US4791069A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Methods for attaching ligands or anti-ligands to a solid phase
Assignee: Ortho Clinical Diagnostics Inc
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Filing: 1987-05-08
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Granted: 1988-12-13
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Expires: 2007-05-08
Abstract
Abstract Methods for attaching one component of a ligand-anti-ligand pair onto a solid phase surface. The component may be attached to a photoactivatable cross-linker capable of coupling the component to a colloidal medium coating the surface or alternatively, the component may be coupled to a bead and held in place against the surface by an overlay of the colloidal media having voids and spaces smaller than the diameter of the particle but large enough to permit diffusion of the other of the ligand-anti-ligand pair to be detected.
Inventors
- 👤 George Hovorka
- 👤 W. Peter Hansen
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 01:04
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; Y; Y10; Y10S; Y10S436/00; Y10S436/807; Y10S436/809
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
150 words
A method for preparing a solid phase component useful in an immunoassay based on pattern detection, wherein a first member of a ligand-anti-ligand pair present in an aqueous sample is detected by reaction with a second member of the pair present on said insoluble surface in a spatial pattern, the method comprising the steps of: (a) providing said insoluble surface; (b) providing beads having attached thereto said second member; (c) placing said beads on said surface in a resolved spatial pattern of repeating and alternating presence and absence of immunological reactivity; and (d) coating said beads and said surface with a gel, said gel having voids and channels which allow said first member to diffuse through the gel but not said beads, whereby said beads are maintained on said surface and said first component in said sample can diffuse through the gel to said second component and react immunologically therewith.
Claim 2
Independent Claim
150 words
A method for preparing a solid phase component useful in an immunoassay based on pattern detection, wherein a first member of a ligand-anti-ligand pair present in an aqueous sample is detected by reaction with a second member of the pair present on said insoluble surface in a spatial pattern, the method comprising the steps of: (a) providing said insoluble surface; (b) providing beads having attached thereto said second member; (c) placing said beads on said surface in a resolved spatial pattern of repeating and alternating presence and absence of immunological reactivity; and (d) coating said beads and said surface with a gel, said gel having voids and channels which allow said first member to diffuse through the gel but not said beads, whereby said beads are maintained on said surface and said first component in said sample can diffuse through the gel to said second component and react immunologically therewith.
Claim 3
Independent Claim
146 words
A method for preparing a solid phase component useful in an immunoassay based on pattern detection, wherein a first member of a ligand-anti-ligand pair present in an aqueous sample is detected by reaction with a second member of the pair present on an insoluble surface in a spatial pattern, the method comprising the steps of: (a) providing said insoluble surface; (b) providing beads having attached thereto said second member; (c) coating said surface with a gel, said gel having voids and channels which allow said first member to diffuse through the gel, but not said beads; (d) placing said beads in said gel in a resolved spatial pattern of repeating and alternating presence and absence of immunological reactivity, whereby said beads are maintained on said surface and said first component in said sample can diffuse through the gel to said second component and react immunologically therewith.
Claim 4
Independent Claim
146 words
A method for preparing a solid phase component useful in an immunoassay based on pattern detection, wherein a first member of a ligand-anti-ligand pair present in an aqueous sample is detected by reaction with a second member of the pair present on an insoluble surface in a spatial pattern, the method comprising the steps of: (a) providing said insoluble surface; (b) providing beads having attached thereto said second member; (c) coating said surface with a gel, said gel having voids and channels which allow said first member to diffuse through the gel, but not said beads; (d) placing said beads in said gel in a resolved spatial pattern of repeating and alternating presence and absence of immunological reactivity, whereby said beads are maintained on said surface and said first component in said sample can diffuse through the gel to said second component and react immunologically therewith.
Claim 5
Dependent Claim
15 words
The method of claim 1, wherein the beads are placed by painting, spraying or printing.
Claim 6
Dependent Claim
17 words
The method of claim 3, wherein the beads are placed by ink jet printing, airbrushing or silk-screening.
Claim 7
Dependent Claim
19 words
The method of claim 1, wherein the ligand is an antigen and the anti-ligand is an antibody specific therefor.
Claim 8
Dependent Claim
12 words
The method of claim 1, wherein the anti-ligand is an antibody fragment.
Claim 9
Dependent Claim
15 words
The method of claim 6, wherein the antibody fragment is an F(b) or F(ab)'2 fragment.
Claim 10
Dependent Claim
16 words
The method of claim 1, wherein the insoluble surface is a plastic or glass flat surface.
Claim 11
Dependent Claim
12 words
The method of claim 8, wherein the surface is a microscope slide.
Claim 12
Dependent Claim
17 words
The method of claim 1, wherein the insoluble surface is the wall of a microtiter tray well.
Claim 13
Dependent Claim
12 words
The method of claim 1, wherein the gel is a polysaccharide gel.
Claim 14
Dependent Claim
10 words
The method of claim 11, wherein the gel is agarose.
Claim 15
Dependent Claim
14 words
The method of claim 1, wherein said gel is about 50 microns in thickness.
Claim 16
Dependent Claim
18 words
The method of claim 1, wherein the beads are constructed of a polymer of synthetic or natural origin.
Claim 17
Dependent Claim
12 words
The method of claim 14, wherein the beads are microspheres or macrospheres.
Claim 18
Dependent Claim
11 words
The method of claim 1, wherein the beads are glass microspheres.
Claim 19
Dependent Claim
15 words
The method of claim 1, wherein the second member is covalently attached to said beads.
Claim 20
Dependent Claim
30 words
The method of claim 2, wherein before step (d) the beads are mixed into a solution of said gel and then are placed in said gel as in step (d).
References Cited (Backward Citations)
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