US4785079A
Expired / Lapsed
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Isolation of fibroblast growth factor
Assignee: Salk Institute for Biological Studies
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Filing: 1986-12-10
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Granted: 1988-11-15
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Expires: 2006-12-10
Abstract
Abstract Basic Fibroblast Growth Factor (FGF) is substantially purified by the employment of affinity chromatography using heparin-linked support material. Described is a simplified three step procedure for extracting basic FGF from either mammalian brain or mammalian pituitary tissue. Salt precipitation, e.g., with ammonium sulfate is used to provide a partially purified precipitate that is then subjected to ion-exchange chromatography, e.g., using a Carboxymethyl-Sephadex column. Substantially pure basic FGF fractions are then obtained by fractionating the further partially purified fractions using affinity chromatography on a heparin-linked support e.g., Heparin-Sepharose.
Inventors
- 👤 Denis Gospodarowicz
- 👤 Andrew Baird
- 👤 Peter Bohlen
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 13:18
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/475; C07K14/50; C07K14/503
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
103 words
A method of obtaining substantially pure basic fibroblast growth factor comprising providing a biological sample containing significant levels of basic fibroblast growth factor, extracting the solubilized component of said biological sample, partially purifying basic FGF from said extract by a salt-precipitation step, then fractionating said partially-purified basic FGF-containing material so as to remove neutral and acidic proteins, and then treating said fractionated basic FGF-containing material using affinity chromatography employing heparin moieties linked to an insoluble support as the stationary phase and employing a salt gradient mobile phase of increasing salt concentration, and collecting liquid fractions containing substantially purified basic fibroblast growth factor therefrom.
Claim 2
Independent Claim
103 words
A method of obtaining substantially pure basic fibroblast growth factor comprising providing a biological sample containing significant levels of basic fibroblast growth factor, extracting the solubilized component of said biological sample, partially purifying basic FGF from said extract by a salt-precipitation step, then fractionating said partially-purified basic FGF-containing material so as to remove neutral and acidic proteins, and then treating said fractionated basic FGF-containing material using affinity chromatography employing heparin moieties linked to an insoluble support as the stationary phase and employing a salt gradient mobile phase of increasing salt concentration, and collecting liquid fractions containing substantially purified basic fibroblast growth factor therefrom.
Claim 3
Dependent Claim
59 words
A method according to claim 1 wherein said fractionating is carried out using ion-exchange chromatography with a stationary phase selected from the group consisting of cross-linked dextran having carboxymethyl functional moieties, cross-linked dextran having sulfopropyl functional moieties and polystyrene cross-linked with divinylbenzene having sulfate functional moieties, and wherein said basic fibroblast growth factor is adsorbed onto said stationary phase.
Claim 4
Dependent Claim
21 words
A method according to claim 1 wherein said biological sample is homogenized and the soluble extract is treated with ammonium sulfate.
Claim 5
Dependent Claim
28 words
A method according to claim 3 wherein said homogenized biological sample is adjusted to have a pH of about 4.5 at the time of treatment with ammonium sulfate.
Claim 6
Dependent Claim
29 words
A method according to claim 1 wherein said mobile phase has a generally neutral pH and has a NaCl concentration ranging from about 0.6 M to about 2.0 M.
Claim 7
Dependent Claim
29 words
A method according to claim 1 wherein said partially purified fibroblast growth factor fractionated material contains basic FGF in an amount of at least about 0.05% of total protein.
Claim 8
Dependent Claim
26 words
A method according to claim 1 wherein heparin-linked affinity chromatography produces fractions which contain greater than 90% basic fibroblast growth factor by weight of total protein.
Claim 9
Dependent Claim
19 words
A method according to claim 1 wherein said biological sample is either mammalian brain tissue or mammalian pituitary tissue.
Claim 10
Dependent Claim
18 words
A method according to claim 8 wherein said material is either bovine brain tissue or bovine pituitary tissue.
Claim 11
Independent Claim
65 words
A method of obtaining substantially pure basic fibroblast growth factor comprising providing material containing significant levels of basic fibroblast growth factor, and treating fractionated basic FGF-containing material using affinity chromatography employing heparin moieties bound to an insoluble support as the stationary phase and employing a salt gradient mobile phase of increasing salt concentration, and collecting liquid fractions containing substantially purified basic fibroblast growth factor therefrom.
Claim 12
Independent Claim
65 words
A method of obtaining substantially pure basic fibroblast growth factor comprising providing material containing significant levels of basic fibroblast growth factor, and treating fractionated basic FGF-containing material using affinity chromatography employing heparin moieties bound to an insoluble support as the stationary phase and employing a salt gradient mobile phase of increasing salt concentration, and collecting liquid fractions containing substantially purified basic fibroblast growth factor therefrom.
Claim 13
Dependent Claim
23 words
A method according to claim 10 wherein said material contains basic FGF in an amount of at least about 0.05% of total protein.
Claim 14
Dependent Claim
27 words
A method according to claim 11 wherein said heparin-linked affinity chromatography produces fractions which contain greater than 90% basic fibroblast growth factor by weight of total protein.
Claim 15
Independent Claim
83 words
A method of obtaining substantially pure basic fibroblast growth factor from an impure mixture containing significant levels of basic fibroblast growth factor, which method comprises applying said mixture to an affinity chromatography column comprising heparin moieties bound to a particulate support as the stationary phase, and eluting said basic FGF from said support with an aqueous eluant mobile phase of generally neutral pH and a salt concentration which increases and is above 0.6 M and collecting substantially purified basic fibroblast growth factor therefrom.
Claim 16
Independent Claim
83 words
A method of obtaining substantially pure basic fibroblast growth factor from an impure mixture containing significant levels of basic fibroblast growth factor, which method comprises applying said mixture to an affinity chromatography column comprising heparin moieties bound to a particulate support as the stationary phase, and eluting said basic FGF from said support with an aqueous eluant mobile phase of generally neutral pH and a salt concentration which increases and is above 0.6 M and collecting substantially purified basic fibroblast growth factor therefrom.
References Cited (Backward Citations)
US4444760A
Cited
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