US4772547A
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US
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HTLV-III envelope peptides
Assignee: Roche Diagnostics Corp
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Filing: 1986-02-03
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Granted: 1988-09-20
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Expires: 2006-02-03
Abstract
Abstract The present disclosure relates to synthetic peptides derived from the conserved region of the HTLVIII envelope proteins. These peptides are useful as reagents for immunoassays for detection of AIDS antibodies, as components of immunogenic compositions useful as vaccines, and for the production of anti-bodies selective to said envelope protein and methods for detecting the presence of AIDS antibodies in biological fluid samples.
Inventors
- 👤 Edgar P. Heimer
- 👤 Premkumar E. Reddy
- 👤 Robert C. Gallo
- 👤 Flossie Wong-Staal
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 06:26
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/005; C12; C12N; C12N2740/00; C12N2740/00011; C12N2740/10011; C12N2740/16011; C12N2740/16111; C12N2740/16122; Y; Y10; Y10S; Y10S435/00; Y10S435/805; Y10S435/81; Y10S435/974; Y10S436/00; Y10S436/811; Y10S530/00; Y10S530/806; Y10S530/807; Y10S530/808; Y10S930/00; Y10S930/01; Y10S930/22; Y10S930/221
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
76 words
A peptide of the formula W-X-Lys-Ala-Lys-Arg-Arg-Val-Val-Gln-Arg-Glu-Lys-Arg-Y-Z where W is H-, Cys- or Tyr-, X is a bond or sub-sequence of one or more amino acids starting from carboxyl terminus of the sequence Asn-Asn-Asn-Asn-Gly-Ser-Glu-Ile-Phe-Arg-Pro-Gly-Gly-Gly-Asp-Met-Arg-Asp-Asn-Trp-Arg-Ser-Glu-Leu-Tyr-Lys-Tyr-Lys-Val-Val-Lys-Ile-Glu-Pro-Leu-Gly-Val-Ala-Pro-Thr, Y is a bond or a sub-sequence of one or more amino acids starting from the amino terminus of the sequence Ala-Val-Gly-Ile-Gly-Ala-Leu-Phe-Leu-Gly-Phe-Leu-Gly-Ala-Ala-Gly-Ser-Thr-Met-Gly-Ala-Ala-Ser-Met-Thr-Leu-Thr-Val-Gln-Ala-Arg-Gln-Leu-Leu-Ser-Gly-Ile-Val-Gln, and Z is -OH, NH 2 or -Cys-NH 2 , provided, however, that one of X or Y is a bond.
Claim 2
Dependent Claim
76 words
A peptide of the formula W-X-Lys-Ala-Lys-Arg-Arg-Val-Val-Gln-Arg-Glu-Lys-Arg-Y-Z where W is H-, Cys- or Tyr-, X is a bond or sub-sequence of one or more amino acids starting from carboxyl terminus of the sequence Asn-Asn-Asn-Asn-Gly-Ser-Glu-Ile-Phe-Arg-Pro-Gly-Gly-Gly-Asp-Met-Arg-Asp-Asn-Trp-Arg-Ser-Glu-Leu-Tyr-Lys-Tyr-Lys-Val-Val-Lys-Ile-Glu-Pro-Leu-Gly-Val-Ala-Pro-Thr, Y is a bond or a sub-sequence of one or more amino acids starting from the amino terminus of the sequence Ala-Val-Gly-Ile-Gly-Ala-Leu-Phe-Leu-Gly-Phe-Leu-Gly-Ala-Ala-Gly-Ser-Thr-Met-Gly-Ala-Ala-Ser-Met-Thr-Leu-Thr-Val-Gln-Ala-Arg-Gln-Leu-Leu-Ser-Gly-Ile-Val-Gln, and Z is -OH, NH 2 or -Cys-NH 2 , provided, however, that one of X or Y is a bond.
Claim 3
Dependent Claim
12 words
The peptide of claim 1 where both X and Y are bonds.
Claim 4
Dependent Claim
15 words
The peptide of claim 2, wherein W is H- and Z is -Cys-NH 2 .
Claim 5
Dependent Claim
15 words
The peptide of claim 2 wherein W is Cys and Z is -NH 2 .
Claim 6
Dependent Claim
9 words
The peptide of claim 2, wherein W is Tyr-.
Claim 7
Dependent Claim
9 words
A peptide of claim 5 labelled with 125 I.
Claim 8
Dependent Claim
15 words
The peptide of claim 1 where X is said sub-sequence and Y is a bond.
Claim 9
Dependent Claim
9 words
The peptide of claim 7 where X is Lys-Val-Val-Lys-Ile-Glu-Pro-Leu-Gly-Val-Ala-Pro-Thr.
