US4772397A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for removing nucleic acids and purine bases from gelatin
Assignee: Forte Fotokemial Idpar
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Filing: 1987-06-12
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Granted: 1988-09-20
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Expires: 2007-06-12
Abstract
Abstract The invention relates to a process for removing nucleic acids and purine bases from gelatine by (a) adding cyclodextrin bead polymer having a particle size of 20 to 600 μ, a cyclodextrin content of 30 to 70% and a polyvinyl alcohol content of 0.3 to 0.5% to the gelatin solution in a weight ratio of 1:1 to 10:1 calculated on the amount of the gelatin then filtering off the cyclodextrin polymer or (b) passing the gelatin solution through a cyclodextrin bead polymer column, whereby the amount of the gelatin is 5 to 50% of the weight of the column charge, then eluting the nucleic acids and the purine bases from the cyclodextrin bead polymer.
Inventors
- 👤 Miklos Szucs
- 👤 Istvanne Palotas
- 👤 Jozsef Szejtli
- 👤 Eva Fenyves
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 15:15
CPC Classifications
C; C09; C09H; C09H3/00; C09H3/02; G; G03; G03C; G03C1/00; G03C1/005; G03C1/04; G03C1/047
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
77 words
A process for removing nucleic acids and purine bases from gelatin comprising bringing into contact a gelatin solution and a cross-linked beta-cyclodextrin bead polymer having a particle size of about 20 to 600 microns, a beta-cyclodextrin content of about 30% to 70% by weight and a polyvinyl alcohol content of about 0.3% to 0.5% by weight; separating said cross-linked polymer and said gelatin solution to obtain a gelatin solution substantially free from nucleic acids and purine bases.
Claim 2
Dependent Claim
77 words
A process for removing nucleic acids and purine bases from gelatin comprising bringing into contact a gelatin solution and a cross-linked beta-cyclodextrin bead polymer having a particle size of about 20 to 600 microns, a beta-cyclodextrin content of about 30% to 70% by weight and a polyvinyl alcohol content of about 0.3% to 0.5% by weight; separating said cross-linked polymer and said gelatin solution to obtain a gelatin solution substantially free from nucleic acids and purine bases.
Claim 3
Dependent Claim
45 words
A process of claim 1 wherein said contacting is accomplished by adding said polymer to a gelatin solution in a weight ratio of polymer to gelatin of about 1:1 to 10:1 and said separation is accomplished by filtering off the polymer from the gelatin solution.
Claim 4
Dependent Claim
30 words
A process of claim 2 wherein said polymer has a particle size of about 90 microns to 300 microns and a beta-cyclodextrin content of about 50% to 60% by weight.
Claim 5
Dependent Claim
56 words
A process of claim 1 wherein said contacting is accomplished by passing a gelatin solution through a column of said crosslinked beta-cyclodextrin bead polymer in an amount of about 5% to 50% of the dry weight of the polymer in the column; then collecting the eluted gelatin solution substantially free of nucleic acids and purine bases.
Claim 6
Dependent Claim
26 words
A process of claim 4 wherein the amount of gelatin used is about 10% to 30% of the dry weight of the polymer in the column.
References Cited (Backward Citations)
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-09-20 | 1992-03-21 | 1992-09-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-09-20 | 1996-03-21 | 1996-09-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-09-20 | 2000-03-21 | 2000-09-20 | $7700.00 | Window Closed / Lapsed |