US4777269A
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7-Phenylacetic acid-4-alkyl-coumarinyl amides useful in fluorometric determination of the activity of hydrolases
Assignee: European Atomic Energy Community Euratom
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Filing: 1987-04-30
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Granted: 1988-10-11
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Expires: 2007-04-30
Abstract
Abstract 7-Phenylacetic acid-4-alkyl-coumarinyl amides having the formula <IMAGE> (I) wherein R is alkyl having 1 to 4, in particular 1 to 2 carbon atoms, optionally substituted by one or more halogen atoms, in particular fluorine atoms, especially methyl or trifluoromethyl, are suitable substances for the fluorometric determination of the activity of hydrolases, in particular of penicillin G-acylase, since by the enzymatic reaction they provide cleavage products which in regard to their fluorescence properties differ drastically from those of the substrate. They can be used in processes for the fluorometric determination of the activity of hydrolases, in particular of penicillin G-acylase.
Inventors
- 👤 Thomas Scheper
- 👤 Martina Weiss
- 👤 Karl Schugerl
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 09:00
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/34; C07; C07D; C07D311/00; C07D311/02; C07D311/04; C07D311/06; C07D311/08; C07D311/16; C12Q2337/00; C12Q2337/20; C12Q2337/22; C12Q2337/24; G; G01; G01N; G01N2333/00; G01N2333/90; G01N2333/914; G01N2333/948
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
32 words
A 7-phenylacetic acid-4-alkyl-coumarinyl amide represented by the formula (I): ##STR6## wherein R represents an alkyl having 1 to 4 carbon atoms, which are unsubstituted or substituted by 1 or more halogen atoms.
Claim 2
Dependent Claim
32 words
A 7-phenylacetic acid-4-alkyl-coumarinyl amide represented by the formula (I): ##STR6## wherein R represents an alkyl having 1 to 4 carbon atoms, which are unsubstituted or substituted by 1 or more halogen atoms.
Claim 3
Dependent Claim
17 words
The compounds according to claim 1, characterized in that in formula (I) R is methyl or trifluoromethyl.
Claim 4
Dependent Claim
18 words
The amide according to claim 1, wherein R represents an alkyl group having 1 to 2 carbon atoms.
Claim 5
Dependent Claim
13 words
The amide according to claim 3, wherein the halogen atoms are fluorine atoms.
Claim 6
Dependent Claim
13 words
The amide according to claim 1, wherein the halogen atoms are fluorine atoms.
Claim 7
Dependent Claim
4 words
7-Phenylacetic acid-4-methyl-coumarinyl amide (PMC).
Claim 8
Dependent Claim
4 words
7-Phenylacetic acid-4-methyl-coumarinyl amide (PMC).
Claim 9
Dependent Claim
4 words
7-Phenylacetic acid-4-trifluoromethyl-coumarinyl amide (PFC).
Claim 10
Dependent Claim
4 words
7-Phenylacetic acid-4-trifluoromethyl-coumarinyl amide (PFC).
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-10-11 | 1992-04-11 | 1992-10-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-10-11 | 1996-04-11 | 1996-10-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-10-11 | 2000-04-11 | 2000-10-11 | $7700.00 | Window Closed / Lapsed |