US4775745A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Diazonium compounds useful as components for nucleic acid probes
Assignee: Columbia University
•
Filing: 1986-12-08
•
Granted: 1988-10-04
•
Expires: 2006-12-08
Abstract
Abstract This invention relates to a diazonium compound of the formula: <IMAGE> wherein Z is selected from the group consisting of biotin, an antigen, an antibody, a photoreactive group, a fluorescent group and heavy metal-containing compounds; X is an alkylene group containing up to 18 carbon atoms in the principle chain and a total of up to 24 carbon atoms or a substituted alkylene group containing up to 18 carbon atoms in the principle chain with substituents selected from the group consisting of solubility-enhancing groups and cleavable -S-S- containing moieties; Ar is an unsubstituted or substituted aryl or heteroaryl; and Y is an anion and n is an integer from 1-3. Such compounds are useful as components for nucleic acid probes.
Inventors
- 👤 John P. Ford
- 👤 Bernard F. Erlanger
- 👤 C. William Blewett
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 22:06
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6827; C12Q1/6816; Y; Y10; Y10S; Y10S436/00; Y10S436/804; Y10S436/828
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
141 words
A diazonium salt of formula: ##STR12## wherein z is selected from the group consisting of biotin, a hapten, an antigen, an antibody, a photoreactive group, a fluorescent group selected from the group consisting of Texas Red, fluorscein, rhodamine, phycoerythrins, and ferritin; X is alkylene containing up to 18 carbon atoms in the principle chain and a total of up to 24 carbon atoms; Ar is an aryl group selected from the group consisting of phenyl and alpha-and beta-naphthyl or a heteroaryl group selected from the group consisting of pyrrole, pyridine, imidazole, furan, furfuryl, thiophene, benzimidazole, thiazole, indole, quinoline and isoquioline wherein said aryl and said heteroaryl group is unsubstituted or substituted with a group selected from the group consisting of hydroxy, mercapto, halo, nitro, methylenedioxy and trifluoromethyl; and Y is an anion and n is an integer from 1 to 3.
Claim 2
Dependent Claim
141 words
A diazonium salt of formula: ##STR12## wherein z is selected from the group consisting of biotin, a hapten, an antigen, an antibody, a photoreactive group, a fluorescent group selected from the group consisting of Texas Red, fluorscein, rhodamine, phycoerythrins, and ferritin; X is alkylene containing up to 18 carbon atoms in the principle chain and a total of up to 24 carbon atoms; Ar is an aryl group selected from the group consisting of phenyl and alpha-and beta-naphthyl or a heteroaryl group selected from the group consisting of pyrrole, pyridine, imidazole, furan, furfuryl, thiophene, benzimidazole, thiazole, indole, quinoline and isoquioline wherein said aryl and said heteroaryl group is unsubstituted or substituted with a group selected from the group consisting of hydroxy, mercapto, halo, nitro, methylenedioxy and trifluoromethyl; and Y is an anion and n is an integer from 1 to 3.
Claim 3
Dependent Claim
24 words
The diazonium compound according to claim 1, having the formula: ##STR13## wherein in X, Y, n and have the meanings as in claim 1.
Claim 4
Dependent Claim
11 words
The diazonium compound according to claim 2 having the formula: ##STR14##
Claim 5
Dependent Claim
11 words
The diazonium compound according to claim 3 having the formula: ##STR15##
References Cited (Backward Citations)
US2744116A
Cited
US3637672A
Cited
US3715872A
Cited
US4539148A
Cited
US4556513A
Cited
US4617621A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-10-04 | 1992-04-04 | 1992-10-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-10-04 | 1996-04-04 | 1996-10-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-10-04 | 2000-04-04 | 2000-10-04 | $7700.00 | Window Closed / Lapsed |