Claim 10
Dependent Claim
10 words
The peptide of claim 8 which is Lys-Val-Val-Lys-Ile-Glu-Pro-Leu-Gly-Val-Ala-Pro-Thr-Lys-Ala-Lys-Arg-Arg-Val-Val-Gln-Arg-Glu-Lys-Arg-Cys-NH 2 .
Claim 11
Dependent Claim
10 words
The peptide of claim 8 which is Tyr-Lys-Val-Val-Lys-Ile-Glu-Pro-Leu-Gly-Val-Ala-Pro-Thr-Lys-Ala-Lys-Arg-Arg-Val-Val-Gln-Arg-Glu-Lys-Arg-Cys-NH 2 .
Claim 12
Dependent Claim
24 words
An immunogen composition comprising a peptide of claim 1, where one of W or Z is Cys covalently bonded to an immunogenic carrier material.
Claim 13
Dependent Claim
18 words
The composition of claim 11, wherein said peptide has W as H- and Z as -Cys-NH 2 .
Claim 14
Dependent Claim
14 words
The composition of claim 11, wherein said immunogenic carrier material is keyhole limpet hemocyanin.
Claim 15
Dependent Claim
57 words
An antibody which selectively binds to a peptide of the formula W-X-Lys-Ala-Lys-Arg-Arg-Val-Val-Gln-Arg-Glu-Lys-Arg-Z, where W is H-, Cys- or Tyr-, X is a bond or a sub-sequence of one or more amino acids starting from carboxyl terminus of the sequence Asn-Asn-Asn-Asn-Gly-Ser-Glu-ILe-Phe-Arg-Pro-Gly-Gly-Gly-Asp-Met-Arg-Asp-Asn-Trp-Arg-Ser-Glu-Leu-Tyr-Lys-Tyr-Lys-Val-Val-Lys-Ile-Glu-Pro-Leu-Gly-Val-Ala-Pro-Thr, and Z is -OH, -NH 2 or -Cys-NH 2 ; HTLV-III env protein or an AIDS virus.
Claim 16
Dependent Claim
57 words
An antibody which selectively binds to a peptide of the formula W-X-Lys-Ala-Lys-Arg-Arg-Val-Val-Gln-Arg-Glu-Lys-Arg-Z, where W is H-, Cys- or Tyr-, X is a bond or a sub-sequence of one or more amino acids starting from carboxyl terminus of the sequence Asn-Asn-Asn-Asn-Gly-Ser-Glu-ILe-Phe-Arg-Pro-Gly-Gly-Gly-Asp-Met-Arg-Asp-Asn-Trp-Arg-Ser-Glu-Leu-Tyr-Lys-Tyr-Lys-Val-Val-Lys-Ile-Glu-Pro-Leu-Gly-Val-Ala-Pro-Thr, and Z is -OH, -NH 2 or -Cys-NH 2 ; HTLV-III env protein or an AIDS virus.
Claim 17
Dependent Claim
10 words
The antibody of claim 14, which is a polyclonal antibody.
Claim 18
Dependent Claim
10 words
The antibody of claim 14 which is a monoclonal antibody.
Claim 19
Dependent Claim
120 words
A method of testing human blood for the presence of antibodies to the viral etiological agent of AIDS which comprises: (a) contacting a peptide of claim 1 which is bound to a solid support with a test sample of human blood, whereby any antibodies to the viral etiological agent of AIDS present in the test sample will bind to the peptide; (b) removing the test sample; (c) washing the solid support to remove unbound antibodies; (d) adding a reagent capable of selectively detecting human antibodies to the washed support step (c), whereby the reagent will bind to any antibodies bound to the support; (e) washing the support of step (d) to remove unbound reagent; and (f) detecting the bound reagent.
Claim 20
Dependent Claim
48 words
The method of claim 17, wherein said solid support is a plastic microtiter plate and said reagent capable of detecting human antibodies is anti-human IgG labelled with horseradish peroxidase which is reacted with a substrate which provides a color change end point in the presence of said peroxidase.
Claim 21
Dependent Claim
12 words
The method of claim 17, wherein said peptide is Lys-Val-Val-Lys-Ile-Glu-Pro-Leu-Gly-Val-Ala-Pro-Thr-Lys-Ala-Lys-Arg-Arg-Val-Val-Gln-Arg-Glu-Lys-Arg-Cys-NH 2 .
Claim 22
Dependent Claim
12 words
The method of claim 17, wherein said peptide is Cys-Lys-Ala-Lys-Arg-Arg-Val-Val-Gln-Arg-Glu-Lys-Arg-NH 2 .
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-09-20 | 1992-03-21 | 1992-09-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-09-20 | 1996-03-21 | 1996-09-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-09-20 | 2000-03-21 | 2000-09-20 | $7700.00 | Window Closed / Lapsed |