US4775635A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Rapid assay processor
Assignee: Dade Behring Inc
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Filing: 1986-05-02
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Granted: 1988-10-04
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Expires: 2006-05-02
Abstract
Abstract A system and method is disclosed for the rapid detection and (or) purification of analytes in a sample. The system makes use of a fluid receptacle containing a capture reagent and a manifold having two ports. A sample containing an analyte disposed within the fluid receptacle is subjected to a capture reagent and processed by inserting the open end of the receptacle in one port. Vacuum, gas, reagents, and wash fluids are then applied to the second port to effect rapid interaction between the capture reagent and analyte, efficient washing of the capture reagent and detection and/or collection of the analyte. The system and method provide a rapid safe, uncomplicated means of automating processes based on affinity complexation principles. Encompassed within this category of processes are immunoassays, nucleic acid hybridization tests and affinity separations.
Inventors
- 👤 Richard C. Ebersole
- 👤 Jesse G. Forsythe, Jr.
- 👤 Frank T. Gelormini
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 10:15
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N35/00; G01N35/10; G01N2035/1027; G01N2035/1048; G01N2035/1055; Y; Y10; Y10S; Y10S436/00; Y10S436/807; Y10S436/815; Y10S436/817; Y10S436/818; Y10T; Y10T436/00; Y10T436/25; Y10T436/2575
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
109 words
A method for processing a sample having an analyte by the steps of: using a closed receptacle, the receptacle having a single access port positioned below the remainder of the receptacle, together with an immobilized capture reagent for the analyte, and a porous retainer able to pass easily fluids, cells and the like in the receptacle for securing the capture reagent, subjecting the access port and hence the receptacle to an initial vacuum of at least 25 inches of mercury, and thereafter selectively subjecting the access port to reagents, vacuum, air, and/or wash fluids while maintaining a vacuum within the receptacle thereby to capture, wash and develop the analyte.
Claim 2
Independent Claim
109 words
A method for processing a sample having an analyte by the steps of: using a closed receptacle, the receptacle having a single access port positioned below the remainder of the receptacle, together with an immobilized capture reagent for the analyte, and a porous retainer able to pass easily fluids, cells and the like in the receptacle for securing the capture reagent, subjecting the access port and hence the receptacle to an initial vacuum of at least 25 inches of mercury, and thereafter selectively subjecting the access port to reagents, vacuum, air, and/or wash fluids while maintaining a vacuum within the receptacle thereby to capture, wash and develop the analyte.
Claim 3
Dependent Claim
49 words
The method of claim 1 wherein the capture reagent is immobilized on the porous retainer and the receptacle access port is subjected to coordinated application of vacuum and air while retaining a vacuum within the receptacle to recycle the analyte and reagents back and forth across the capture reagent.
Claim 4
Dependent Claim
48 words
The method of claim 1 wherein the second step includes alternately introducing into and removing from the access port air to intermix the sample with the capture reagent while retaining a vacuum and the sample in the receptacle, thereby to capture the analyte on the immobilized capture reagent.
Claim 5
Dependent Claim
92 words
The method of claim 1 wherein the step of selectively subjecting the receptacle includes: (1) subjecting the access port to a vacuum sufficient to remove the sample, (2) while maintaining the access port below atmospheric pressure, washing the immobilized capture reagent by subjecting the access port to alternate bursts of air and wash fluid, (3) subjecting the access port to vacuum to remove the wash fluids, (4) introducing a signal generating reagent to the evacuated receptacle, and thereafter repeating step (2) and allowing the pressure in the receptacle to return to atmospheric.
Claim 6
Dependent Claim
23 words
The method of claim 1 wherein the capture reagent is immobilized on beads in the receptacle which cannot pass through the porous retainer.
Claim 7
Dependent Claim
125 words
The method of claim 1 the second step includes alternately introducing into and removing from the access port air to intermix the sample with the capture reagent while retaining a vacuum and the sample in the receptacle, thereby to capture the analyte on the immobilized capture reagent (1) subjecting the access port to a vacuum sufficient to remove the sample, (2) while maintaining the access port below atmospheric pressure, washing the immobilized capture reagent by subjecting the access port to alternate bursts of air and wash fluid, (3) subjecting the access port to vacuum to remove the wash fluids, (4) introducing a signal generating reagent to the evacuated receptacle, and thereafter repeating step (2) and allowing the pressure in the receptacle to return to atmospheric.
References Cited (Backward Citations)
US3748909A
Cited
US4071315A
Cited
US4117728A
Cited
US4248830A
Cited
US4225558A
Cited
US4454231A
Cited
JPS5822375A
Cited
US4477578A
Cited
DE3217032A1
Cited
US4483825A
Cited
US4424279A
Cited
US4458020A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-10-04 | 1992-04-04 | 1992-10-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-10-04 | 1996-04-04 | 1996-10-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-10-04 | 2000-04-04 | 2000-10-04 | $7700.00 | Window Closed / Lapsed |