US4780412A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Fibrinolytic enzymes produced from established non-cancerous cell lines
Assignee: PUBLIC HEALTH LABORATORY SERVICE BOARD AND UNIVERSITY COLLEGE
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Filing: 1983-03-04
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Granted: 1988-10-25
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Expires: 2003-03-04
Abstract
Abstract PCT No. PCT/GB83/00067 Sec. 371 Date Nov. 4, 1983 Sec. 102(e) Date Nov. 4, 1983 PCT Filed Mar. 4, 1983 PCT Pub. No. WO83/03101 PCT Pub. Date Sep. 15, 1983.Fibrinolytic enzymes are produced and then isolated from non-cancerous established cell lines, designated as BEB or GPK. These fibrinolytic enzymes differ from those produced by malignant cell lines.
Inventors
- 👤 Anthony Atkinson
- 👤 Asgar Electricwala
- 👤 John B. Griffiths
- 👤 Amy Latter
- 👤 Patrick A. Riley
- 👤 Peter M. Sutton
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 22:36
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/64; C12N9/6421; C12N9/6424; C12N9/6456; C12Q; C12Q1/00; C12Q1/56; A; A61; A61K; A61K2123/00; A61K38/00; G; G01; G01N; G01N2333/00; G01N2333/435; G01N2333/745; G01N2333/75
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
53 words
A method of producing an enzyme having fibrinolytic activity per se or activity as a plasminogen activator which comprises culturing cells of an established cell line of non-cancerous epithelial cells designated GPK (CNCMI-222) or an established cell line of non-cancerous epithelial cells designated BEB (CNCMI-221) and isolating an enzyme-containing fraction from the culture.
Claim 2
Independent Claim
53 words
A method of producing an enzyme having fibrinolytic activity per se or activity as a plasminogen activator which comprises culturing cells of an established cell line of non-cancerous epithelial cells designated GPK (CNCMI-222) or an established cell line of non-cancerous epithelial cells designated BEB (CNCMI-221) and isolating an enzyme-containing fraction from the culture.
Claim 3
Dependent Claim
20 words
A method according to claim 1 wherein the enzyme-containing fraction is obtained by collecting a supernatant fraction containing the enzyme.
Claim 4
Dependent Claim
20 words
A method according to claim 1 wherein the enzyme-containing fraction is obtained by extracting an enzyme-containing fraction from the cells.
Claim 5
Dependent Claim
43 words
A method according to claim 1 wherein the cells are grown on a serum-containing medium, the serum-containing medium is replaced by a serum-free medium and the enzyme is recovered from a supernatant fraction comprising said serum-free medium, or extracted directly from the cells.
References Cited (Backward Citations)
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| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1991-10-25 | 1992-04-25 | 1992-10-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-10-25 | 1996-04-25 | 1996-10-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-10-25 | 2000-04-25 | 2000-10-25 | $7700.00 | Window Closed / Lapsed |