US4775636A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Blot overlay assay using colloidal metal particles
Assignee: Janssen Pharmaceutica NV
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Filing: 1987-10-27
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Granted: 1988-10-04
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Expires: 2007-10-27
Abstract
Abstract A method for detecting and/or determining an agglomerate formed by the reaction between a specific binding agent and the corresponding acceptor substance according to the general methodology of blot overlay assays by using colloidal metal particles labelled components which may be visualized as a coloured signal at the surface of the blotting medium characteristric for the colloidal metal particles used or quantitatively determined following art-known spectrophotometric procedures such as densitometry.
Inventors
- 👤 Marc K. J. Moeremans
- 👤 Guido F. T. Daneels
- 👤 Jan R. De Mey
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 13:03
CPC Classifications
C; C12; C12Q; C12Q1/00; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/558; G01N33/559; Y; Y10; Y10S; Y10S436/00; Y10S436/805; Y10S436/807; Y10S436/824
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
76 words
A process for detecting or determining an acceptor substance in a blot overlay assay comprising the steps of: (i) immobilizing the acceptor substance to a blotting medium; (ii) contacting said blotting medium with colloidal metal particle labelled specific binding agent for said acceptor substance; and (iii) detecting the colored signal on the surface of the blotting medium caused by the colloidal metal particles at the reaction site between said acceptor substance and said specific binding agent.
Claim 2
Dependent Claim
76 words
A process for detecting or determining an acceptor substance in a blot overlay assay comprising the steps of: (i) immobilizing the acceptor substance to a blotting medium; (ii) contacting said blotting medium with colloidal metal particle labelled specific binding agent for said acceptor substance; and (iii) detecting the colored signal on the surface of the blotting medium caused by the colloidal metal particles at the reaction site between said acceptor substance and said specific binding agent.
Claim 3
Dependent Claim
48 words
A process according to claim 1 wherein the immobilization-step is effected by direct adsorption or covalent binding after applying a procedure of electrophoretic separation and applying a procedure of transfer or blotting from the electrophoretic medium to the blotting medium and subsequently quenching remaining agent specific binding sites.
Claim 4
Dependent Claim
46 words
A process according to claim 1 wherein the immobilization-step is effected by allowing the acceptor substance to become bound by a specific binding protein which has become immobilized to the blotting medium, by contacting said blotting medium with an aqueous solution which contains the acceptor substance.
Claim 5
Dependent Claim
39 words
A process according to any one of claims 1-3 wherein the colloidal metal particles consist of gold, silver, platinum, compounds of these metals, or iron or copper compounds having a particle size comprised between 3 nm and 100 nm.
Claim 6
Dependent Claim
30 words
A process according to any one of claims 1-3 wherein the colloidal metal particles consist of gold or silver having a particle size comprised between 3 nm and 100 nm.
Claim 7
Dependent Claim
42 words
A process according to claim 1 wherein the colloidal metal particles consist of gold or silver having particle size comprised between 3 nm and 100 nm and the final detection of the colloidal metal particles is effected after applying a physical developer.
Claim 8
Dependent Claim
14 words
A process according to claim 6 wherein the physical developer is a silver-containing compound.
Claim 9
Dependent Claim
86 words
A process for detecting or determining an acceptor substance in a blot overlay assay comprising the steps (i) immobilizing the acceptor substance to a blotting medium; (ii) contacting said blotting medium with an unlabeled specific binding agent for said acceptor substance and, subsequently, colloidal metal labelled protein specific for the unlabeled specific binding agent; and (iii) detecting the colored signal on the surface of the blotting medium caused by the colloidal metal particles at the reaction site between said acceptor substance and said specific binding agent.
Claim 10
Dependent Claim
86 words
A process for detecting or determining an acceptor substance in a blot overlay assay comprising the steps (i) immobilizing the acceptor substance to a blotting medium; (ii) contacting said blotting medium with an unlabeled specific binding agent for said acceptor substance and, subsequently, colloidal metal labelled protein specific for the unlabeled specific binding agent; and (iii) detecting the colored signal on the surface of the blotting medium caused by the colloidal metal particles at the reaction site between said acceptor substance and said specific binding agent.
Claim 11
Dependent Claim
48 words
A process according to claim 8 wherein the immobilization-step is effected by direct adsorption or covalent binding after applying a procedure of electrophoretic separation and applying a procedure of transfer or blotting from the electrophoretic medium to the blotting medium and subsequently quenching remaining agent specific binding sites.
Claim 12
Dependent Claim
46 words
A process according to claim 8 wherein the immobilization-step is effected by allowing the acceptor substance to become bound by a specific binding protein which has become immobilized to the blotting medium, by contacting said blotting medium with an aqueous solution which contains the acceptor substance.
Claim 13
Dependent Claim
40 words
A process according to any one of claims 8-10 wherein the colloidal metal particles consist of gold, silver, platinum or compounds of these metals, or iron, or copper compounds having a particle size comprised between 3 nm and 100 nm.
Claim 14
Dependent Claim
30 words
A process according to any one of claims 8-10 wherein the colloidal metal particles consist of gold or sivler having a particle size comprised between 3 nm and 100 nm.
Claim 15
Dependent Claim
43 words
A process according to claim 8 wherein the colloidal metal particles consist of gold or silver having a particle size comprised between 3 nm and 100 nm and the final detection of the colloidal metal particles is effected after applying a physical developer.
Claim 16
Dependent Claim
14 words
A processs according to claim 13 wherein the physical developer is a silver-compound compound.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-10-04 | 1992-04-04 | 1992-10-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-10-04 | 1996-04-04 | 1996-10-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-10-04 | 2000-04-04 | 2000-10-04 | $7700.00 | Window Closed / Lapsed |