US4792525A
Expired / Lapsed
US
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Tetrapeptide
Assignee: Sanford Burnham Prebys Medical Discovery Institute
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Filing: 1985-06-17
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Granted: 1988-12-20
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Expires: 2005-06-17
Abstract
Abstract The peptide X-Arg-Gly-Asp-R-Y wherein X is H or at least one amino acid and Y is OH or at least one amino acid, and R is an amino acid selected from Thr or Cys, or other amino acid, having the same cell-attachent activity as fibronectin and the peptide X-Arg-Gly-Asp-Ser-Y, wherein X and Y, having said activity are disclosed.
Inventors
- 👤 Erkki Ruoslahti
- 👤 Michael Pierschbacher
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 21:34
CPC Classifications
A; A61; A61K; A61K38/00; A61K38/04; A61K38/07; A61K38/08; A61L; A61L27/00; A61L27/14; A61L27/22; A61L27/227; C; C07; C07K; C07K14/00; C07K14/435; C07K14/78; Y; Y10; Y10S; Y10S530/00; Y10S530/81; Y10S530/811; Y10S530/812; Y10S530/815
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
56 words
A method for promoting cell attachment to a substrate comprising the steps of: immobilizing on the substrate a non-naturally occurring polypeptide which includes as the cell-attachment-promoting constituent the sequence -Arg-Gly-Asp-R- wherein R is Ser, Cys, Thr or other amino acid such that the polypeptide has cell-attachment-promoting activity; and providing free cells for attachment to said substrate,.
Claim 2
Independent Claim
56 words
A method for promoting cell attachment to a substrate comprising the steps of: immobilizing on the substrate a non-naturally occurring polypeptide which includes as the cell-attachment-promoting constituent the sequence -Arg-Gly-Asp-R- wherein R is Ser, Cys, Thr or other amino acid such that the polypeptide has cell-attachment-promoting activity; and providing free cells for attachment to said substrate,.
Claim 3
Independent Claim
78 words
A method for promoting cell attachment to a substrate comprising the steps of: immobilizing on the substrate a composition of matter comprising a non-naturally occurring peptide X-Arg-Gly-Asp-R-Y wherein X is H or at least one amino acid and Y is OH or at least one amino acid, and R is an amino acid selected from Ser Thr, Cys or other amino acid, such that said peptide has cell-attachmentpromoting activity; and providing free cells for attachment to the substrate.
Claim 4
Independent Claim
78 words
A method for promoting cell attachment to a substrate comprising the steps of: immobilizing on the substrate a composition of matter comprising a non-naturally occurring peptide X-Arg-Gly-Asp-R-Y wherein X is H or at least one amino acid and Y is OH or at least one amino acid, and R is an amino acid selected from Ser Thr, Cys or other amino acid, such that said peptide has cell-attachmentpromoting activity; and providing free cells for attachment to the substrate.
Claim 5
Independent Claim
32 words
A method of promoting cell attachment activity comprising the steps of: immobilizing on a substrate a non-naturally occurring polypeptide including the sequence Arg-Gly-Asp-Ser; and providing free cells for attachment to said substrate.
Claim 6
Independent Claim
32 words
A method of promoting cell attachment activity comprising the steps of: immobilizing on a substrate a non-naturally occurring polypeptide including the sequence Arg-Gly-Asp-Ser; and providing free cells for attachment to said substrate.
Claim 7
Independent Claim
64 words
A method for promoting cell attachment to a substrate comprising the steps of: immobilizing on the substrate a substantially pure peptide including as the cell-attachment-promoting constituent the amino acid sequence Arg-Gly-Asp-R wherein R is Ser, Cys, Thr or other amino acid, said peptide having cell-attachment-promoting activity, and said peptide not being a naturally occurring peptide; and providing free cells for attachment to said substrate.
Claim 8
Independent Claim
64 words
A method for promoting cell attachment to a substrate comprising the steps of: immobilizing on the substrate a substantially pure peptide including as the cell-attachment-promoting constituent the amino acid sequence Arg-Gly-Asp-R wherein R is Ser, Cys, Thr or other amino acid, said peptide having cell-attachment-promoting activity, and said peptide not being a naturally occurring peptide; and providing free cells for attachment to said substrate.
