US4770995A
Expired / Lapsed
US
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Detection of the sensitivity of cells to the effects of tumor necrosis factor and lymphotoxin
Assignee: New York Blood Center
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Filing: 1985-08-29
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Granted: 1988-09-13
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Expires: 2005-08-29
Abstract
Abstract A process for predicting the sensitivity of cells, e.g., tumor cells, to the effects of tumor necrosis factor or lymphotoxin involving ascertaining the binding of the tumor necrosis factor or lymphotoxin to the cells, i.e., measuring the number of receptors on the cells.
Inventors
- 👤 Berish Y. Rubin
- 👤 Sylvia L. Anderson
- 👤 Susan A. Sullivan
- 👤 Lloyd J. Old
- 👤 Barbara D. Williamson
- 👤 Elizabeth C. Richards
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 22:53
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/18; C07K16/28; C07K16/2866; C07K14/00; C07K14/435; C07K14/52; C07K14/525; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/5005; G01N33/5008; G01N33/5011
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
50 words
A process for predicting the sensitivity of cells to the effects of tumor necrosis factor or lymphotoxin comprising measuring the number of tumor necrosis factor or lymphotoxin receptors on said cells, the number of said receptors being correlected to the sensitivity of the cells to tumor necrosis factor or lymphotoxin.
Claim 2
Dependent Claim
50 words
A process for predicting the sensitivity of cells to the effects of tumor necrosis factor or lymphotoxin comprising measuring the number of tumor necrosis factor or lymphotoxin receptors on said cells, the number of said receptors being correlected to the sensitivity of the cells to tumor necrosis factor or lymphotoxin.
Claim 3
Dependent Claim
12 words
A process according to claim 1, wherein said cells are tumor cells.
Claim 4
Dependent Claim
51 words
A process according to claim 1, wherein said measuring of the number of receptors on said cells is accomplished by: a. incubating said cells with tumor necrosis factor or lymphotoxin labeled with a marker substance, and b. ascertaining if the labeled tumor necrosis factor or lymphotoxin specifically binds to the cells.
Claim 5
Dependent Claim
17 words
A process according to claim 2, wherein said tumor cells are in the form of tumor tissue.
Claim 6
Dependent Claim
28 words
A process according to claim 3, wherein said marker substance is selected from the group consisting of a radioactive moiety, an enzyme, a fluorescent moiety and a dyestuff.
Claim 7
Dependent Claim
14 words
A process according to claim 3, wherein said marker substance is I 125 .
Claim 8
Dependent Claim
55 words
A process according to claim 1, wherein said measuring of the number of receptors on said cells is accomplished by incubating a specific antibody to tumor necrosis factor receptor or lymphotoxin receptor with said cells, said antibody labeled with a marker substance, harvesting said cells and determining the amount of marker on the harvested cells.
Claim 9
Dependent Claim
27 words
A process according to claim 7, wherein said marker is selected from the group consisting of a radioactive moiety, an enzyme, a fluorescent moiety and a dyestuff.
Claim 10
Dependent Claim
13 words
A process according to claim 7, wherein said marker is I 125 .
Claim 11
Dependent Claim
145 words
A process for predicting the sensitivity of tumor cells to the effects of tumor necrosis factor or lymphotoxin comprising: a. incubating a first portion of said cells with tumor necrosis factor or lymphotoxin, labeled with a marker substance, b. incubating a second portion of said cells with unlabeled tumor necrosis factor or lymphotoxin and with labeled tumor necrosis factor or lymphotoxin, and c. harvesting the cells from step a, d. determining the amount of marker substance on the harvested cells from step c, e. harvesting the cells from step b, f. determining the amount of marker substance on the harvested cells from step e, and g. comparing the amounts of marker substance on the harvested cells from steps d and f, such that if the amounts from steps d and f are approximately the same, then the cells are not sensitive to TNF or LT.
Claim 12
Dependent Claim
145 words
A process for predicting the sensitivity of tumor cells to the effects of tumor necrosis factor or lymphotoxin comprising: a. incubating a first portion of said cells with tumor necrosis factor or lymphotoxin, labeled with a marker substance, b. incubating a second portion of said cells with unlabeled tumor necrosis factor or lymphotoxin and with labeled tumor necrosis factor or lymphotoxin, and c. harvesting the cells from step a, d. determining the amount of marker substance on the harvested cells from step c, e. harvesting the cells from step b, f. determining the amount of marker substance on the harvested cells from step e, and g. comparing the amounts of marker substance on the harvested cells from steps d and f, such that if the amounts from steps d and f are approximately the same, then the cells are not sensitive to TNF or LT.
Claim 13
Dependent Claim
28 words
A process according to claim 10, wherein the marker substance is selected from the group consisting of a radioactive moiety, an enyzme, a fluorescent moiety and a dyestuff.
Claim 14
Dependent Claim
14 words
A process according to claim 10, wherein said marker substance is I 125 .
Claim 15
Dependent Claim
17 words
A process according to claim 10, wherein said tumor cells are in the form of tumor tissue.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-09-13 | 1992-03-14 | 1992-09-13 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-09-13 | 1996-03-14 | 1996-09-13 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-09-13 | 2000-03-14 | 2000-09-13 | $7700.00 | Window Closed / Lapsed |