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US4771001A Expired / Lapsed US ⏱️ 0.0 years remaining

Production of lactic acid by continuous fermentation using an inexpensive raw material and a simplified method of lactic acid purification

Assignee: Golden Technologies Co Inc
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Filing: 1986-09-24
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Granted: 1988-09-13
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Expires: 2006-09-24
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Abstract

Abstract The disclosure describes a method for producing lactic acid from carbohydrate-containing media by continuous fermentation utilizing particular methods of media pretreatment, cell-recycle fermentation, fermentation broth acidification, and lactic acid separation.

Inventors

  • 👤 Richard B. Bailey
  • 👤 Dilip K. Joshi
  • 👤 Stephen L. Michaels
  • 👤 Richard A. Wisdom

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-10-29 18:34
CPC Classifications
C; C12; C12P; C12P7/00; C12P7/40; C12P7/56; A; A23; A23C; A23C21/00; A23C21/02; A23C21/023; Y; Y10; Y10S; Y10S435/00; Y10S435/813; Y10S435/8215; Y10S435/822; Y10S435/853; Y10S435/854; Y10S435/856; Y10S435/885

Claims Analysis

Total Claims: 23
Claim 1
Independent Claim 207 words
A process for producing lactic acid from a carbohydrate-containing medium comprising the steps of: (a) fermenting said carbohydrate-containing medium with a lactic acid or lactic acid salt-producing microorganism in a fermentation ot form a broth; (b) continuously removing a product-containing both from said zone, removing cells of said microorganism from said broth to form a cell-free product-containing stream and recycling said cells to said zone; (c) contacting said cell-free product-containing stream at an acidic pH with a water-immiscible liquid consisting essentially of trialkyl tertiary amines of at least 24 carbon atoms and a water-immiscible organic solvent for a period of time and at a temperature sufficient to extract lactic acid and lactic acid salt from said stream into a water-immiscible phase, said liquid characterized by a distribution coefficient in excess of 1.0 for lactic acid and lactic acid salt in said liquid versus water; (d) separating said water-immiscible phase from the mixture formed in step (c); (e) contacting said water-immiscible liquid from step (d) with a material selected from the group consisting of aqueous ammonium hydroxide, solid alkalis, solid alkaline earth compounds, and aqueous non-homogenous suspensions of solid alkalis or solid alkaline earth compounds, whereby lactic acid and lactic acid salt are removed from said water-immiscible phase.
Claim 2
Dependent Claim 207 words
A process for producing lactic acid from a carbohydrate-containing medium comprising the steps of: (a) fermenting said carbohydrate-containing medium with a lactic acid or lactic acid salt-producing microorganism in a fermentation ot form a broth; (b) continuously removing a product-containing both from said zone, removing cells of said microorganism from said broth to form a cell-free product-containing stream and recycling said cells to said zone; (c) contacting said cell-free product-containing stream at an acidic pH with a water-immiscible liquid consisting essentially of trialkyl tertiary amines of at least 24 carbon atoms and a water-immiscible organic solvent for a period of time and at a temperature sufficient to extract lactic acid and lactic acid salt from said stream into a water-immiscible phase, said liquid characterized by a distribution coefficient in excess of 1.0 for lactic acid and lactic acid salt in said liquid versus water; (d) separating said water-immiscible phase from the mixture formed in step (c); (e) contacting said water-immiscible liquid from step (d) with a material selected from the group consisting of aqueous ammonium hydroxide, solid alkalis, solid alkaline earth compounds, and aqueous non-homogenous suspensions of solid alkalis or solid alkaline earth compounds, whereby lactic acid and lactic acid salt are removed from said water-immiscible phase.
Claim 3
Dependent Claim 196 words
A process for producing lactic acid from a carbohydrate-containing medium comprising the steps of: (a) fermenting said carbohydrate-containing medium with a lactic acid or lactic acid salt-producing microorganism in a fermentation zone; (b) removing a product-containing both from said zone, removing cells of said microorganism from said broth to form a cell-free product-containing stream; (c) contacting said cell-free product-containing stream at an acidic pH with a water-immiscible liquid consisting essentially of trialkyl tertiary amines of at least 24 carbon atoms and a water-immiscible organic solvent for a period of time and at a temperature sufficient to extract lactic acid and lactic acid salt from said stream into a water-immiscible phase, said liquid characterized by a distribution coefficient in excess of 1.0 for lactic acid and lactic acid salt in said liquid versus water; (d) separating said water-immiscible phase from the mixture formed in step (c); (e) contacting said water-immiscible liquid from step (d) with a material selected from the group consisting of aqueous ammonium hydroxide, solid alkalis, solid alkaline earth compounds, and aqueous non-homogenous suspensions of solid alkalis or solid alkaline earth compounds, whereby lactic acid and lactic acid salt are removed from said water-immiscible phase.
Claim 4
Dependent Claim 196 words
