US4783401A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Viable cell labelling
Assignee: SKB AND ZYNAXIS CELL SCIENCE Inc A CORP OF PENNSYLVANIA
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Filing: 1986-10-31
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Granted: 1988-11-08
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Expires: 2006-10-31
Abstract
Abstract Methods for reproducibly labelling viable cells with cyanine dyes that do not significantly affect cell viability. Applications for labelled cells include using labelled red blood cells to distinguish post-transfusional bleeding from immunologic reaction and using dilution to measure growth rate of cultured cells.
Inventors
- 👤 Paul K. Horan
- 👤 Bruce D. Jensen
- 👤 Sue E. Slezak
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 14:13
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/5005; G01N33/80
Claims Analysis
Total Claims: 28
Claim 1
Independent Claim
84 words
A method for labelling viable cells with a cyanine dye that comprises contacting the cell with a cyanine dye of the structure: ##STR2## in which: Y is oxygen, sulfur, methylene, or alkylsubstituted methylene; m is 0-3; and n is 12-22 in medium containing an osmolarity regulating agent that does not significantly affect cell viability and that provides for reproducible cell labelling, said agent being selected from the group consisting of a sugar, a sugar-alcohol, an amino acid, a Good's buffer and a combination thereof.
Claim 2
Independent Claim
84 words
A method for labelling viable cells with a cyanine dye that comprises contacting the cell with a cyanine dye of the structure: ##STR2## in which: Y is oxygen, sulfur, methylene, or alkylsubstituted methylene; m is 0-3; and n is 12-22 in medium containing an osmolarity regulating agent that does not significantly affect cell viability and that provides for reproducible cell labelling, said agent being selected from the group consisting of a sugar, a sugar-alcohol, an amino acid, a Good's buffer and a combination thereof.
Claim 3
Dependent Claim
10 words
The method of claim 2 wherein the medium is iso-osmotic.
Claim 4
Dependent Claim
13 words
The method of claim 2 wherein the cell is a red blood cell.
Claim 5
Dependent Claim
12 words
The method of claim 2 wherein the cell is white blood cell.
Claim 6
Dependent Claim
11 words
The method of claim 2 wherein the cell is a platelet.
Claim 7
Dependent Claim
13 words
The method of claim 2 wherein the osmolarity regulating agent is a sugar.
Claim 8
Dependent Claim
10 words
The method of claim 6 wherein the sugar is glucose.
Claim 9
Dependent Claim
13 words
The method of claim 2 wherein the osmolarity regulating agent is a sugar-alcohol.
Claim 10
Dependent Claim
10 words
The method of claim 8 wherein the sugar-alcohol is mannitol.
Claim 11
Dependent Claim
14 words
The method of claim 2 wherein the osmolarity regulating agent is an amino acid.
Claim 12
Dependent Claim
11 words
The method of claim 10 wherein the amino acid is glycine.
Claim 13
Dependent Claim
14 words
The metnod of claim 2 wherein the osmolarity regulating agent is a Good's buffer.
Claim 14
Dependent Claim
11 words
The method of claim 12 wherein the Good's buffer is HEPPSO.
Claim 15
Dependent Claim
11 words
The method of claim 12 wherein the Good's buffer is EPPS.
Claim 16
Dependent Claim
11 words
The method of claim 12 wherein the Good's buffer is TAPS.
Claim 17
Dependent Claim
11 words
The method of claim 12 wherein the Good's buffer is CAPS.
Claim 18
Dependent Claim
11 words
The method of claim 12 wherein the Good's buffer is DIPSO.
Claim 19
Dependent Claim
11 words
The method of claim 2 wherein the cyanine dye is DiSC14(5).
Claim 20
Dependent Claim
11 words
The method of claim 2 wherein the cyanine dye is DiOC14(3).
Claim 21
Dependent Claim
65 words
A composition of matter comprising a cyanine dye of the structure: ##STR3## in which Y is oxygen, sulfur, methylene, or alkylsubstituted methylene; m is 0--3; and n is 12-22 dissolved in an osmolarity regulating agent that does not significantly affect cell viability, said agent being selected from the group consisting of a sugar, a sugar-alcohol, an amino acid, a Good's buffer and a combination thereof.
Claim 22
Dependent Claim
65 words
A composition of matter comprising a cyanine dye of the structure: ##STR3## in which Y is oxygen, sulfur, methylene, or alkylsubstituted methylene; m is 0--3; and n is 12-22 dissolved in an osmolarity regulating agent that does not significantly affect cell viability, said agent being selected from the group consisting of a sugar, a sugar-alcohol, an amino acid, a Good's buffer and a combination thereof.
Claim 23
Dependent Claim
12 words
A composition of matter of claim 20 wherein the sugar is glucose.
Claim 24
Dependent Claim
12 words
A composition of matter of claim 21 wherein the sugar-alcohol is mannitol.
Claim 25
Dependent Claim
13 words
A composition of matter of claim 21 wherein the amino acid is glycine.
Claim 26
Dependent Claim
18 words
A composition of matter of claim 20 wherein the Good's buffer is HEPPSO, EPPS, TAPS, CAPS, or DIPSO.
Claim 27
Dependent Claim
19 words
A composition of matter of claim 20 wherein the cyanine dye is DISC 14 (5) or DIOC 14 (3).
Claim 28
Dependent Claim
13 words
The method of claim 2 wherein the cell is a tissue culture cell.
References Cited (Backward Citations)
US4343782A
Cited
US4424201A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-11-08 | 1992-05-09 | 1992-11-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-11-08 | 1996-05-09 | 1996-11-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-11-08 | 2000-05-09 | 2000-11-08 | $7700.00 | Window Closed / Lapsed |