSP90.
Claim 13
Dependent Claim
75 words
A process for heat regulated synthesis of a selected unfused heterologous protein within a transformed S. cerevisiae yeast microroganism, which comprises culturing said yeast microroganism, said microorganism having been transformed with a recombinant transfer vector comprising the combination of a prmomoter sequence of a S. cerevisiae heat shock inducible gene excluding the structral gene sequence coding for the heat shock protein, and a second DNA fragment having a DNA sequence encoding for said selected protein.
Claim 14
Dependent Claim
75 words
A process for heat regulated synthesis of a selected unfused heterologous protein within a transformed S. cerevisiae yeast microroganism, which comprises culturing said yeast microroganism, said microorganism having been transformed with a recombinant transfer vector comprising the combination of a prmomoter sequence of a S. cerevisiae heat shock inducible gene excluding the structral gene sequence coding for the heat shock protein, and a second DNA fragment having a DNA sequence encoding for said selected protein.
Claim 15
Dependent Claim
13 words
The process of claim 11 wherein said heat shock inducible gene is HSP90.
Claim 16
Dependent Claim
17 words
The process of claim 11 wherein said second dNA fragment comprises a lacZ gene of E. coli.
Claim 17
Dependent Claim
34 words
The process of claim 11, wherein said prpomoter sequence is further defined as a 0.201 kilobase pair Hind III - Bam HI DNA fragment from the Heat Shock promoter region of S. cerevisiae HSP90.
Claim 18
Dependent Claim
6 words
The transformed microorganism S. cerevisiae DC5/pUTX41.
Claim 19
Dependent Claim
6 words
The transformed microorganism S. cerevisiae DC5/pUTX41.
References Cited (Backward Citations)
US4374927A
Cited
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| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1992-01-10 | 1992-07-11 | 1993-01-10 | $2000.00 | Window Closed / Lapsed |
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