A process for producing lactic acid from a carbohydrate-containing medium comprising the steps of: (a) fermenting said carbohydrate-containing medium with a lactic acid or lactic acid salt-producing microorganism in a fermentation zone; (b) removing a product-containing both from said zone, removing cells of said microorganism from said broth to form a cell-free product-containing stream; (c) contacting said cell-free product-containing stream at an acidic pH with a water-immiscible liquid consisting essentially of trialkyl tertiary amines of at least 24 carbon atoms and a water-immiscible organic solvent for a period of time and at a temperature sufficient to extract lactic acid and lactic acid salt from said stream into a water-immiscible phase, said liquid characterized by a distribution coefficient in excess of 1.0 for lactic acid and lactic acid salt in said liquid versus water; (d) separating said water-immiscible phase from the mixture formed in step (c); (e) contacting said water-immiscible liquid from step (d) with a material selected from the group consisting of aqueous ammonium hydroxide, solid alkalis, solid alkaline earth compounds, and aqueous non-homogenous suspensions of solid alkalis or solid alkaline earth compounds, whereby lactic acid and lactic acid salt are removed from said water-immiscible phase.
Claim 5
Dependent Claim 44 words
A process according to claim 1 or 2 wherein in step (a) said lactic acid or lactic acid salt-producing microorganism is a microorganism that produces primarily D(-)lactic acid or lactate, L(+)lactic acid or lactate, or a D(-)L(+) racemic mixture of lactic acid or lactate.
Claim 6
Dependent Claim 30 words
A process according to claim 1 or 2 wherein in step (a) said lactic acid or lactic acid salt-producing microorganism is a species selected from the genus Lactobacillus or Streptococcus.
Claim 7
Dependent Claim 25 words
A process according to claim 1 or 2 wherein in step (b) said removal of said cells from said broth is accomplished by cross-flow microfiltration.
Claim 8
Dependent Claim 35 words
A process according to claim 1 or 2 wherein in step (b) said removal of said cells from said broth is accomplished by ultrafiltration with a filter whose molecular weight cutoff is greater than 100,000.
Claim 9
Dependent Claim 24 words
A process according to claim 1 or 2 wherein in step (b) said removal of said cells from said broth is accomplished by centrifugation.
Claim 10
Dependent Claim 48 words
A process according to claim 1 or 2 further comprising the step of increasing the ionic salt concentration of said aqueous solution prior to step (c) by adding a salt selected from the group consisting of chlorides, phosphates and sulfates of ammonium, alkali, metals and alkaline earth metals.
Claim 11
Dependent Claim 26 words
A process according to claim 1 or 2 wherein in step (c) said product-containing stream is at a pH in the range of 0.5 to 6.0.
Claim 12
Dependent Claim 31 words
A process according to claim 1 or 2 wherein in step (c) said water-immiscible organic solvent is selected from the group consisting of C8 to C14 aliphatic ketones and halogenated hydrocarbons.
Claim 13
Dependent Claim 18 words
A process according to claim 1 or 2 wherein in step (e) said material comprises aqueous ammonium hydroxide.
Claim 14
Dependent Claim 25 words
A process according to claim 1 or 2 wherein said step (e) comprises contacting said water immiscible phase with solid alkali or alkaline earth compound.
Claim 15
Dependent Claim 31 words
A process according to claim 12 wherein said alkali or alkaline earth compound is selected from the group consisting of hydroxides, oxides and carbonates of calcium, magnesium, iron, zinc, and aluminum.
Claim 16
Dependent Claim 40 words
A process according to claim 1 or 2 further comprising the step of, prior to said step (c), contacting said stream with an ion exchange resin to adjust said stream to a pH in the range of 0.5 to 6.0.
Claim 17
Dependent Claim 25 words
A process according to claim 1 or 2 wherein in step (c) said tertiary amine comprises a trialkyl amine and said solvent comprises isobutyl-heptyl ketone.
Claim 18
Dependent Claim 24 words
A process according to claim 1 or 2 wherein in step (c) said tertiary amine comprises a trialkyl amine and said solvent comprises 2-octanone.
Claim 19
Dependent Claim 19 words
A process according to claim 15 wherein said step (e) comprises contacting said water-immiscible phase with aqueous ammonium hydroxide.
Claim 20
Dependent Claim 26 words
A process according to claim 15 wherein said step (e) comprises contacting said water-immiscible phase with said aqueous suspension of said alkali or alkaline earth compound.
Claim 21
Dependent Claim 32 words
A process according to claim 1 or 2 wherein in step (c) said tertiary amine comprises a trialkyl amine and said solvent comprises a mixture of a ketone and a phosphoryl oxide.
Claim 22
Dependent Claim 35 words
A process according to claim 1 or 2 wherein in said step (e) said contact occurs at the same or at a lower temperature than the temperature at which the extraction step (c) is performed.
Claim 23
Dependent Claim 14 words
A process according to claim 1 or 2 wherein said lactic acid is heat-stable.

USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 1991-09-13 1992-03-14 1992-09-13 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 1995-09-13 1996-03-14 1996-09-13 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 1999-09-13 2000-03-14 2000-09-13 $7700.00 Window Closed / Lapsed

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