Claim 9
Independent Claim
68 words
A method of promoting cell attachment to a substrate, comprising the steps of: immobilizing on the substrate a polypeptide which includes as the cell-attachment-promoting constituent the amino acid sequence Arg-Gly-Asp-R wherein R is Ser, Cys, Thr or other amino acid such that the polypeptide has cell attachment promoting activity, said polypeptide having less than about 31 amino acid residues; and providing free cells for attachment to said substrate.
Claim 10
Independent Claim
68 words
A method of promoting cell attachment to a substrate, comprising the steps of: immobilizing on the substrate a polypeptide which includes as the cell-attachment-promoting constituent the amino acid sequence Arg-Gly-Asp-R wherein R is Ser, Cys, Thr or other amino acid such that the polypeptide has cell attachment promoting activity, said polypeptide having less than about 31 amino acid residues; and providing free cells for attachment to said substrate.
Claim 11
Independent Claim
87 words
A method of prommoting cell attachment to a substrate, comprising the steps of: immobilizing on the substrate a composition of matter comprising a substantially pure polypeptide X-Arg-Gly-Asp-R-Y wherein X is H or at least one amino acid and Y is OH or at least one amino acid, and R is an amino acid selected from Ser, Syc, Thr or other amino acid, said peptide having cell attachment promoting activity, and said peptide not being a naturally occurring peptide; and providing free cells for attachment to said substrate.
Claim 12
Independent Claim
87 words
A method of prommoting cell attachment to a substrate, comprising the steps of: immobilizing on the substrate a composition of matter comprising a substantially pure polypeptide X-Arg-Gly-Asp-R-Y wherein X is H or at least one amino acid and Y is OH or at least one amino acid, and R is an amino acid selected from Ser, Syc, Thr or other amino acid, said peptide having cell attachment promoting activity, and said peptide not being a naturally occurring peptide; and providing free cells for attachment to said substrate.
Claim 13
Independent Claim
88 words
A method of promoting cell attachment to a substrate, comprising the steps of: immobilizing on the substrate a composition of matter comprising a polypeptide X-Arg-Gly-Asp-R-Y wherein X is H or at least one amino acid and Y is OH or at least one amino acid, and R is an amino acid selected from Ser, Cys, Thr or other amino acid such that said peptide has cell attachment promoting activity, said polypeptide having less than about 31 amino acid residues; and providing free cells for attachment to said substrate.
Claim 14
Independent Claim
88 words
A method of promoting cell attachment to a substrate, comprising the steps of: immobilizing on the substrate a composition of matter comprising a polypeptide X-Arg-Gly-Asp-R-Y wherein X is H or at least one amino acid and Y is OH or at least one amino acid, and R is an amino acid selected from Ser, Cys, Thr or other amino acid such that said peptide has cell attachment promoting activity, said polypeptide having less than about 31 amino acid residues; and providing free cells for attachment to said substrate.
Claim 15
Dependent Claim
38 words
A substantially pure peptide including as the cell-attachment-promoting constituent the amino acid sequence Arg-Gly-Arg-R wherein R is Ser, Cys, Thr or other amino acid, said peptide having cell-attachmentpromoting activity, and said peptide not being a naturally occurring peptide.
Claim 16
Dependent Claim
38 words
A substantially pure peptide including as the cell-attachment-promoting constituent the amino acid sequence Arg-Gly-Arg-R wherein R is Ser, Cys, Thr or other amino acid, said peptide having cell-attachmentpromoting activity, and said peptide not being a naturally occurring peptide.
Claim 17
Dependent Claim
14 words
A composition of matter comprising the peptide of claim 8 attached to a substrate.
Claim 18
Dependent Claim
40 words
A peptide including as the cell-attachment-promoting constituent the amino acid sequence Arg-Gly-Asp-R, wherein R is Ser, Cys, Thr or other amino acid such that the peptide has cell-attachment-promoting activity, wherein said peptide has less than about 31 amino acid residues.
Claim 19
Dependent Claim
40 words
A peptide including as the cell-attachment-promoting constituent the amino acid sequence Arg-Gly-Asp-R, wherein R is Ser, Cys, Thr or other amino acid such that the peptide has cell-attachment-promoting activity, wherein said peptide has less than about 31 amino acid residues.
Claim 20
Dependent Claim
14 words
A composition of matter comprising the peptide of claim 10 attached to a substrate.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1991-12-20 | 1992-06-20 | 1992-12-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-12-20 | 1996-06-20 | 1996-12-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-12-20 | 2000-06-20 | 2000-12-20 | $7700.00 | Window Closed / Lapsed |