US4782025A
Expired / Lapsed
US
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Novel microorganism
Assignee: Kao Corp
•
Filing: 1985-07-15
•
Granted: 1988-11-01
•
Expires: 2005-07-15
Abstract
Abstract A novel microorganism Torulopsis bombicola KSM-36 (FERM BP-799) isolated from cabbage leaves grown in a cabbage field located in Musashino-shi, Tokyo, has sophorose-producing activity.
Inventors
- 👤 Shigeo Inoue
- 👤 Yoshiharu Kimura
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 00:48
CPC Classifications
C; C12; C12N; C12N1/00; C12N1/14; C12N1/16; C12N1/165; C12P; C12P19/00; C12P19/12; C12P7/00; C12P7/02; C12P7/04; C12P7/18; C12R; C12R2001/00; C12R2001/645; C12R2001/88; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/911; Y10S435/944
Claims Analysis
Total Claims: 2
Claim 1
Independent Claim
271 words
A biologically pure culture of Torulopsis bombicola KSM-36 (FRI Deposition FERM BP-799) having the following mycological properties: (a) state of growth on various culture media: grows into elliptical to elongated elliptical Yeasts having a size of (0.75 to 3.0)×(1.5 to 5.5) μm on malt culture medium, malt agar, potato dextrose agar, and corn meal agar, and no formation of euhyphae or pseudohyphae is observed by the multipolar budding type; (b) formation of ascospores: using malt extract agar, no formation of ascosporres is observed; (c) formation of ballistosxpores: culturing on malt agar, no formation of ballistospores is observed; ______________________________________
(d) various physiological properties:
(1) growth conditions:
pH: 3 to 8 (optimum pH: 5 to 6)
temperature: 7 to 38° C.
(optimum temperature: 25 to 34° C.)
(2) assimilation of salts (-)
of nitric acid:
(3) decomposition of fat: (+)
(4) liquefaction of gelatin:
(-)
(6) resistance to sodium chloride:
6-8% (w/v)
(7) formation of carotenoid pigments:
(-)
(8) formation of organic acids in
(+)
Caster's culture medium:
(9) formation of starch-like
(-)
substances:
(10) vitamin requirement: (+)
(e) assimilating capacity and fermenting capacity:
assimilating
fermenting
capacity capacity
(1) D-glucose (+) (+)
(2) trehalose (-) (-)
(3) D-galactose (-) (-)
(4) lactose (-) (-)
(5) sucrose (+) (+)
(6) raffinose (-) (+.sup.1/3)
(7) D-mannose (-) (-)
(8) D-xylose (-)
(9) α-methyl-D-glucoside
(-)
(10) melibiose (-)
(11) D-arabinose (-)
(12) esculin (-)
(13) ethanol (+)
(14) inositol (-)
(15) maltose (-) (-)
(16) melezitose (-)
(17) glycerin (+)
(18) cellobiose (-)
(19) inulin (-)
(20) D-mannitol (+)
(21) soluble starch (-)
(22) D-sorbitol (+)
and,
(f) productivity of sophorose:
high sophorose producing activity.
______________________________________
Claim 2
Dependent Claim
271 words
A biologically pure culture of Torulopsis bombicola KSM-36 (FRI Deposition FERM BP-799) having the following mycological properties: (a) state of growth on various culture media: grows into elliptical to elongated elliptical Yeasts having a size of (0.75 to 3.0)×(1.5 to 5.5) μm on malt culture medium, malt agar, potato dextrose agar, and corn meal agar, and no formation of euhyphae or pseudohyphae is observed by the multipolar budding type; (b) formation of ascospores: using malt extract agar, no formation of ascosporres is observed; (c) formation of ballistosxpores: culturing on malt agar, no formation of ballistospores is observed; ______________________________________
(d) various physiological properties:
(1) growth conditions:
pH: 3 to 8 (optimum pH: 5 to 6)
temperature: 7 to 38° C.
(optimum temperature: 25 to 34° C.)
(2) assimilation of salts (-)
of nitric acid:
(3) decomposition of fat: (+)
(4) liquefaction of gelatin:
(-)
(6) resistance to sodium chloride:
6-8% (w/v)
(7) formation of carotenoid pigments:
(-)
(8) formation of organic acids in
(+)
Caster's culture medium:
(9) formation of starch-like
(-)
substances:
(10) vitamin requirement: (+)
(e) assimilating capacity and fermenting capacity:
assimilating
fermenting
capacity capacity
(1) D-glucose (+) (+)
(2) trehalose (-) (-)
(3) D-galactose (-) (-)
(4) lactose (-) (-)
(5) sucrose (+) (+)
(6) raffinose (-) (+.sup.1/3)
(7) D-mannose (-) (-)
(8) D-xylose (-)
(9) α-methyl-D-glucoside
(-)
(10) melibiose (-)
(11) D-arabinose (-)
(12) esculin (-)
(13) ethanol (+)
(14) inositol (-)
(15) maltose (-) (-)
(16) melezitose (-)
(17) glycerin (+)
(18) cellobiose (-)
(19) inulin (-)
(20) D-mannitol (+)
(21) soluble starch (-)
(22) D-sorbitol (+)
and,
(f) productivity of sophorose:
high sophorose producing activity.
______________________________________
References Cited (Backward Citations)
No citation records indexed.
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