US4783419A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Examining cells by electrophoresis
Assignee: Kureha Corp
•
Filing: 1985-03-21
•
Granted: 1988-11-08
•
Expires: 2005-03-21
Abstract
Abstract Disclosed herein is a method for examining cells, comprising subjecting the cells to an antigen-antibody reaction treatment, then measuring a pattern of the electrophoretic mobility of the cells and comparing the electrophoretic property of the cells under examination with the electrophoretic property of standard cells.
Inventors
- 👤 Haruhisa Hayashi
- 👤 Yoshiharu Oguchi
- 👤 Kenichi Matsunaga
- 👤 Chikao Yoshikumi
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 13:29
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; Y; Y10; Y10S; Y10S436/00; Y10S436/806; Y10S436/811; Y10S436/813
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
58 words
A method for examining cells, comprising: subjecting specimen cells to an antigen-antibody reaction treatment; measuring a pattern of the electrophoretic mobility of the treated specimen cells in a physiological medium having an ionic strength of 0.11 to 0.21 moles/l; and comparing the electrophoretic mobility of the treated specimen cells under examination with the electrophoretic mobility of control cells.
Claim 2
Independent Claim
58 words
A method for examining cells, comprising: subjecting specimen cells to an antigen-antibody reaction treatment; measuring a pattern of the electrophoretic mobility of the treated specimen cells in a physiological medium having an ionic strength of 0.11 to 0.21 moles/l; and comparing the electrophoretic mobility of the treated specimen cells under examination with the electrophoretic mobility of control cells.
Claim 3
Dependent Claim
12 words
The method of claim 1, comprising using untreated cells as control cells.
Claim 4
Dependent Claim
22 words
The method of claim 1, comprising conducting the electrophoresis measurements with an apparatus capable of automatically measuring the electrophoretic mobility of cells.
Claim 5
Dependent Claim
28 words
The method of claim 1, comprising using a concentration of specimen cells in the range of 0.5×10 6 cell ml -1 to 20×10 6 cell ml -1 .
Claim 6
Dependent Claim
18 words
The method of claim 4, comprising using a medium having an ionic strength of 0.13 to 0.17 moles/l.
Claim 7
Dependent Claim
19 words
The method of claim 1, wherein the specimen cells are red blood cells, white blood cells, or cancer cells.
Claim 8
Dependent Claim
10 words
The method of claim 1, comprising examining lymphocytes or macrophages.
Claim 9
Dependent Claim
29 words
The method of claim 1, wherein the specimen cells are cells suspected of having been affected by a tumor, bronchial asthma, a nervous system disease, or an autoimmune disease.
Claim 10
Dependent Claim
33 words
The method of claim 1, comprising measuring the electrophoretic mobility of specimen cells to detected the difference between normal cells and cells affected with a disease to detect a change in the disease.
Claim 11
Dependent Claim
26 words
The method of claim 1, wherein the specimen cells are cells suspected of having been affected by a change in the immune status of a patient.
Claim 12
Dependent Claim
13 words
The method of claim 1, comprising reacting the specimen cells with an antibody.
Claim 13
Dependent Claim
20 words
The method of claim 11, wherein the antibody is a monoclonal antibody obtained from hybrid cells prepared by cell fusion.
Claim 14
Dependent Claim
12 words
The method of claim 11, wherein the antibody is a human antibody.
Claim 15
Dependent Claim
12 words
The method of claim 11, wherein the antibody is a polyclonal antibody.
Claim 16
Dependent Claim
22 words
The method of claim 14, wherein the polyclonal antibody is obtained from an immunized rabbit, goat, mouse, horse, sheep, chicken, or ape.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-11-08 | 1992-05-09 | 1992-11-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-11-08 | 1996-05-09 | 1996-11-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-11-08 | 2000-05-09 | 2000-11-08 | $7700.00 | Window Closed / Lapsed |