US4793996A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method of making soybean extract inhibitor
Assignee: TRUSTEES OF UNIVERSITY OF PENNSYLVANIA A NON-PROFIT Corp OF PENNSYLVANIA
•
Filing: 1986-09-25
•
Granted: 1988-12-27
•
Expires: 2006-09-25
Abstract
Abstract In the process of preparing a soybean extract exhibiting inhibition of malignant transformation of cells which comprises defatting soybeans and extracting the inhibitor from the defatted soybeans, the step of defatting the soybeans by bringing them into contact with acetone provides an edible extract of increased effectiveness.
Inventors
- 👤 Ann R. Kennedy
- 👤 Walter Troll
- 👤 Jonathan Yavelow
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 23:00
CPC Classifications
A; A61; A61K; A61K36/00; A61K36/18; A61K36/185; A61K36/48
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
125 words
A process for preparing a Bowman-Birk inhibitor from soybeans which comprises the steps of: (a) treating soybeans with at least an equal weight of acetone by mixing said soybeans in a dry, finely-divided form with said acetone for a sufficient time and at a sufficient temperature to provide said inhibitor with the capability of inhibiting malignant transformation of cells; (b) extracting the treated soybeans with ethyl alcohol or aqueous ethyl alcohol for a sufficient time and at a sufficient temperature to form a solution containing said inhibitor; (c) separating residual solids from said solution; and (d) precipitating the inhibitor from said solution by mixing the solution with acetone, whereby an inhibitor is obtained which is capable of inhibiting malignant transformation of C3H mouse embryo cells.
Claim 2
Dependent Claim
125 words
A process for preparing a Bowman-Birk inhibitor from soybeans which comprises the steps of: (a) treating soybeans with at least an equal weight of acetone by mixing said soybeans in a dry, finely-divided form with said acetone for a sufficient time and at a sufficient temperature to provide said inhibitor with the capability of inhibiting malignant transformation of cells; (b) extracting the treated soybeans with ethyl alcohol or aqueous ethyl alcohol for a sufficient time and at a sufficient temperature to form a solution containing said inhibitor; (c) separating residual solids from said solution; and (d) precipitating the inhibitor from said solution by mixing the solution with acetone, whereby an inhibitor is obtained which is capable of inhibiting malignant transformation of C3H mouse embryo cells.
Claim 3
Dependent Claim
23 words
The process of claim 1 wherein step (a) is carried out at a temperature from room temperature to the boiling point of acetone.
Claim 4
Dependent Claim
27 words
The process of claim 2 wherein the weight of acetone in step (a) is from about five times to about ten times the weight of the soybeans.
Claim 5
Dependent Claim
12 words
The process of claim 3 which further comprises purifying the acetone-precipitated inhibitor.
Claim 6
Dependent Claim
20 words
The process of claim 4 wherein said soybeans have been defatted prior to the treatment with acetone in step (a).
Claim 7
Dependent Claim
20 words
The process of claim 3 wherein said soybeans have been defatted prior to the treatment with acetone in step (a).
Claim 8
Dependent Claim
12 words
The process of claim 2 which further comprises purifying the acetone-precipitated inhibitor.
Claim 9
Dependent Claim
20 words
The process of claim 7 wherein said soybeans have been defatted prior to the treatment with acetone in step (a).
Claim 10
Dependent Claim
20 words
The process of claim 2 wherein said soybeans have been defatted prior to the treatment with acetone in step (a).
Claim 11
Dependent Claim
27 words
The process of claim 1 wherein the weight of acetone in step (a) is from about five times to about ten times the weight of the soybeans.
Claim 12
Dependent Claim
12 words
The process of claim 10 which further comprises purifying the acetone-precipitated inhibitor.
Claim 13
Dependent Claim
20 words
The process of claim 11 wherein said soybeans have been defatted prior to the treatment with acetone in step (a).
Claim 14
Dependent Claim
20 words
The process of claim 10 wherein said soybeans have been defatted prior to the treatment with acetone in step (a).
Claim 15
Dependent Claim
12 words
The process of claim 1 which further comprises purifying the acetone-precipitated inhibitor.
Claim 16
Dependent Claim
20 words
The process of claim 14 wherein said soybeans have been defatted prior to the treatment with acetone in step (a).
Claim 17
Dependent Claim
20 words
The process of claim 1 wherein said soybeans have been defatted prior to the treatment with acetone in step (a).
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-12-27 | 1992-06-27 | 1992-12-27 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-12-27 | 1996-06-27 | 1996-12-27 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-12-27 | 2000-06-27 | 2000-12-27 | $7700.00 | Window Closed / Lapsed |