Method of testing for neutrophil function

Claim 1

A method of testing for impaired neutrophils in animals which comprises obtaining from an animal to be tested a blood sample and preparing the sample to be without lymphocytes or obtaining a neutrophil fraction thereof, culturing said treated sample or fraction in a culturing medium to which is added a testing agent comprising as an effective component a substance having a molecular weight not less than 5,000 contained in an extract extracted from a chlorella alga by an aqueous solvent and wherein the agent activates DNA synthesis in normal neutrophils and does not activate DNA synthesis in neutrophils having a reduced function and a marker for DNA synthesis, measuring the amount of marker incorporated into the neutrophils and comparing this amount with the amount incorporated in a control which does not contain the testing agent to determine if there is a larger amount of marker in the tested sample than in the control.

Claim 2

A method of testing for impaired neutrophils in animals which comprises obtaining from an animal to be tested a blood sample and preparing the sample to be without lymphocytes or obtaining a neutrophil fraction thereof, culturing said treated sample or fraction in a culturing medium to which is added a testing agent comprising as an effective component a substance having a molecular weight not less than 5,000 contained in an extract extracted from a chlorella alga by an aqueous solvent and wherein the agent activates DNA synthesis in normal neutrophils and does not activate DNA synthesis in neutrophils having a reduced function and a marker for DNA synthesis, measuring the amount of marker incorporated into the neutrophils and comparing this amount with the amount incorporated in a control which does not contain the testing agent to determine if there is a larger amount of marker in the tested sample than in the control.

Claim 4

A method of testing for impaired neutrophils in animals which comprises obtaining from an animal to be tested a blood sample and preparing the sample to be without lymphocytes or obtaining a neutrophil fraction thereof, culturing said treated sample or fraction in a culturing medium to which is added a testing agent comprising as an effective component a substance having a molecular weight not less than 5,000 which is obtained by extraction of a chlorella alga by an aqueous solvent and purified by gelfiltration chromatography or dialysis against water and wherein the agent activates DNA synthesis in normal nuetrophils and does not activate DNA synthesis in neutrophils having a reduced function and a marker for DNA synthesis, measuring the amount of marker incorporated into the neutrophils and comparing this amount with the amount incorporated in a control which does not contain the testing agent to determine if there is a larger amount of marker in the tested sample than in the control.

Claim 5

A method of testing for impaired neutrophils in animals which comprises obtaining from an animal to be tested a blood sample and preparing the sample to be without lymphocytes or obtaining a neutrophil fraction thereof, culturing said treated sample or fraction in a culturing medium to which is added a testing agent comprising as an effective component a substance having a molecular weight not less than 5,000 which is obtained by extraction of a chlorella alga by an aqueous solvent and purified by gelfiltration chromatography or dialysis against water and wherein the agent activates DNA synthesis in normal nuetrophils and does not activate DNA synthesis in neutrophils having a reduced function and a marker for DNA synthesis, measuring the amount of marker incorporated into the neutrophils and comparing this amount with the amount incorporated in a control which does not contain the testing agent to determine if there is a larger amount of marker in the tested sample than in the control.