US4775630A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Transcriptional control element adapted for regulation of gene expression in animal cells
Assignee: Vanderbilt University
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Filing: 1986-08-15
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Granted: 1988-10-04
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Expires: 2006-08-15
Abstract
Abstract A novel alteration of a viral DNA sequence, derived from a human adenovirus type 3 mutant (Ad3) designaed Ad3h15, provides a transcriptional control element which can be used to regulate expression of a selected gene in animal and human cells. The Ad3h15 control element blocks transcription of a controlled gene in the presence of the products of the Ad3 E1A gene, and amplifies transcription in the presence of type 5 (Ad5) adenovirus E1A gene products.
Inventors
- 👤 Clark Tibbetts
- 👤 Pamela L. Larsen
- 👤 Stephen N. Jones
- 👤 Mary M. McGrane
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 13:20
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/85
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
99 words
A hybrid DNA plasmid including a functional adenovirus transcriptional regulator region containing DNA sequences controlling expression of an adenovirus E1A gene, said controlling sequences including a transcriptional regulator sequence followed in tandem by a duplicate thereof, said regulator sequence responding to Ad3 E1A products to block the expression of a gene under its control and responding to Ad5 E1A gene products to unblock and amplify expression of the controlled gene, said tandem sequence consisting of double stranded DNA represented by CCATTTTCGC GCGAAAACTA AATGAGGAAG TGAATTTCTG AGTCATTTCG CGGTTATGCC, wherein A, T, G, and C respectively represent adenine, thymine, guanine and cystosine.
Claim 2
Dependent Claim
99 words
A hybrid DNA plasmid including a functional adenovirus transcriptional regulator region containing DNA sequences controlling expression of an adenovirus E1A gene, said controlling sequences including a transcriptional regulator sequence followed in tandem by a duplicate thereof, said regulator sequence responding to Ad3 E1A products to block the expression of a gene under its control and responding to Ad5 E1A gene products to unblock and amplify expression of the controlled gene, said tandem sequence consisting of double stranded DNA represented by CCATTTTCGC GCGAAAACTA AATGAGGAAG TGAATTTCTG AGTCATTTCG CGGTTATGCC, wherein A, T, G, and C respectively represent adenine, thymine, guanine and cystosine.
Claim 3
Dependent Claim
7 words
The plasmid pAd3h15 (ATCC Accession No. 53156).
Claim 4
Dependent Claim
7 words
The plasmid pAd3h15 (ATCC Accession No. 53156).
Claim 5
Dependent Claim
21 words
DNA fragments comprising the fragments obtained by cleaving plasmid pAd3h15 (ATCC Accession No. 53156) with the endonucleases EcoRI and HpA I.
Claim 6
Dependent Claim
21 words
DNA fragments comprising the fragments obtained by cleaving plasmid pAd3h15 (ATCC Accession No. 53156) with the endonucleases EcoRI and HpA I.
Claim 7
Dependent Claim
71 words
A double stranded DNA fragment including the sequence represented by: AACGG CTAAAAGGGG CGGCGCGACC GTGGGAAAAT GACGTGACTT ATGTGGGAGG AGTTATGTTG CAAGTTATTA CGGTAAATGT GACGTAAAAC GAGGTGTGGT TTGAACACGG AAGTAGACAG TTTTCCCACG CTTACTGACA GGATATGAGG TAGTTTTGGG CGGATGCAAG TGAAAATTCT CCATTTTCGC GCGAAAACTA AATGAGGAAG TGAATTTCTG AGTCATTTCG CGGTTATGCC CCATTTTCGC GCGAAAACTA AATGAGGAAG TGAATTTCTG AGTCATTTCG CGGTTATGCC AGGGTGGAGT ATTTGCCGAG GGCCGAGTAG ACTTTGACCG TTTACGTGGA GGTTTCGATT ACCGTGTTTT TCACCTAAAT TTCCGCGTAC GGTGTCAAAG TCCTGTGTTT TTACGTAGGT GTCAGCTGAT CGTCAGGGTA TTTAAACCTG ACGAGTTCCG CTAAGAGGCC wherein A, T, G, and C respectively represent adenine, thymine, guanine and cystosine.
Claim 8
Dependent Claim
71 words
A double stranded DNA fragment including the sequence represented by: AACGG CTAAAAGGGG CGGCGCGACC GTGGGAAAAT GACGTGACTT ATGTGGGAGG AGTTATGTTG CAAGTTATTA CGGTAAATGT GACGTAAAAC GAGGTGTGGT TTGAACACGG AAGTAGACAG TTTTCCCACG CTTACTGACA GGATATGAGG TAGTTTTGGG CGGATGCAAG TGAAAATTCT CCATTTTCGC GCGAAAACTA AATGAGGAAG TGAATTTCTG AGTCATTTCG CGGTTATGCC CCATTTTCGC GCGAAAACTA AATGAGGAAG TGAATTTCTG AGTCATTTCG CGGTTATGCC AGGGTGGAGT ATTTGCCGAG GGCCGAGTAG ACTTTGACCG TTTACGTGGA GGTTTCGATT ACCGTGTTTT TCACCTAAAT TTCCGCGTAC GGTGTCAAAG TCCTGTGTTT TTACGTAGGT GTCAGCTGAT CGTCAGGGTA TTTAAACCTG ACGAGTTCCG CTAAGAGGCC wherein A, T, G, and C respectively represent adenine, thymine, guanine and cystosine.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-10-04 | 1992-04-04 | 1992-10-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-10-04 | 1996-04-04 | 1996-10-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-10-04 | 2000-04-04 | 2000-10-04 | $7700.00 | Window Closed / Lapsed |